Patent US5995441A successfully refreshed from Google Patents.
Patent US5651869A successfully refreshed from Google Patents.
US5651869A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor
Assignee: PHC Holdings Corp
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Filing: 1996-02-22
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Granted: 1997-07-29
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Expires: 2016-02-22
Abstract
Abstract The biosensor of this invention includes an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween. The reaction layer includes a pyranose-oxidizing enzyme.
Inventors
- 👤 Toshihiko Yoshioka
- 👤 Shin Ikeda
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 09:09
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/006; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
51 words
A biosensor comprising: an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween, wherein the reaction layer includes a pyranose-oxidizing enzyme and glucose oxidase (EC1.1.3.4).
Claim 2
Dependent Claim
51 words
A biosensor comprising: an electrically insulating substrate; an electrode system formed on the substrate which includes a working electrode and a counter electrode; and a reaction layer formed on the substrate or above the substrate with a space therebetween, wherein the reaction layer includes a pyranose-oxidizing enzyme and glucose oxidase (EC1.1.3.4).
Claim 3
Dependent Claim
14 words
A biosensor according to claim 1, wherein the pyranose-oxidizing enzyme is pyranose oxidase (EC1.1.3.10).
Claim 4
Dependent Claim
15 words
A biosensor according to claim 1, wherein the reaction layer further includes an electron acceptor.
Claim 5
Dependent Claim
14 words
A biosensor according to claim 3, wherein the electron acceptor is a ferricyanide ion.
Claim 6
Dependent Claim
48 words
A biosensor according to claim 4, wherein contents of the pyranose-oxidizing enzyme, the glucose oxidase, and the ferricyanide ion are about 0.1 to about 200 units, 1 to about 200 units, and about 0.21 mg to about 3.30 mg, respectively per 1 cm 2 of the reaction layer.
Claim 7
Dependent Claim
15 words
A biosensor according to claim 1, wherein the reaction layer further includes a polysaccharide hydrolase.
Claim 8
Dependent Claim
25 words
A biosensor according to claim 6, wherein the polysaccharide hydrolase is one selected from the group consisting of sucrose hydrolase, maltose hydrolase, and lactose hydrolase.
Claim 9
Dependent Claim
27 words
A biosensor according to claim 1, wherein a content of the pyranose-oxidizing enzyme is 1 to about 200 units per 1 cm 2 of the reaction layer.
Claim 10
Dependent Claim
28 words
A biosensor according to claim 1, wherein a content of the pyranose-oxidizing enzyme is about 0.1 to about 200 units per 1 cm 2 of the reaction layer.
Claim 11
Dependent Claim
17 words
A biosensor according to claim 1, wherein the biosensor is used for measuring a blood glucose level.
Claim 12
Dependent Claim
96 words
A biosensor for quantitating a biochemical substrate included in a sample liquid by reducing an electron acceptor with an electron generated through an enzyme reaction of the biochemical substrate and electrochemically measuring an amount of a reduced form of the electron acceptor, comprising: an electrically insulating substrate; an electrode system formed on the insulating substrate and including a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate or above the substrate with a space therebetween, wherein the reaction layer includes a pyranose-oxidizing enzyme, an electron acceptor, and glucose oxidase (EC1.1.3.4).
Claim 13
Dependent Claim
96 words
A biosensor for quantitating a biochemical substrate included in a sample liquid by reducing an electron acceptor with an electron generated through an enzyme reaction of the biochemical substrate and electrochemically measuring an amount of a reduced form of the electron acceptor, comprising: an electrically insulating substrate; an electrode system formed on the insulating substrate and including a working electrode and a counter electrode; and a reaction layer formed on the insulating substrate or above the substrate with a space therebetween, wherein the reaction layer includes a pyranose-oxidizing enzyme, an electron acceptor, and glucose oxidase (EC1.1.3.4).
Claim 14
Dependent Claim
13 words
A biosensor according to claim 11, wherein the biosensor is a glucose sensor.
Claim 15
Dependent Claim
17 words
A biosensor according to claim 11, wherein the biosensor is used for measuring a blood glucose level.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-07-28 | 2001-01-27 | 2001-07-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-07-28 | 2005-01-27 | 2005-07-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-07-28 | 2009-01-27 | 2009-07-29 | $7700.00 | Window Closed / Lapsed |