US5585116A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of manufacture of a material for osteoplasty from a natural bone tissue and material obtained thereby
Assignee: OST-DEVELOPPEMENT
•
Filing: 1995-02-03
•
Granted: 1996-12-17
•
Expires: 2015-02-03
Abstract
Abstract Osteoplasty material substantially devoid of antigenic proteins, wherein the material contains a natural bone structure in which non-denatured type I collagen is integrated, is prepared by selective extraction of bone tissue of human or animal origin. The selective extractant is an aqueous solution of 2-10 molar urea, and the extraction step is conducted at preferably 10°-30° C.
Inventors
- 👤 Robert Boniface
- 👤 Michel Faurie
- 👤 Pablo Goldschmidt
- 👤 Jean-Pierre Lontrade
- 👤 Jacques Luyckx
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:50
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L27/3608; A61F; A61F2/00; A61F2/02; A61F2/28; A61L27/14; A61L27/22; A61L27/24; A61L27/3641; A61L27/3645; A61L27/365; A61L27/3683; A61L27/3687; A61L27/3691; A61F2310/00; A61F2310/00005; A61F2310/00365
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
102 words
A method for producing an osteoplasty material, comprising (a) providing a starting non-demineralized bone tissue of human or animal origin, containing antigenic, non-collagenous structural proteins and structural collagen, (b) subjecting said bone tissue to at least one selective extraction of the antigenic proteins with an aqueous selective extraction agent containing urea at a sufficient concentration and a sufficient temperature so as to selectively decompose the antigenic non-collagenous protein structure without attacking type I collagen, and (c) recovering an osteoplasty material for bone implants having the natural bone structure of the starting bone tissue and type I collagen integrated therein in non-denatured form.
Claim 2
Independent Claim
102 words
A method for producing an osteoplasty material, comprising (a) providing a starting non-demineralized bone tissue of human or animal origin, containing antigenic, non-collagenous structural proteins and structural collagen, (b) subjecting said bone tissue to at least one selective extraction of the antigenic proteins with an aqueous selective extraction agent containing urea at a sufficient concentration and a sufficient temperature so as to selectively decompose the antigenic non-collagenous protein structure without attacking type I collagen, and (c) recovering an osteoplasty material for bone implants having the natural bone structure of the starting bone tissue and type I collagen integrated therein in non-denatured form.
Claim 3
Dependent Claim
26 words
The method of claim 1, wherein the urea is in the form of an aqueous solution at a concentration within the range of 2 to 10M.
Claim 4
Dependent Claim
15 words
The method of claim 2, wherein the selective extraction step is conducted at 10°-30° C.
Claim 5
Dependent Claim
14 words
The method of claim 2, wherein the concentration of the urea is 5-8 molar.
Claim 6
Dependent Claim
15 words
The method of claim 4, wherein the selective extraction step is conducted at 10°-30° C.
Claim 7
Dependent Claim
33 words
The method of claim 1, wherein said selective extraction is followed by a further selective extraction with an aqueous solution of urea containing mercaptoethanol in a concentration of 0.1 to 0.5 volume %.
Claim 8
Dependent Claim
20 words
The method of claim 1, wherein said selective extraction is preceded by extraction of soluble constituents into an ionic solvent.
Claim 9
Dependent Claim
24 words
The method of claim 1, wherein after completion of said selective extraction, at least one washing step which entrains denatured structural glycoproteins is performed.
Claim 10
Dependent Claim
27 words
The method of claim 1, wherein said washing step is carried out with distilled water at a temperature within the range of 30° C. to 60° C.
Claim 11
Dependent Claim
15 words
The method of claim 9, wherein the washing step is carried out at 45°-55° C.
Claim 12
Dependent Claim
14 words
The method of claim 1, wherein the selective extraction is conducted at 10°-30° C.
Claim 13
Dependent Claim
22 words
The method of claim 1, wherein, prior to said selective extraction, said starting non-demineralized bone tissue is subjected to a delipidation treatment.
Claim 14
Dependent Claim
33 words
The method of claim 12, wherein said selective extraction is followed by a further selective extraction with an aqueous solution of urea containing mercaptoethanol in a concentration of 0.1 to 0.5 volume %.
Claim 15
Dependent Claim
46 words
The method of claim 12, wherein said delipidation is carried out by contacting the starting non-demineralized bone tissue with a mixture of chloroform and methanol or a mixture of ethanol and dichloromethane in a proportion of approximately 10 liters to 1 kg of non-demineralized bone tissue.
Claim 16
Dependent Claim
35 words
The method of claim 14, wherein said selective extraction is followed by a further selective extraction with an aqueous solution of urea containing mercaptoethanol in a concentration of 0 1 to 0 5 volume %.
Claim 17
Dependent Claim
34 words
The method of claim 12, wherein said selective extraction is followed by a further selective extraction with an aqueous solution of urea containing mercaptoethanol in a concentration of 0.1 to 0. 5 volume %.
Claim 18
Dependent Claim
21 words
The method of claim 12, wherein said selective extraction is preceded by extraction of the soluble constituents into an ionic solvent.
Claim 19
Dependent Claim
31 words
An osteoplasty material obtained by a method in accordance with claim 1, wherein said osteoplasty material preserves the original and natural bone structure in which non-denatured type I collagen is integrated.
Claim 20
Dependent Claim
19 words
The osteoplasty material of claim 18, containing structural collagen in a proportion within the range of 20 to 40%.
Claim 21
Dependent Claim
20 words
The osteoplasty material of claim 19, wherein the structural collagen present is in a proportion within the range of 25-35%.
Claim 22
Dependent Claim
53 words
The osteoplasty material in accordance with claim 18, containing a proportion of lipids lower than 15%, a proportion of proteins within the range of 25 to 45%, a proportion of calcium of 10 to 30%, a proportion of phosphorus of 5 to 20%, and a water content lower than 10%, by dry analysis.
Claim 23
Dependent Claim
13 words
The osteoplasty material of claim 21, wherein the ratio calcium/phosphorus is from 1-2.2.
Claim 24
Dependent Claim
37 words
The osteoplasty material of claim 18, wherein said osteoplasty material is formed into parallelepipedal blocks, pyramidal frustums, lamellae, centro-medullary end-caps, disks or powder optionally amalgamated by means of a binder of biological origin or of synthetic origin.
Claim 25
Independent Claim
123 words
A method for producing an osteoplasty material, comprising (a) providing a starting non-demineralized bone tissue of human or animal origin containing antigenic, non-collagenous structural proteins and structural collagen, (b) delipidating the non-demineralized bone tissue of (a) to remove free lipids therefrom, (c) subjecting said bone tissue to at least one selective extraction of the antigenic proteins with an aqueous selective extraction agent containing urea at a sufficient concentration and a sufficient temperature to selectively decompose the antigenic non-collagenous protein structure without attacking type I collagen, (d) recovering an osteoplasty material for bone implants having the natural bone structure of the starting bone tissue and type I collagen integrated therein in non-denatured form, and (e) optionally washing and rinsing the osteoplasty material of (d).
Claim 26
Independent Claim
123 words
A method for producing an osteoplasty material, comprising (a) providing a starting non-demineralized bone tissue of human or animal origin containing antigenic, non-collagenous structural proteins and structural collagen, (b) delipidating the non-demineralized bone tissue of (a) to remove free lipids therefrom, (c) subjecting said bone tissue to at least one selective extraction of the antigenic proteins with an aqueous selective extraction agent containing urea at a sufficient concentration and a sufficient temperature to selectively decompose the antigenic non-collagenous protein structure without attacking type I collagen, (d) recovering an osteoplasty material for bone implants having the natural bone structure of the starting bone tissue and type I collagen integrated therein in non-denatured form, and (e) optionally washing and rinsing the osteoplasty material of (d).
References Cited (Backward Citations)
US3318774A
Cited
US4440750A
Cited
US4485097A
Cited
US4434094A
Cited
GB2175807A
Cited
US4678470A
Cited
US5290558A
Cited
US5439684A
Cited
US5484601A
Cited
US5336616A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-17 | 2000-06-17 | 2000-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-17 | 2004-06-17 | 2004-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-17 | 2008-06-17 | 2008-12-17 | $7700.00 | Window Closed / Lapsed |