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US5723588A
Expired / Lapsed
US
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Protein-enriched thermoplastics
Assignee: Protein Polymer Technologies Inc
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Filing: 1993-07-09
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Granted: 1998-03-03
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Expires: 2013-07-09
Abstract
Abstract Thermoplastics interdispersed with a variety of functional thermostable polypeptides, including proteins, and methods of making such thermoplastics are provided. The disclosure demonstrates that certain polypeptides can retain functional activity through exposure to plastic thermomolding. The polypeptides are exposed to the heating and molding/extrusion/casting process and are hence present on the formed plastic surface and at a depth below the plastic surface. The polypeptides contained in the disclosed compositions retain functional properties or binding specificities through the heating and molding/extrusion/casting processes. Preferred thermostable polypeptides used in the disclosed compositions include silk-like protein polymers, particularly ProNectin TM F. The disclosed methods and compositions find use in many applications where plastics containing functional thermostable polypeptides are desired, in particular, cell cultureware.
Inventors
- 👤 David A. Donofrio
- 👤 Erwin R. Stedronsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:02
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L17/00; A61L29/00; A61L29/04; A61L29/049; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43563; C07K14/43586; C07K14/78; C07K17/00; C07K17/02; C07K17/08; C12N1/00; C12N1/06; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/66; C12N15/70; C12N15/74; C12N15/75; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/33; C07K2319/61; C12N2533/00; C12N2533/30; C12N2533/50; C12N2533/52; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
69 words
A composition comprising a biologically functional thermostable polypeptide interdispersed with a thermoplastic resulting from a melt at a temperature of at least 60° C., wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 2
Dependent Claim
69 words
A composition comprising a biologically functional thermostable polypeptide interdispersed with a thermoplastic resulting from a melt at a temperature of at least 60° C., wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 3
Dependent Claim
19 words
The composition of claim 1, wherein said intervening sequence is from 3 to about 60 amino acids in length.
Claim 4
Dependent Claim
17 words
The composition of claim 1, wherein said polypeptide has a molecular weight of at least 50 kD.
Claim 5
Dependent Claim
11 words
The composition of claim 1, wherein said intervening sequence comprises RGD.
Claim 6
Dependent Claim
14 words
The composition of claim 4 wherein said protein polymer is ProNectin®F (SLPF) or SLPL3.0.
Claim 7
Dependent Claim
14 words
The composition of claim 1 wherein said thermoplastic is polystyrene, polyethylene, polypropylene, or polymethylmethacrylate.
Claim 8
Dependent Claim
12 words
The composition of claim 1 further comprising a polypeptide thermostability enhancing additive.
Claim 9
Dependent Claim
92 words
A formed object produced by contacting a plastic with a biologically functional thermostable polypeptide, heating said plastic and polypeptide to at least 60° C. for at least 15 seconds to create a melt, molding said melt to form a biologically functional thermostable polypeptide interdispersed within a thermoplastic, wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 10
Dependent Claim
92 words
A formed object produced by contacting a plastic with a biologically functional thermostable polypeptide, heating said plastic and polypeptide to at least 60° C. for at least 15 seconds to create a melt, molding said melt to form a biologically functional thermostable polypeptide interdispersed within a thermoplastic, wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 11
Independent Claim
98 words
A method for producing a biologically functional thermostable polypeptide interdispersed within a thermoplastic, said method comprising: forming a mixture of plastic and said thermostable polypeptide, heating said thermoplastic and said polypeptide to create a melt, molding said melt into a predetermined form, whereby a biologically functional thermostable polypeptide is interdispersed within a thermoplastic, wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 12
Independent Claim
98 words
A method for producing a biologically functional thermostable polypeptide interdispersed within a thermoplastic, said method comprising: forming a mixture of plastic and said thermostable polypeptide, heating said thermoplastic and said polypeptide to create a melt, molding said melt into a predetermined form, whereby a biologically functional thermostable polypeptide is interdispersed within a thermoplastic, wherein said polypeptide is at least about 25 kD, has repeating units from elastin, collagen, keratin or silk proteins, and sequences intervening said repeating units, said intervening sequences having chemically active amino acids or a naturally occurring sequence having binding specificity for a protein receptor.
Claim 13
Independent Claim
52 words
A method for producing biologically functional ProNectin®F (SLPF) interdispersed within a thermoplastic; said method comprising: forming a mixture of a thermoplastic and ProNectin®F (SLPF), heating said thermoplastic and said ProNectin®F (SLPF) to create a melt, molding said melt into a predetermined form, whereby biologically functional ProNectin®F (SLPF) is interdispersed within said thermoplastic.
Claim 14
Independent Claim
52 words
A method for producing biologically functional ProNectin®F (SLPF) interdispersed within a thermoplastic; said method comprising: forming a mixture of a thermoplastic and ProNectin®F (SLPF), heating said thermoplastic and said ProNectin®F (SLPF) to create a melt, molding said melt into a predetermined form, whereby biologically functional ProNectin®F (SLPF) is interdispersed within said thermoplastic.
References Cited (Backward Citations)
JPS57108010A
Cited
US4617271A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-03-02 | 2001-09-01 | 2002-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-02 | 2005-09-01 | 2006-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-02 | 2009-09-01 | 2010-03-03 | $7700.00 | Window Closed / Lapsed |