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US5741700A
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Method of immobilizing water-soluble bioorganic compounds on a capillary-porous carrier
Assignee: INSTITUT MOLEKULYARNOI BIOLOGII IM VA ENGELTARDA ROSSIISKOI AKADEMII NAUK
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Filing: 1994-08-05
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Granted: 1998-04-21
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Expires: 2014-08-05
Abstract
Abstract The method for immobilizing water-soluble bioorganic compounds to capillary-porous carrier comprises application of solutions of water-soluble bioorganic compounds onto a capillary-porous carrier, setting the carrier temperature equal to or below the dew point of the ambient air, keeping the carrier till appearance of water condensate and complete swelling of the carrier, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and is allowed to stand till completion of the chemical reaction of bonding the bioorganic compounds with the carrier.
Inventors
- 👤 Gennady Moiseevich Ershov
- 👤 Eduard Nikolaevich Timofeev
- 👤 Igor Borisovich Ivanov
- 👤 Vladimir Leonidovich Florentiev
- 👤 Andrei Darievich Mirzabekov
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; B; B01; B01J; B01J19/00; B01J19/0046; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00497; B01J2219/00527; B01J2219/00583; B01J2219/00585; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00646; B01J2219/00648; B01J2219/0065; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
116 words
A method for immobilizing water-soluble bioorganic compounds on to a carrier bondable with the compounds, comprising applying water-soluble bioorganic compounds onto a bondable carrier having a matrix of cells and kept thereon until completion of the chemical bonding between said bioorganic compounds and said carrier matrix, application of water-soluble bioorganic solutions being followed by setting the temperature of the carrier equal to or below the dew point of the ambient air and the carrier is allowed to stand until water condensate swells said matrix cells, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and allowed to stand until the reaction of bonding the bioorganic compounds with the carrier is completed.
Claim 2
Independent Claim
116 words
A method for immobilizing water-soluble bioorganic compounds on to a carrier bondable with the compounds, comprising applying water-soluble bioorganic compounds onto a bondable carrier having a matrix of cells and kept thereon until completion of the chemical bonding between said bioorganic compounds and said carrier matrix, application of water-soluble bioorganic solutions being followed by setting the temperature of the carrier equal to or below the dew point of the ambient air and the carrier is allowed to stand until water condensate swells said matrix cells, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and allowed to stand until the reaction of bonding the bioorganic compounds with the carrier is completed.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the bioorganic compounds are selected from the group consisting of oligonucleotides and polynucleotides.
Claim 4
Dependent Claim
12 words
A method according to claim 1 wherein said carrier comprises polyacrylamide gel.
Claim 5
Dependent Claim
19 words
A method according to claim 1 wherein said carrier is kept under said oil layer for about 48 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-04-20 | 2001-10-20 | 2002-04-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-04-20 | 2005-10-20 | 2006-04-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-04-20 | 2009-10-20 | 2010-04-21 | $7700.00 | Window Closed / Lapsed |