Patent US5624711A successfully refreshed from Google Patents.
US5624711A
Expired / Lapsed
US
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Derivatization of solid supports and methods for oligomer synthesis
Assignee: Affymetrix Inc
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Filing: 1995-04-27
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Granted: 1997-04-29
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Expires: 2015-04-27
Abstract
Abstract Methods and derivatized supports which are useful in solid-phase synthesis of peptides, oligonucleotides or other small organic molecules as well as arrays of ligands. The methods provide means to control the functional site density on a solid support. Some of the derivatized supports are polymer-coated or glycan-coated. Other methods for regenerating the surface of a used ligand array are also provided.
Inventors
- 👤 Steven A. Sundberg
- 👤 David Fujimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:56
CPC Classifications
B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y30/00; B05; B05D; B05D1/00; B05D1/18; B05D1/185; B82Y40/00; C; C03; C03C; C03C17/00; C03C17/28; C03C17/30; C03C17/34; C03C17/3405; C40; C40B; C40B80/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00364; B01J2219/00378; B01J2219/00427; B01J2219/0043; B01J2219/00497; B01J2219/005; B01J2219/00504; B01J2219/00527; B01J2219/00529; B01J2219/00583; B01J2219/0059; B01J2219/00592; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00619; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00639; B01J2219/00641; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
158 words
A method for affixing functional sites to the surface of a solid substrate, comprising: (a) contacting said solid substrate with a derivatizaton reagent, said reagent having a substrate attaching group and a reactive site, to covalently bind said substrate attaching group to said substrate and thereby form a derivatized substrate having reactive sites; and (b) contacting said derivatized substrate with a mixture comprising linking molecules and diluent molecules, each of said linking molecules and diluent molecules having reactive groups capable of covalently binding to said reactive sites and said linking molecules alone further having a functional site for synthesis initiation, wherein said linking molecules and said diluent molecules are different and have different reactive properties and the ratio of said linking molecules to said diluent molecules in said mixture is selected to control the functional site density on said surface, to thereby bind said linking molecules and said diluent molecules to said substrate in accordance with said ratio.
Claim 2
Independent Claim
158 words
A method for affixing functional sites to the surface of a solid substrate, comprising: (a) contacting said solid substrate with a derivatizaton reagent, said reagent having a substrate attaching group and a reactive site, to covalently bind said substrate attaching group to said substrate and thereby form a derivatized substrate having reactive sites; and (b) contacting said derivatized substrate with a mixture comprising linking molecules and diluent molecules, each of said linking molecules and diluent molecules having reactive groups capable of covalently binding to said reactive sites and said linking molecules alone further having a functional site for synthesis initiation, wherein said linking molecules and said diluent molecules are different and have different reactive properties and the ratio of said linking molecules to said diluent molecules in said mixture is selected to control the functional site density on said surface, to thereby bind said linking molecules and said diluent molecules to said substrate in accordance with said ratio.
Claim 3
Dependent Claim
28 words
A method in accordance with claim 1, wherein said substrate attaching group is a member selected from the group consisting of a trialkoxysilyl radical and a trichlorosilyl radical.
Claim 4
Dependent Claim
30 words
A method in accordance with claim 1, wherein said reactive site is a member selected from the group consisting of amino, hydroxyl, carboxylic acid, thiol, ester, amide, isocyanate and isothiocyanate.
Claim 5
Dependent Claim
14 words
A method in accordance with claim 1, wherein said derivatization reagent is an aminoalkyltrialkoxysilane.
Claim 6
Dependent Claim
30 words
A method in accordance with claim 1, wherein said functional site is a member selected from the group consisting of amino, hydroxyl, carboxylic acid, thiol, ester, amide, isocyanate and isothiocyanate.
Claim 7
Dependent Claim
15 words
A method in accordance with claim 1, wherein said diluent molecules are protected amino acids.
Claim 8
Dependent Claim
37 words
A method in accordance with claim 1, wherein said diluent molecules are members selected from the group consisting of protected glycine, protected serine and protected lysine, protected glutamic acid, protected aspartic acid, protected ornithine and protected phenylalanine.
Claim 9
Dependent Claim
17 words
A method in accordance with claim 1, wherein said ratio is from about 1:2 to about 1:200.
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| 3.5 Years (Year 4) | 2000-04-28 | 2000-10-28 | 2001-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-28 | 2004-10-28 | 2005-04-29 | $3760.00 | Window Closed / Lapsed |
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