US5688692A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transgenic mouse cells expressing ts SV40 large T
Assignee: Ludwig Cancer Research
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Filing: 1993-02-11
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Granted: 1997-11-18
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Expires: 2013-02-11
Abstract
Abstract The provision of cell lines from virtually any cell type of the animal body is greatly facilitated by transgenic non-human eukaryotic animals of the invention in which at least some cells have (i) a differentiation inhibiting sequence chromosomally incorporated under the control of a non-constitutive promotor and/or (ii) a differentiation inhibiting sequence which is itself conditionally active. Said genes are chromosomally incorporated under the control of a promotor such that expression of said sequence is normally held below an effective level, thus allowing normal cell development. However, cells taken from said animal may be prevented from completing differentiation to a non-dividing state in tissue culture by activating expression of said sequence.
Inventors
- 👤 Parmjit Singh Jat
- 👤 Dimitris Kioussis
- 👤 Mark David Noble
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:01
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K2217/00; A01K2217/05; C; C12; C12N; C12N2510/00; C12N2510/04; C12N2517/00; C12N2517/02
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
95 words
Method for establishing a cell in culture comprising: (i) removing a cell from a transgenic mouse, wherein said cell has chromosomally integrated a DNA sequence encoding TAgts protein under the control of a non-constitutive promotor wherein expression of said TAgts protein has been at a sufficiently low functional level in vivo to permit normal development of said cell in said mouse, and (ii) subjecting the removed cell to permissive conditions in culture, wherein said permissive conditions lead to a functional level of expression of said TAgts protein sufficient to prevent complete differentiation of said cell.
Claim 2
Dependent Claim
95 words
Method for establishing a cell in culture comprising: (i) removing a cell from a transgenic mouse, wherein said cell has chromosomally integrated a DNA sequence encoding TAgts protein under the control of a non-constitutive promotor wherein expression of said TAgts protein has been at a sufficiently low functional level in vivo to permit normal development of said cell in said mouse, and (ii) subjecting the removed cell to permissive conditions in culture, wherein said permissive conditions lead to a functional level of expression of said TAgts protein sufficient to prevent complete differentiation of said cell.
Claim 3
Dependent Claim
15 words
Method of claim 1, wherein expression of said TAgts protein in culture immortalizes said cell.
Claim 4
Dependent Claim
17 words
Method of claim 1, wherein said promotor comprises a plurality of genetic regulatory elements in operable association.
Claim 5
Dependent Claim
16 words
Method of claim 1, wherein said promotor is a major histocompatability complex, class I antigen promotor.
Claim 6
Dependent Claim
12 words
Isolated cell obtained by the method of claim 1 after step (ii).
Claim 7
Dependent Claim
22 words
Cell line produced by culturing a cell isolated by the method of claim 1 after step (ii) under conditions favoring division thereof.
Claim 8
Dependent Claim
11 words
Method of claim 1, wherein said cell is a precursor cell.
Claim 9
Dependent Claim
39 words
Method of claim 1, wherein said cell is selected from the group consisting of a pancreatic cell, a precursor of an insulin producing cell, a glial cell, a glial cell precursor, a muscle cell, and a muscle cell precursor.
Claim 10
Dependent Claim
24 words
Method for producing an expression product of the cell of claim 5, comprising culturing said cell under conditions factoring production of said expression product.
Claim 11
Dependent Claim
26 words
Method for producing an expression product of the cell line of claim 6 comprising culturing said cell line under conditions favoring production of said expression product.
Claim 12
Dependent Claim
95 words
Method for establishing a cell in culture comprising: (i) removing a cell from a transgenic mouse, wherein said cell has chromosomally integrated a DNA sequence encoding TAgts protein under the control of a H-2K b promotor wherein expression of said TAgts protein is at a sufficiently low functional level in vivo to permit normal development of said cell in said mouse, and (ii) subjecting the removed cell to permissive conditions in culture, wherein said permissive conditions lead to a functional level of expression of said TAgts protein sufficient to prevent complete differentiation of said cell.
Claim 13
Dependent Claim
95 words
Method for establishing a cell in culture comprising: (i) removing a cell from a transgenic mouse, wherein said cell has chromosomally integrated a DNA sequence encoding TAgts protein under the control of a H-2K b promotor wherein expression of said TAgts protein is at a sufficiently low functional level in vivo to permit normal development of said cell in said mouse, and (ii) subjecting the removed cell to permissive conditions in culture, wherein said permissive conditions lead to a functional level of expression of said TAgts protein sufficient to prevent complete differentiation of said cell.
Claim 14
Dependent Claim
15 words
Method of claim 11, wherein expression of said TAgts protein in culture immortalizes said cell.
Claim 15
Dependent Claim
12 words
Isolated cell obtained by the method of claim 11 after step (ii).
Claim 16
Dependent Claim
22 words
Cell line produced by culturing a cell isolated by the method of claim 11 after step (ii) under conditions favoring division thereof.
Claim 17
Dependent Claim
11 words
Method of claim 11, wherein said cell is a precursor cell.
Claim 18
Dependent Claim
39 words
Method of claim 11, wherein said cell is selected from the group consisting of a pancreatic cell, a precursor of an insulin producing cell, a glial cell, a glial cell precursor, a muscle cell, and a muscle cell precursor.
Claim 19
Dependent Claim
24 words
Method for producing an expression product of the cell of claim 13 comprising culturing said cell under conditions favoring production of said expression product.
Claim 20
Dependent Claim
26 words
Method for producing an expression product of the cell line of claim 14 comprising culturing said cell line under conditions favoring production of said expression product.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2000-11-17 | 2001-05-19 | 2001-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-11-17 | 2005-05-19 | 2005-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-11-17 | 2009-05-19 | 2009-11-18 | $7700.00 | Window Closed / Lapsed |