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US5605829A
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Stroma-derived stem cell proteoglycan growth factor
Assignee: University of Minnesota System
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Filing: 1994-11-30
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Granted: 1997-02-25
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Expires: 2014-11-30
Abstract
Abstract An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD.
Inventors
- 👤 Philip B. McGlave
- 👤 Catherine M. Verfaillie
- 👤 Pankaj Gupta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 19:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4725; C07K14/76; C07K14/77; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0647; C12N2501/00; C12N2501/10; C12N2501/125; C12N2501/20; C12N2501/22; C12N2501/23; C12N2501/2306; C12N2501/235; C12N2501/90; C12N2501/998; C12N2502/00; C12N2502/13; C12N2502/1394
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
59 words
A synthetic proteoglycan, having a core protein and a polysaccharide portion, that is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or a combination thereof, and the core protein comprises ovalbumin.
Claim 2
Dependent Claim
59 words
A synthetic proteoglycan, having a core protein and a polysaccharide portion, that is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or a combination thereof, and the core protein comprises ovalbumin.
Claim 3
Dependent Claim
22 words
The composition of claim 1 wherein the polysaccharide portion and the core protein of the synthetic proteoglycan are linked by 1-ethyl-3(-3-dimethylaminopropyl)-carbodiimide coupling.
Claim 4
Independent Claim
88 words
A method for culturing hematopoietic cells, comprising: culturing a population of hematopoietic cells comprising stem cells or committed progenitor cells in a culture medium comprising a synthetic proteoglycan having a core protein and a polysaccharide portion, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or combinations thereof and the core protein comprises ovalbumin, so that the ability of the stem cell population to differentiate and self-replicate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Claim 5
Independent Claim
88 words
A method for culturing hematopoietic cells, comprising: culturing a population of hematopoietic cells comprising stem cells or committed progenitor cells in a culture medium comprising a synthetic proteoglycan having a core protein and a polysaccharide portion, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or combinations thereof and the core protein comprises ovalbumin, so that the ability of the stem cell population to differentiate and self-replicate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Claim 6
Dependent Claim
15 words
The method of claim 3 wherein the hematopoietic cells consist essentially of committed progenitor cells.
Claim 7
Dependent Claim
12 words
The method of claim 3 wherein the hematopoietic cells comprise stem cells.
Claim 8
Dependent Claim
15 words
The method of claim 3 wherein the hematopoietic cells are derived from bone marrow cells.
Claim 9
Dependent Claim
22 words
The method of claim 3 wherein the hematopoietic cells are isolated from adult peripheral blood, fetal liver blood or newborn cord blood.
Claim 10
Dependent Claim
11 words
The method of claim 5 wherein the stem cells are CD34+.
Claim 11
Dependent Claim
11 words
The method of claim 8 wherein the stem cells are Lin-CD34+DR-.
Claim 12
Dependent Claim
17 words
The method of claim 3 wherein the hematopoietic cells are human stromal cells or murine stromal cells.
Claim 13
Dependent Claim
23 words
The method of claim 3 wherein the cell culture medium comprises an effective stimulatory amount of IL-3, macrophage inflammatory protein-1α or mixtures thereof.
Claim 14
Dependent Claim
10 words
The method of claim 3 wherein the population is allogenic.
Claim 15
Dependent Claim
10 words
The method of claim 3 wherein the population is autologous.
Claim 16
Dependent Claim
16 words
The method of claim 3 wherein the hematopoietic cell population is supported on a microporous membrane.
Claim 17
Dependent Claim
29 words
The method of claim 14 wherein the hematopoietic cell population is supported on a microporous membrane, wherein said membrane allows the culture medium to contact the hematopoietic cell population.
Claim 18
Dependent Claim
20 words
The method of claim 15 wherein 25-100% of the culture medium is exchanged at intervals of about 8 hours-14 days.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-02-25 | 2000-08-26 | 2001-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-02-25 | 2004-08-26 | 2005-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-02-25 | 2008-08-26 | 2009-02-25 | $7700.00 | Window Closed / Lapsed |