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US5632273A
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Method and means for measurement of biochemical components
Assignee: Hamamatsu Photonics KK
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Filing: 1994-07-05
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Granted: 1997-05-27
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Expires: 2014-07-05
Abstract
Abstract Biochemical components and changes in the components are measured by directing light of several wavelengths into a body at one location, sensing light at the wavelengths emerging from the body at a plurality of distances from the one location, and ascertaining biochemical component characteristics in the body as a function of variations, with respect to distance, of the logarithm of the ratio of the light sensed to the light directed into the body. The variations with respect to distance are in the form of the derivative and square of the derivatives of the logarithm.
Inventors
- 👤 Susumu Suzuki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:03
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/145; A61B5/1455; A61B5/0059; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/314; G01N21/47; G01N21/49
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
79 words
The method, comprising: directing electromagnetic radiation at one or more wavelengths into a body at one location; sensing electromagnetic radiation at the wavelengths emerging from the body at a plurality of distances from the one location; and ascertaining biochemical component characteristics in the body as a function of differences in a negative logarithm of the electromagnetic radiation sensed for the wavelengths at the plurality of distances, and a scattering coefficient relative to the wavelengths at each of the distances.
Claim 2
Independent Claim
79 words
The method, comprising: directing electromagnetic radiation at one or more wavelengths into a body at one location; sensing electromagnetic radiation at the wavelengths emerging from the body at a plurality of distances from the one location; and ascertaining biochemical component characteristics in the body as a function of differences in a negative logarithm of the electromagnetic radiation sensed for the wavelengths at the plurality of distances, and a scattering coefficient relative to the wavelengths at each of the distances.
Claim 3
Dependent Claim
24 words
The method as in claim 1, wherein the differences with respect to distance are in a form of the derivative of the negative logarithm.
Claim 4
Dependent Claim
32 words
The method as in claim 2, wherein the step of ascertaining further includes determining concentrations of biochemical components as a function of the square of said derivative, and a molar extinction coefficient.
Claim 5
Dependent Claim
86 words
The method as in claim 3, wherein the sensing is performed on a number of elements j by a plurality of detectors each at one of the plurality of distances, and wherein the step of determining includes calculating a concentration C j each of the elements j on the basis of equation ##EQU22## and OD=-log(I o /I i ), μ a is an extinction coefficient, μ' s is a scattering coefficient, and ε j is a known molar extinction coefficient for each of the elements j.
Claim 6
Dependent Claim
92 words
The method as in claim 3, wherein the sensing is performed on a number of elements j by a plurality of detectors each at one of the plurality of distances, and wherein the step of determining includes calculating a concentration C j for each of the elements j on the basis of equation ##EQU23## and OD=-log(I o /I i ), μ a =an extinction coefficient, μ a is an extinction coefficient, μ' s is a scattering coefficient, and ε j is a known molar extinction coefficient for each of the elements j.
Claim 7
Dependent Claim
42 words
The method as in claim 5, wherein the step of calculating includes using the matrix formula ##EQU24## where μ ai is for the wavelength λi and N is at least equal to a number of compounds determined plus 1 for a baseline.
Claim 8
Dependent Claim
18 words
The method as in claim 3, wherein the step of determining includes calculating concentrations from the matrix ##EQU25##
Claim 9
Dependent Claim
27 words
The method as in claim 3, wherein the step of determining includes calculating concentrations from the matrix ##EQU26## and calculating changes in concentration from the matrix ##EQU27##
Claim 10
Dependent Claim
42 words
The method as in claim 2, wherein the step of ascertaining further includes determining changes, with respect to time, of concentrations of biochemical components as a function of changes in time of the square of said derivative, and a molar extinction coefficient.
Claim 11
Dependent Claim
20 words
The method as in claim 9, wherein the step of determining includes calculating changes in concentrations from the matrix ##EQU28##
Claim 12
Dependent Claim
27 words
The method as in claim 1, wherein the step of ascertaining further includes determining concentrations of biochemical components as a function of the square of said differences.
Claim 13
Dependent Claim
32 words
The method as in claim 1, wherein the step of ascertaining further includes determining concentrations of biochemical components as a function of the square of said differences and a molar extinction coefficient.
Claim 14
Dependent Claim
42 words
The method as in claim 1, wherein the step of ascertaining further includes determining changes, with respect to time, of concentrations of biochemical components as a function of changes in time of the square of said differences and a molar extinction coefficient.
Claim 15
Dependent Claim
24 words
The method as in claim 1, wherein the step of directing directs the radiation at each wavelength in an impulse or sine wave form.
Claim 16
Independent Claim
87 words
An apparatus, comprising: means for directing electromagnetic radiation at one or more wavelengths into a body at one location; means for sensing electromagnetic radiation at the plurality of wavelengths emerging from the body at a plurality of distances from the one location; and means for ascertaining biochemical component characteristics in the body as a function of differences in a negative logarithm of the electromagnetic radiation sensed for the wavelengths at the plurality of distances, and a scattering coefficient relative to the wavelengths at each of the distances.
Claim 17
Independent Claim
87 words
An apparatus, comprising: means for directing electromagnetic radiation at one or more wavelengths into a body at one location; means for sensing electromagnetic radiation at the plurality of wavelengths emerging from the body at a plurality of distances from the one location; and means for ascertaining biochemical component characteristics in the body as a function of differences in a negative logarithm of the electromagnetic radiation sensed for the wavelengths at the plurality of distances, and a scattering coefficient relative to the wavelengths at each of the distances.
Claim 18
Dependent Claim
32 words
The apparatus as in claim 15, wherein, in the means for ascertaining, the differences with respect to distance are in the form of a derivative of the negative logarithm for the wavelengths.
Claim 19
Dependent Claim
33 words
The apparatus as in claim 16, wherein the means for ascertaining includes means for determining concentrations of biochemical components as a function of the square of said derivative, and a molar extinction coefficient.
Claim 20
Dependent Claim
86 words
The apparatus as in claim 17, wherein the means for sensing includes a plurality of detectors each for one of elements j and each at one of the distances, and wherein the means for determining includes means for calculating a concentration c j for each of the elements j on the basis of equation ##EQU29## where OD=-log(I o /I i ), μ a is an extinction coefficient, μ' s is a scattering coefficient, and ε j is a known molar extinction coefficient of the element j.
Claim 21
Dependent Claim
88 words
The apparatus as in claim 17, wherein the means for sensing includes a plurality of detectors each for one of elements j and each at one of the distances, and wherein the means for determining includes means for calculating a concentration C j for each of the elements j on the basis of equation ##EQU30## and OD=-log(I o /I i ), ε j is a known molar extinction coefficient for each of the elements j, μ a is an extinction coefficient, and μ' s is a scattering coefficient.
Claim 22
Dependent Claim
44 words
The apparatus as in claim 19, wherein the means for calculating includes means for executing the matrix formula ##EQU31## where μ ai is for the wavelength λi and N is at least equal to the number of compounds determined plus 1 for a baseline.
Claim 23
Dependent Claim
20 words
The apparatus as in claim 17, wherein the means for determining includes means for calculating concentrations from the matrix ##EQU32##
Claim 24
Dependent Claim
31 words
The apparatus as in claim 17, wherein the means for determining includes means for calculating concentrations from the matrix ##EQU33## and means for calculating changes in concentration from the matrix ##EQU34##
Claim 25
Dependent Claim
38 words
The apparatus as in claim 16, wherein the means for ascertaining further includes means for determining changes in concentrations of biochemical components as a function of changes in the square of said derivative, and a molar extinction coefficient.
Claim 26
Dependent Claim
22 words
The apparatus as in claim 23, wherein the means for determining includes means for calculating changes in concentrations from the matrix ##EQU35##
Claim 27
Dependent Claim
29 words
The apparatus as in claim 15, wherein the means for ascertaining further includes means for determining concentrations of biochemical components as a function of the square of said differences.
Claim 28
Dependent Claim
34 words
The apparatus as in claim 15, wherein the means for ascertaining further includes means for determining concentrations of biochemical components as a function of the square of said differences and a molar extinction coefficient.
Claim 29
Dependent Claim
36 words
The apparatus as in claim 15, wherein the means for ascertaining further includes means for determining differences in concentrations of biochemical components as a function of the square of said differences and a molar extinction coefficient.
Claim 30
Dependent Claim
24 words
An apparatus as in claim 15, wherein said directing means directs radiation at each of the wavelengths in a pulse or sine wave form.
References Cited (Backward Citations)
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