Patent US3989174A successfully refreshed from Google Patents.
Patent US5618692A successfully refreshed from Google Patents.
US5618692A
Expired / Lapsed
US
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Zwittermicin resistance gene and biocontrol bacteria with the gene
Assignee: Wisconsin Alumni Research Foundation
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Filing: 1994-09-12
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Granted: 1997-04-08
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Expires: 2014-09-12
Abstract
Abstract A new gene from Bacillus cereus has been characterized which codes for a protein conferring resistance to the antibiotic zwittermicin A produced by some strains of the bacteria. The resistance gene, designated zmaR, is found to be present in almost all strains of the bacteria which are known to natively have the ability to synthesize zwittermicin A, and thus allows tests for the presence of the gene to serve as a test to be used with new strains to assay for zwittermicin A production. The gene also has use in increasing production of the antibiotic by producing strains.
Inventors
- 👤 Jo Handelsman
- 👤 Jocelyn L. Milner
- 👤 Elizabeth A. Stohl
- 👤 Sandra J. Stewart
- 👤 Eric Stabb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 19:50
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12P13/00; C12P13/02; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/085
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
42 words
A novel DNA construction isolated from its native host comprising a nucleotide sequence which can be expressed to encode a Bacillus cereus protein which, when present in a bacterial host cell, confers resistance in the host cell to the antibiotic zwittermicin A.
Claim 2
Dependent Claim
42 words
A novel DNA construction isolated from its native host comprising a nucleotide sequence which can be expressed to encode a Bacillus cereus protein which, when present in a bacterial host cell, confers resistance in the host cell to the antibiotic zwittermicin A.
Claim 3
Dependent Claim
24 words
A DNA sequence isolated from any host comprising a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 2.
Claim 4
Dependent Claim
24 words
A DNA sequence isolated from any host comprising a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 2.
Claim 5
Dependent Claim
16 words
A DNA construction isolated from Bacillus cereus comprising the DNA sequence of SEQ ID NO: 1.
Claim 6
Dependent Claim
16 words
A DNA construction isolated from Bacillus cereus comprising the DNA sequence of SEQ ID NO: 1.
Claim 7
Dependent Claim
16 words
A novel DNA construction isolated from its native host comprising the zmaR gene from Bacillus cereus.
Claim 8
Dependent Claim
16 words
A novel DNA construction isolated from its native host comprising the zmaR gene from Bacillus cereus.
Claim 9
Dependent Claim
21 words
An isolated DNA sequence comprising a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 2.
Claim 10
Dependent Claim
21 words
An isolated DNA sequence comprising a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 2.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-04-07 | 2000-10-07 | 2001-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-07 | 2004-10-07 | 2005-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-04-07 | 2008-10-07 | 2009-04-08 | $7700.00 | Window Closed / Lapsed |