US5733563A
Expired / Lapsed
US
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Albumin based hydrogel
Assignee: RBA Pharma Inc
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Filing: 1996-01-23
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Granted: 1998-03-31
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Expires: 2016-01-23
Abstract
Abstract Novel bioartificial hydrogels consisting of a three-dimensional crosslinked mixture of: (a) a bifunctionalized polyethylene oxide, activated with an activating agent, dissolved in an aqueous solution; and (b) albumin type protein. The novel hydrogels are based on the crosslinking of albumin type protein of various sources including, for example, bovine serum albumin, lactalbumin or ovalbumin, with a bifunctionalyzed polyethylene oxide, most preferably polyethylene glycol, or a mixture of bifunctionalyzed polyethylene oxides preferably polyethylene glycol, of various molecular masses (Mr 2,000 to 35,000), dissolved in aqueous solution in adequate proportions. Also provided is a method and conditions for preparing the novel hydrogels. Also divulged are a variety of biomedical applications for the novel hydrogels. Additionally, it has been found that the mechanical properties of the novel hydrogels can be improved by adding to the casting solution unreactive polyethylen glycol or other inert polymer of high molecular masses (Mr>100,000). In general terms the novel hydrogels possess advantageous properties such as shape retention and shape memory, high water content (more than 94% (w/w) based on the dry weight of the hydrogel), good mechanical and optical properties.
Inventors
- 👤 Guy Fortier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:55
CPC Classifications
G; G02; G02B; G02B1/00; G02B1/04; G02B1/041; G02B1/043; A; A61; A61L; A61L15/00; A61L15/16; A61L15/22; A61L15/26; A61L15/32; A61L15/42; A61L15/60; C; C08; C08H; C08H1/00; C08J; C08J3/00; C08J3/02; C08J3/03; C08J3/075; C08L; C08L89/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
95 words
A polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 2
Dependent Claim
95 words
A polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 3
Dependent Claim
16 words
The hydrogel of claim 1, wherein said polyethylene oxide is an activated form of polyethylene glycol.
Claim 4
Dependent Claim
22 words
The hydrogel of claim 1, wherein said protein albumin is selected from the group consisting of bovine serum albumin, lactalbumin and ovalbumin.
Claim 5
Dependent Claim
22 words
The hydrogel of claim 2, wherein said protein albumin is selected from the group consisting of bovine serum albumin, lactalbumin and ovalbumin.
Claim 6
Dependent Claim
110 words
A polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800, and further wherein said mixture includes nonreactive polymers having a molecular mass greater than 100,000.
Claim 7
Dependent Claim
110 words
A polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800, and further wherein said mixture includes nonreactive polymers having a molecular mass greater than 100,000.
Claim 8
Dependent Claim
12 words
The hydrogel of claim 5, wherein said unreactive polymers are polyethylene glycol.
Claim 9
Dependent Claim
105 words
A contact lens device, wherein said contact lens device is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 10
Dependent Claim
105 words
A contact lens device, wherein said contact lens device is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 11
Dependent Claim
110 words
An ophthalmic device designed for use in an ophthalmic environment, wherein said ophthalmic device is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 12
Dependent Claim
110 words
An ophthalmic device designed for use in an ophthalmic environment, wherein said ophthalmic device is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 13
Dependent Claim
51 words
A method of administering a physiologically active compound to a mammal comprising the steps of: (a) loading the hydrogel of claim 1 with a solution comprising a therapeutically effective amount of said physiologically active compound, so that a loaded hydrogel is obtained; and (b) administering said loaded hydrogel to said mammal.
Claim 14
Dependent Claim
11 words
The method of claim 9, further comprising a physiologically acceptable carrier.
Claim 15
Dependent Claim
31 words
The method of claim 9, wherein said loaded hydrogel is a drug releasing composition having a physiologically acceptable form that is suitable for oral, rectal, cutaneous patch or sub-cutaneous implant administration.
Claim 16
Dependent Claim
13 words
The method of claim 9, wherein said loaded hydrogel is a wound dressing.
Claim 17
Dependent Claim
12 words
The method of claim 9, wherein said loaded hydrogel is artificial skin.
Claim 18
Dependent Claim
106 words
A matrix for immobilizing cells or enzymes, wherein said matrix is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 19
Dependent Claim
106 words
A matrix for immobilizing cells or enzymes, wherein said matrix is a polymerized, hydrophilic, water-swellable, and water insoluble hydrogel consisting essentially of a cross-linked mixture of an aqueous solution of activated bifunctionalized polyethylene oxide and protein albumin, wherein said activated bifunctional polyethylene oxide consists of a polyethylene derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group in an aqueous solution, said functionalized group not requiring a catalyst or activator to react with said amino, S--H, OH or COOH group, and n is from 45 to 800.
Claim 20
Dependent Claim
93 words
A process for preparing an albumin-based hydrogel comprising the steps of: (a) mixing a protein albumin solution with an activated polyethylene glycol derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group, and n is from 45 to 800; and (b) agitating the mixture of step (a) at a pH of about 7.8 to 10.3 until a hydrogel results from the cross-linking of said protein albumin with said polyethylene glycol derivative of formula (I).
Claim 21
Dependent Claim
93 words
A process for preparing an albumin-based hydrogel comprising the steps of: (a) mixing a protein albumin solution with an activated polyethylene glycol derivative of formula (I): X--O--(CH.sub.2 CH.sub.2 O).sub.n --X (I), wherein X represents a functionalized group capable of reacting with an amino, an S--H, an OH or a COOH group, and n is from 45 to 800; and (b) agitating the mixture of step (a) at a pH of about 7.8 to 10.3 until a hydrogel results from the cross-linking of said protein albumin with said polyethylene glycol derivative of formula (I).
Claim 22
Dependent Claim
18 words
The process of claim 15, wherein step (a) includes nonreactive polymers having a molecular mass greater than 100,000.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-03-30 | 2001-09-29 | 2002-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-30 | 2005-09-29 | 2006-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-30 | 2009-09-29 | 2010-03-31 | $7700.00 | Window Closed / Lapsed |