US5635482A
Expired / Lapsed
US
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Synthetic compounds and compositions with enhanced cell binding
Assignee: CALIFORNIA A CORP OF CALIFORNIA, University of, Regents of
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Filing: 1994-07-22
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Granted: 1997-06-03
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Expires: 2014-07-22
Abstract
Abstract Compositions of the invention include composites comprising a biomaterial having compounds thereon with enhanced cell binding with respect to collagen. These composites are useful for soft and hard tissue repair or reconstruction and for in vitro uses. Suitable compounds with enhanced cell binding include synthetic peptides that mimic the conformation necessary for recognition and docking of collagen binding species (such as cell surface receptors for collagen and fibronectin) and have the amino acid residues -Ile-Ala- folded in a beta -bend.
Inventors
- 👤 Rajendra S. Bhatnagar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:04
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/28; A61L27/34; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; C12; C12N; C12N5/00; C12N5/0068; A61K; A61K38/00; A61L2430/00; A61L2430/02; C12N2533/00; C12N2533/30; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
116 words
An implant comprising: a matrix formed of a biomaterial and a peptide carried by the matrix, the peptide having enhanced cell binding with respect to collagen, the peptide having a domain that mimics collagen binding to cells, said domain including at least -Ile-Ala- folded in a β-bend at physiologic conditions, wherein the peptide has the sequence Gly-Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg-Gly-Val-Val (SEQ ID NO: 1), Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO: 2), Gln-Gly-Ile-Ala-Gly-Gln (SEQ ID NO: 3), Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO: 4), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO: 5), Gly-Ile-Ala-Gly-Gln (SEQ ID NO: 6), Gln-Gly-Ala-Ile-Ala-Gln (SEQ ID NO: 7), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:9), Cys-Gly-Ile-Ala-Gly-Cys (SEQ ID NO:10), Glu-Gly-Ile-Ala-Gly-Lys (SEQ ID NO:11), NAc-Ile-Ala-Ala (SEQ ID NO:12), Ile-Ala-βAla (SEQ ID NO:13), and NAc-Ile-Ala-N-Me (SEQ ID NO:14).
Claim 2
Dependent Claim
116 words
An implant comprising: a matrix formed of a biomaterial and a peptide carried by the matrix, the peptide having enhanced cell binding with respect to collagen, the peptide having a domain that mimics collagen binding to cells, said domain including at least -Ile-Ala- folded in a β-bend at physiologic conditions, wherein the peptide has the sequence Gly-Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg-Gly-Val-Val (SEQ ID NO: 1), Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO: 2), Gln-Gly-Ile-Ala-Gly-Gln (SEQ ID NO: 3), Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO: 4), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO: 5), Gly-Ile-Ala-Gly-Gln (SEQ ID NO: 6), Gln-Gly-Ala-Ile-Ala-Gln (SEQ ID NO: 7), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:9), Cys-Gly-Ile-Ala-Gly-Cys (SEQ ID NO:10), Glu-Gly-Ile-Ala-Gly-Lys (SEQ ID NO:11), NAc-Ile-Ala-Ala (SEQ ID NO:12), Ile-Ala-βAla (SEQ ID NO:13), and NAc-Ile-Ala-N-Me (SEQ ID NO:14).
Claim 3
Dependent Claim
26 words
The implant as in claim 1 wherein the peptide is in an amount effective to promote cell attachment, and the matrix has the peptide coated thereon.
Claim 4
Dependent Claim
20 words
The implant as in claim 2 wherein the matrix includes a ceramic, a metal, a polymer or a composite thereof.
Claim 5
Dependent Claim
14 words
The implant as in claim 1 or 2 wherein the matrix includes a hydrogel.
Claim 6
Dependent Claim
18 words
The implant as in claim 1 or 2 wherein the matrix is in the form of polymer fibers.
Claim 7
Dependent Claim
23 words
The implant as in claim 1 or 2 wherein at least part of the matrix is a soluble polymer or is a hydrogel.
Claim 8
Dependent Claim
14 words
The implant as in claim 1 wherein the peptide is bound to the matrix.
Claim 9
Dependent Claim
11 words
The implant as in claim 7 wherein the matrix has interstices.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-06-02 | 2000-12-02 | 2001-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-06-02 | 2004-12-02 | 2005-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-06-02 | 2008-12-02 | 2009-06-03 | $7700.00 | Window Closed / Lapsed |