Patent US5627053A successfully refreshed from Google Patents.
US5627053A
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2'deoxy-2'-alkylnucleotide containing nucleic acid
Assignee: Sirna Therapeutics Inc
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Filing: 1995-05-02
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Granted: 1997-05-06
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Expires: 2015-05-02
Abstract
Abstract 2'-deoxy-2'-alkylnucleotides useful for stabilizing enzymatic nucleic acid molecules and antisense molecules.
Inventors
- 👤 Nassim Usman
- 👤 Alexander Karpeisky
- 👤 Leonid Beigelman
- 👤 Anil Modak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:01
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H19/16; C07H19/20; C07H21/00; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101; C12N15/1136; C12N15/1137; C12N15/1138; C12Y; C12Y304/00; C12Y304/24; C12Y304/24017; A; A61; A61K; A61K38/00; A61K48/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121; C12N2310/122; C12N2310/123; C12N2310/124; C12N2310/1241; C12N2310/126; C12N2310/127; C12N2310/30; C12N2310/31; C12N2310/315; C12N2310/32; C12N2310/321; C12N2310/322; C12N2310/33; C12N2310/332; C12N2310/333; C12N2310/334; C12N2310/335; C12N2310/336; C12N2310/34; C12N2310/346; C12N2310/35; C12N2310/351; C12N2310/3513; C12N2310/352; C12N2310/3523; C12N2310/3527; C12N2310/353; C12N2310/3533; C12N2310/3535
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
6 words
Oligonucleotide comprising one or more 2'-deoxy-2'-alkyl-nucleotides.
Claim 2
Dependent Claim
6 words
Oligonucleotide comprising one or more 2'-deoxy-2'-alkyl-nucleotides.
Claim 3
Dependent Claim
13 words
Enzymatic nucleic acid Capable of cleaving single-stranded RNA or DNA comprising a 2'-deoxy-2'-alkylnucleotide.
Claim 4
Dependent Claim
13 words
Enzymatic nucleic acid Capable of cleaving single-stranded RNA or DNA comprising a 2'-deoxy-2'-alkylnucleotide.
Claim 5
Dependent Claim
40 words
Method for producing an enzymatic nucleic acid molecule having enzymatic activity capable of cleaving a single-stranded RNA or DNA molecule, comprising the step of forming said enzymatic molecule with at least one nucleotide having at its 2'-position an alkyl group.
Claim 6
Dependent Claim
40 words
Method for producing an enzymatic nucleic acid molecule having enzymatic activity capable of cleaving a single-stranded RNA or DNA molecule, comprising the step of forming said enzymatic molecule with at least one nucleotide having at its 2'-position an alkyl group.
Claim 7
Dependent Claim
2 words
2'-deoxy-2'-alkylnucleotide triphosphate.
Claim 8
Dependent Claim
2 words
2'-deoxy-2'-alkylnucleotide triphosphate.
Claim 9
Dependent Claim
63 words
Method for synthesis of a 2'-C-allyl derivative of a nucleoside from a 5'-O-dimethoxytrityl-3 '-O-t-bulydimethylsilyl-base comprising the steps of: (a) Phenoxythiocarbonylation of 5'O-dimethoxytrityl-3'-O-t-bulydimethylsilyl-base to yield a thioester, replacing a 2' hydroxyl group with a phenoxythiocarbonyl groups, and (b) Heck allylation of said thioester to form a 2'-C-allyl derivative in which said 2'-phenoxythiocarbonyl group is replaced with a 2'-allyl group to yield said 2'-C-allyl derivative.
Claim 10
Dependent Claim
63 words
Method for synthesis of a 2'-C-allyl derivative of a nucleoside from a 5'-O-dimethoxytrityl-3 '-O-t-bulydimethylsilyl-base comprising the steps of: (a) Phenoxythiocarbonylation of 5'O-dimethoxytrityl-3'-O-t-bulydimethylsilyl-base to yield a thioester, replacing a 2' hydroxyl group with a phenoxythiocarbonyl groups, and (b) Heck allylation of said thioester to form a 2'-C-allyl derivative in which said 2'-phenoxythiocarbonyl group is replaced with a 2'-allyl group to yield said 2'-C-allyl derivative.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-05-05 | 2000-11-04 | 2001-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-05-05 | 2004-11-04 | 2005-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-05-05 | 2008-11-04 | 2009-05-06 | $7700.00 | Window Closed / Lapsed |