Patent US5593874A successfully refreshed from Google Patents.
US5593874A
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Enhanced expression in plants
Assignee: Monsanto Technology LLC
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Filing: 1992-03-19
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Granted: 1997-01-14
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Expires: 2012-03-19
Abstract
Abstract This invention provides HSP70 introns that when present in a non-translated leader of a chimeric gene enhance expression in plants.
Inventors
- 👤 Sherri M. Brown
- 👤 Colleen G. Santino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:18
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C07; C07K; C07K14/00; C07K14/415
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
55 words
In a method for the expression of a chimeric plant gene in monocot plants, the improvement which comprises expressing a chimeric plant gene comprising an intron selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 in the non-translated leader 5' of the structural DNA sequence encoding a protein.
Claim 2
Dependent Claim
55 words
In a method for the expression of a chimeric plant gene in monocot plants, the improvement which comprises expressing a chimeric plant gene comprising an intron selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 in the non-translated leader 5' of the structural DNA sequence encoding a protein.
Claim 3
Dependent Claim
13 words
The method of claim 1 in which the intron is SEQ ID NO:1.
Claim 4
Dependent Claim
13 words
The method of claim 1 in which the intron is SEQ ID NO:2.
Claim 5
Dependent Claim
13 words
The method of claim 1 in which the intron is SEQ ID NO:3.
Claim 6
Dependent Claim
15 words
The method of claim 2 in which the structural DNA sequence encodes a EPSP synthase.
Claim 7
Dependent Claim
13 words
The method of claim 2 in which the structural DNA sequence encodes ACC-deaminase.
Claim 8
Dependent Claim
15 words
The method of claim 2 in which the structural DNA sequence encodes a GOX protein.
Claim 9
Dependent Claim
17 words
The method of claim 2 in which the structural DNA sequence encodes a B.t. crystal toxin protein.
Claim 10
Dependent Claim
15 words
The method of claim 3 in which the structural DNA sequence encodes a glgC16 protein.
Claim 11
Dependent Claim
103 words
A transgenic monocot plant comprising a DNA construct comprising in sequence: (a) a promoter that functions in plant cells to cause the production of an RNA sequence; (b) a non-translated leader comprising an intron sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3; (c) a structural DNA sequence that causes the production of an RNA sequence that encodes a protein; and (d) a 3' nontranslated sequence that functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence; the intron being heterologous with respect to the promoter.
Claim 12
Dependent Claim
103 words
A transgenic monocot plant comprising a DNA construct comprising in sequence: (a) a promoter that functions in plant cells to cause the production of an RNA sequence; (b) a non-translated leader comprising an intron sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3; (c) a structural DNA sequence that causes the production of an RNA sequence that encodes a protein; and (d) a 3' nontranslated sequence that functions in plant cells to cause the addition of polyadenylated nucleotides to the 3' end of the RNA sequence; the intron being heterologous with respect to the promoter.
Claim 13
Dependent Claim
16 words
The plant of claim 10 in which the intron is the sequence of SEQ ID NO:1.
Claim 14
Dependent Claim
16 words
The plant of claim 10 in which the intron is the sequence of SEQ ID NO:2.
Claim 15
Dependent Claim
16 words
The plant of claim 10 in which the intron is the sequence of SEQ ID NO:3.
Claim 16
Dependent Claim
11 words
The plant of claim 11 in which the plant is maize.
Claim 17
Dependent Claim
11 words
The plant of claim 11 in which the plant is wheat.
Claim 18
Dependent Claim
11 words
The plant of claim 11 in which the plant is rice.
Claim 19
Dependent Claim
23 words
The plant of claim 11 in which the promoter is selected from the group consisting of a CaMV35S promoter and a FMV promoter.
Claim 20
Dependent Claim
15 words
The plant of claim 17 wherein the structural DNA sequence encodes for an EPSP synthase.
Claim 21
Dependent Claim
15 words
The plant of claim 11 wherein the structural DNA sequence encodes for a CP4 protein.
Claim 22
Dependent Claim
13 words
The plant of claim 11 wherein the structural DNA sequence encodes for ACC-deaminase.
Claim 23
Dependent Claim
17 words
The plant of claim 11 wherein the structural DNA sequence encodes for a B.t. crystal toxin protein.
Claim 24
Dependent Claim
14 words
The plant of claim 17 wherein the structual DNA sequence encodes for glgC16 protein.
Claim 25
Dependent Claim
17 words
The plant of claim 11 wherein the structual DNA sequence encodes for a plant viral coat protein.
References Cited (Backward Citations)
No citation records indexed.
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