Spectral measuring method and spectral measuring apparatus

Claim 2

A spectral measuring method comprising steps of: dividing excitation light of a single wavelength into a sample beam and a correction beam; irradiating a sample with said sample beam; selectively receiving at least either fluorescence or Raman scattered light as target light from light being obtained from said sample by said irradiation with said sample beam after removing the same wavelength component as said excitation light; simultaneously introducing received said target light and said correction beam into a single spectroscope as a single light beam and separating the light beam into spectral components thereby obtaining a spectrum; finding a spectral intensity at a prescribed wavelength or an integral value in a proper wavelength range from said spectrum as a measured value; and correcting said measured value on the basis of a detected intensity of an excitation light component in said spectrum.

Claim 3

A spectral measuring apparatus comprising: an excitation light source part being provided with a light source and a beam splitter for dividing a excitation light beam being obtained from said excitation light source into a sample beam and a correction beam; a sample part for irradiating a sample with said sample beam; an optical target adjusting part being provided with filter means for removing the same wavelength component as excitation light from light being generated from said sample being irradiated with said sample beam and selecting at least either fluorescence or Raman scattered light as target light and an optical system for adjusting beams; a beam combining means for placing a beam outgoing from said optical target adjusting part and said correction beam on the same optical axis; a spectral processing part being provided with a spectroscope for separating a beam outgoing from said beam combining means into its spectral components and a detector for detecting said spectral components being separated by said spectroscope; and a data processing part having functions of finding a spectral intensity at a prescribed wavelength or an integral value in a proper wavelength range from a spectrum being detected by said detector of said spectral processing part as a measured value and correcting said measured value on the basis of a detected intensity of an excitation light component in said spectrum.

Claim 4

A spectral measuring apparatus comprising: an excitation light source part being provided with a light source and a beam splitter for dividing a excitation light beam being obtained from said excitation light source into a sample beam and a correction beam; a sample part for irradiating a sample with said sample beam; an optical target adjusting part being provided with filter means for removing the same wavelength component as excitation light from light being generated from said sample being irradiated with said sample beam and selecting at least either fluorescence or Raman scattered light as target light and an optical system for adjusting beams; a beam combining means for placing a beam outgoing from said optical target adjusting part and said correction beam on the same optical axis; a spectral processing part being provided with a spectroscope for separating a beam outgoing from said beam combining means into its spectral components and a detector for detecting said spectral components being separated by said spectroscope; and a data processing part having functions of finding a spectral intensity at a prescribed wavelength or an integral value in a proper wavelength range from a spectrum being detected by said detector of said spectral processing part as a measured value and correcting said measured value on the basis of a detected intensity of an excitation light component in said spectrum.

Claim 5

The spectral measuring apparatus in accordance with claim 2, wherein said light source is a laser unit.

Claim 6

The spectral measuring apparatus in accordance with claim 2, wherein said light source is a lamp combined with a spectroscope.

Claim 7

The spectral measuring apparatus in accordance with claim 2, wherein said filter means in said optical target adjusting part is a holographic notch filter including an excitation light wavelength in its notch region.

Claim 8

The spectral measuring apparatus in accordance with claim 2, wherein said filter means in said optical target adjusting part is a cut filter shielding an excitation light wavelength and a shorter wavelength side therefrom.

Claim 9

The spectral measuring apparatus in accordance with claim 2, wherein said filter means in said optical target adjusting part is a bandpass filter having characteristics of transmitting to remove an excitation light wavelength component while reflecting other wavelength components.

Claim 10

The spectral measuring apparatus in accordance with claim 2, wherein said filter means in said optical target adjusting part is a holographic beam splitter removing an excitation light wavelength by transmission or reflection.

Claim 11

The spectral measuring apparatus in accordance with claim 2, wherein said spectral processing part is a polychrometer comprising a multi-channel photodetector for simultaneously detecting wavelength regions to be measured.

Claim 12

The spectral measuring apparatus in accordance with claim 2, wherein said spectral processing part is a dispersive and scanning type spectroscope.