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US5652116A
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Method for genetically manipulating peptide synthetases
Assignee: Eni Tecnologie SpA
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Filing: 1994-07-06
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Granted: 1997-07-29
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Expires: 2014-07-06
Abstract
Abstract Disclosed is a novel method for the in vivo and in vitro synthesis of modified peptides, using either engineered microorganisms capable of expressing peptide synthetases with modified substrate specificity, or peptide synthetases extracted from said microorganisms.
Inventors
- 👤 Guido Grandi
- 👤 Francesca De Ferra
- 👤 Giuliano Galli
- 👤 Paola Cosmina
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:33
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/93; C12P; C12P21/00; C12P21/02
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
60 words
A method for biosynthesizing a novel peptide which comprises: (a) constructing a gene encoding a peptide synthetase with an amino acid specificity different than any known peptide synthetase; (b) inserting said gene into a vector; (c) transforming a microorganism with said vector; (d) isolating transformants which express said peptide synthetase; and (e) cultivating said transformants to produce said novel peptide.
Claim 2
Independent Claim
60 words
A method for biosynthesizing a novel peptide which comprises: (a) constructing a gene encoding a peptide synthetase with an amino acid specificity different than any known peptide synthetase; (b) inserting said gene into a vector; (c) transforming a microorganism with said vector; (d) isolating transformants which express said peptide synthetase; and (e) cultivating said transformants to produce said novel peptide.
Claim 3
Dependent Claim
58 words
The method of claim 1, wherein in step (a), said gene is obtained by at least one step of: deleting one or more activation domain coding regions, substituting one or more activation domain coding regions from the same or different peptide synthetase, or adding one or more activation domain coding regions from the same or different peptide synthetase.
Claim 4
Dependent Claim
15 words
The method of claim 1, wherein said novel peptide has antibiotic, antifungal or immunosuppressive properties.
Claim 5
Dependent Claim
22 words
The method of claim 1, wherein said novel peptide comprises the same amino acid residues as surfactin but a different primary sequence.
Claim 6
Dependent Claim
12 words
The method of claim 1, wherein said vector is an expression plasmid.
Claim 7
Dependent Claim
12 words
The method of claim 1, wherein said vector is an integration plasmid.
Claim 8
Dependent Claim
14 words
The method of claim 1, wherein said microorganism is a bacterium, fungus or yeast.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-07-28 | 2001-01-27 | 2001-07-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-07-28 | 2005-01-27 | 2005-07-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-07-28 | 2009-01-27 | 2009-07-29 | $7700.00 | Window Closed / Lapsed |