US5646008A
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Vertebrate apoptosis gene: compositions and methods
Assignee: Arch Development Corp
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Filing: 1993-06-22
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Granted: 1997-07-08
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Expires: 2013-06-22
Abstract
Abstract The invention relates generally to compositions of and methods for obtaining and using a polypeptide other than BCL-2 that affects programmed vertebrate cell death. The invention relates as well to polynucleotides encoding those polypeptides, recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant polypeptides. The invention further provides methods for using the isolated, recombinant polypeptides in assays designed to select and improve substances capable of altering programmed cell death for use in diagnostic, drug design and therapeutic applications.
Inventors
- 👤 Craig B. Thompson
- 👤 Lawrence H. Boise
- 👤 Gabriel Nunez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:37
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4747; A; A61; A61P; A61P35/00; A61P43/00; C07K14/465
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
10 words
An isolated and purified polynucleotide that encodes BCL-X L .
Claim 2
Dependent Claim
10 words
An isolated and purified polynucleotide that encodes BCL-X L .
Claim 3
Dependent Claim
15 words
The isolated and purified polynucleotide of claim 1, wherein said polynucleotide is a DNA molecule.
Claim 4
Dependent Claim
24 words
The DNA molecule of claim 2, wherein said encoded polypeptide comprises the amino acid residue sequence of SEQ ID NO:2 or SEQ ID NO:6.
Claim 5
Dependent Claim
24 words
The isolated and purified polynucleotide of claim 1, wherein said polynucleotide comprises the nucleotide base sequence of SEQ ID NO:1 or SEQ ID NO:5.
Claim 6
Dependent Claim
47 words
An isolated and purified polynueleotide comprising a base sequence that is identical or complementary to a segment of at least 25 contiguous bases of SEQ ID NO: 1 or SEQ ID NO:5, wherein said polynucleotide hybridizes to the sequence of SEQ ID NO:1 or SEQ ID NO:5.
Claim 7
Dependent Claim
47 words
An isolated and purified polynueleotide comprising a base sequence that is identical or complementary to a segment of at least 25 contiguous bases of SEQ ID NO: 1 or SEQ ID NO:5, wherein said polynucleotide hybridizes to the sequence of SEQ ID NO:1 or SEQ ID NO:5.
Claim 8
Dependent Claim
11 words
An expression vector comprising a polynucleotide that encodes BCL-X L .
Claim 9
Dependent Claim
11 words
An expression vector comprising a polynucleotide that encodes BCL-X L .
Claim 10
Dependent Claim
15 words
The expression vector of claim 6, wherein the polynucleotide is operatively linked to an enhancer-promoter.
Claim 11
Dependent Claim
13 words
A recombinant host cell transfected with a polynucleotide that encodes BCL-X L .
Claim 12
Dependent Claim
13 words
A recombinant host cell transfected with a polynucleotide that encodes BCL-X L .
Claim 13
Dependent Claim
45 words
The recombinant host cell of claim 8 where the polynucleotide is under the transcriptional control of regulatory signals functional in the recombinant host cell, wherein the regulatory signals appropriately control expression of said polypeptide in a manner to enable all necessary transcriptional and post-transcriptional modification.
Claim 14
Dependent Claim
46 words
A process of using a polynucleotide in accordance with claim 1 comprising: (a) transfecting a cell with a polynucleotide in accordance with claim 1 to produce a transformed host cell; and (b) maintaining the transformed host cell under biological conditions sufficient for expression of the polypeptide.
Claim 15
Dependent Claim
11 words
The polynucleotide of claim 1, further defined as a recombinant vector.
Claim 16
Dependent Claim
11 words
The polynucleotide of claim 5, further defined as a recombinant vector.
References Cited (Backward Citations)
US5082774A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-07-07 | 2001-01-06 | 2001-07-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-07-07 | 2005-01-06 | 2005-07-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-07-07 | 2009-01-06 | 2009-07-08 | $7700.00 | Window Closed / Lapsed |