US5583016A
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Mammalian telomerase
Assignee: Geron Corp
•
Filing: 1994-10-27
•
Granted: 1996-12-10
•
Expires: 2014-10-27
Abstract
Abstract Nucleic acids comprising the RNA component of a mammalian telomerase are useful as pharmaceutical, therapeutic, and diagnostic reagents.
Inventors
- 👤 Bryant Villeponteau
- 👤 Junli Feng
- 👤 Walter Funk
- 👤 William H. Andrews
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:02
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12Q; C12Q1/00; C12Q1/68; C12Q1/6806; C12Q1/6813; C12Q1/6841; C12Q1/6876; C12Q1/6883; C12Q1/6886; C12Y; C12Y207/00; C12Y207/07; C12Y207/07049; A; A01; A01K; A01K2217/00; A01K2217/05; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121; C12N2310/13; C12N2310/15; C12N2310/30; C12N2310/31; C12N2310/315; C12N2310/32; C12N2310/321
Claims Analysis
Total Claims: 56
Claim 1
Independent Claim
51 words
An isolated and purified recombinant nucleic acid fragment comprising an oligonucleotide having a contiguous sequence of at least 25 nucleotides in a sequence complementary or identical to a human genomic DNA sequence encoding the RNA component of human telomerase located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 2
Dependent Claim
51 words
An isolated and purified recombinant nucleic acid fragment comprising an oligonucleotide having a contiguous sequence of at least 25 nucleotides in a sequence complementary or identical to a human genomic DNA sequence encoding the RNA component of human telomerase located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 3
Dependent Claim
14 words
The isolated and purified recombinant nucleic acid of claim 1 that is an oligodeoxyribonucleotide.
Claim 4
Dependent Claim
14 words
The isolated and purified recombinant nucleic acid of claim 1 that is an oligoribonucleotide.
Claim 5
Dependent Claim
23 words
The isolated and purified recombinant nucleic acid of claim 1 that is complementary to the DNA encoding the RNA component of human telomerase.
Claim 6
Dependent Claim
16 words
The isolated and purified recombinant nucleic acid of claim 1 that is plasmid pGRN33 (ATCC 75926).
Claim 7
Dependent Claim
17 words
The isolated and purified recombinant nucleic acid of claim 1 that is lambda clone 28-1 (ATCC 75925).
Claim 8
Dependent Claim
24 words
The isolated and purified recombinant nucleic acid of claim 1 comprising the sequence: ##STR8## (SEQ ID NO: 3), wherein "T" also can be "U".
Claim 9
Dependent Claim
24 words
The isolated and purified recombinant nucleic acid of claim 1 comprising the sequence: ##STR9## (SEQ ID NO: 3) wherein "T" also can be "U".
Claim 10
Dependent Claim
18 words
The isolated and purified recombinant nucleic acid of claim 3 comprising the sequence: ##STR10## (SEQ ID NO: 1).
Claim 11
Dependent Claim
27 words
The isolated and purified recombinant nucleic acid of claim 1 further comprising a promoter positioned to drive transcription of an RNA complementary in sequence to said oligonucleotide.
Claim 12
Dependent Claim
21 words
The isolated and purified recombinant nucleic acid of claim 10 wherein the contiguous sequence is at least 50 nucleotides in length.
Claim 13
Dependent Claim
21 words
The isolated and purified recombinant nucleic acid of claim 10 wherein the contiguous sequence is at least 200 nucleotides in length.
Claim 14
Dependent Claim
21 words
The isolated and purified recombinant nucleic acid of claim 10 wherein the contiguous sequence is at least 400 nucleotides in length.
Claim 15
Dependent Claim
21 words
The isolated and purified recombinant nucleic acid of claim 10 wherein the contiguous sequence is at most 50 nucleotides in length.
Claim 16
Dependent Claim
25 words
The isolated and purified recombinant nucleic acid of claim 10 wherein said recombinant nucleic acid functions to produce the oligonucleotide in a prokaryotic host cell.
Claim 17
Dependent Claim
25 words
The isolated and purified recombinant nucleic acid of claim 10 wherein said recombinant nucleic acid functions to produce the oligonucleotide in a eukaryotic host cell.
Claim 18
Dependent Claim
41 words
The isolated and purified recombinant nucleic acid of claim 16 wherein said recombinant nucleic acid functions to produce the oligonucleotide in a human cell such that the RNA is capable of being assembled by the cell into a functional telomerase molecule.
Claim 19
Dependent Claim
24 words
The isolated and purified recombinant nucleic acid of claim 10 wherein the oligonucleotide comprises a human gene for the RNA component of human telomerase.
Claim 20
Dependent Claim
24 words
The isolated and purified recombinant nucleic acid of claim 10 comprising the sequence: ##STR11## (SEQ ID NO: 3), wherein "T" also can be "U".
Claim 21
Dependent Claim
24 words
The isolated and purified recombinant nucleic acid of claim 10 comprising the sequence: ##STR12## (SEQ ID NO: 3) wherein "T" also can be "U".
Claim 22
Dependent Claim
28 words
The isolated and purified recombinant nucleic acid of claim 1 comprising a promoter positioned to specifically drive the transcription of an RNA complementary in sequence to said oligonucleotide.
Claim 23
Dependent Claim
46 words
An isolated oligonucleotide between 25 and 1000 nucleotides in length in a sequence identical or complementary to a contiguous sequence contained within a human genomic DNA sequence "encoding the RNA component of human telomerase" located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 24
Dependent Claim
46 words
An isolated oligonucleotide between 25 and 1000 nucleotides in length in a sequence identical or complementary to a contiguous sequence contained within a human genomic DNA sequence "encoding the RNA component of human telomerase" located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 25
Dependent Claim
12 words
The isolated oligonucleotide of claim 22 at most 200 nucleotides in length.
Claim 26
Dependent Claim
12 words
The isolated oligonucleotide of claim 22 at most 50 nucleotides in length.
Claim 27
Dependent Claim
12 words
The isolated oligonucleotide of claim 22 at least 50 nucleotides in length.
Claim 28
Dependent Claim
12 words
The isolated oligonucleotide of claim 25 at most 200 nucleotides in length.
Claim 29
Dependent Claim
12 words
The isolated oligonucleotide of claim 22 at least 200 nucleotides in length.
Claim 30
Dependent Claim
12 words
The isolated oligonucleotide of claim 22 at least 400 nucleotides in length.
Claim 31
Dependent Claim
12 words
The isolated oligonucleotide of any of claims 22-28 further comprising a label.
Claim 32
Dependent Claim
19 words
The isolated oligonucleotide of claim 29 wherein the label is a radioactive label, a fluorescent label, biotin or avidin.
Claim 33
Dependent Claim
6 words
Isolated RNA component of human telomerase.
Claim 34
Dependent Claim
6 words
Isolated RNA component of human telomerase.
Claim 35
Dependent Claim
11 words
An oligonucleotide of SEQ ID NO: 22, 23, 24 or 25.
Claim 36
Dependent Claim
11 words
An oligonucleotide of SEQ ID NO: 22, 23, 24 or 25.
Claim 37
Dependent Claim
53 words
A host cell transformed with a recombinant nucleic acid comprising an oligonucleotide having a contiguous sequence of at least 25 nucleotides in a sequence complementary or identical to a human genomic DNA sequence encoding the RNA component of human telomerase located in an ˜2.5 kb HindIII-Sac I insert of plasmid pGRN33 (ATCC 75926).
Claim 38
Dependent Claim
53 words
A host cell transformed with a recombinant nucleic acid comprising an oligonucleotide having a contiguous sequence of at least 25 nucleotides in a sequence complementary or identical to a human genomic DNA sequence encoding the RNA component of human telomerase located in an ˜2.5 kb HindIII-Sac I insert of plasmid pGRN33 (ATCC 75926).
Claim 39
Dependent Claim
11 words
The host cell of claim 33 transformed with pGRN33 (ATCC 75926).
Claim 40
Dependent Claim
13 words
The host cell of claim 33 transformed with lambda clone 28-1 (ATCC 75925).
Claim 41
Dependent Claim
30 words
The host cell of claim 33 wherein the recombinant nucleic acid further comprises a promoter positioned to drive the transcription of an RNA having a sequence complementary to the oligonucleotide.
Claim 42
Dependent Claim
11 words
The host cell of claim 33 that is a eukaryotic cell.
Claim 43
Dependent Claim
39 words
The host cell of claim 37 wherein the recombinant nucleic acid encodes an RNA molecule that can associate with protein components of human telomerase to produce telomerase activity capable of adding sequences of repeating units of nucleotides to telomeres.
Claim 44
Dependent Claim
12 words
The host cell of claim 38, wherein said repeating unit is 5'-TTAGGG-3'.
Claim 45
Dependent Claim
13 words
The host cell of claim 38, wherein said repeating unit is not 5'-TTAGGG-3'.
Claim 46
Dependent Claim
18 words
The host cell of claim 37, wherein said RNA molecule comprises the sequence: ##STR13## (SEQ ID NO: 1).
Claim 47
Dependent Claim
24 words
The host cell of claim 37, wherein the recombinant nucleic acid comprises the sequence: ##STR14## (SEQ ID NO:3), wherein "T" also can be "U".
Claim 48
Dependent Claim
25 words
The host cell of claim 37, wherein the recombinant nucleic acid comprises the sequence: ##STR15## (SEQ ID NO: 3) wherein "T" also can be "U".
Claim 49
Independent Claim
43 words
A method for producing the RNA component of human telomerase comprising the step of culturing a eukaryotic host cell transformed with a recombinant nucleic acid comprising a promoter positioned to drive the transcription of an oligonucleotide encoding an RNA component of human telomerase.
Claim 50
Independent Claim
43 words
A method for producing the RNA component of human telomerase comprising the step of culturing a eukaryotic host cell transformed with a recombinant nucleic acid comprising a promoter positioned to drive the transcription of an oligonucleotide encoding an RNA component of human telomerase.
Claim 51
Dependent Claim
36 words
The method of claim 44 wherein the oligonucleotide encoding the RNA component of human telomerase includes a sequence from a human genomic DNA sequence located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 52
Dependent Claim
12 words
The method of claim 44 wherein the cell is a human cell.
Claim 53
Independent Claim
97 words
A method for producing a recombinant telomerase enzyme, said method comprising transforming a eukaryotic host cell capable of expressing protein components of telomerase, with a recombinant nucleic acid comprising a promoter positioned to drive the transcription of an oligonucleotide encoding the RNA component of human telomerase, said recombinant nucleic acid functioning to produce the oligonucleotide in a eukaryotic cell, and culturing said host cells transformed with said vector under conditions such that the protein components and RNA component are expressed and assemble to form an active telomerase molecule capable of adding sequences to telomeres of chromosomal DNA.
Claim 54
Independent Claim
97 words
A method for producing a recombinant telomerase enzyme, said method comprising transforming a eukaryotic host cell capable of expressing protein components of telomerase, with a recombinant nucleic acid comprising a promoter positioned to drive the transcription of an oligonucleotide encoding the RNA component of human telomerase, said recombinant nucleic acid functioning to produce the oligonucleotide in a eukaryotic cell, and culturing said host cells transformed with said vector under conditions such that the protein components and RNA component are expressed and assemble to form an active telomerase molecule capable of adding sequences to telomeres of chromosomal DNA.
Claim 55
Dependent Claim
41 words
The method of claim 47 wherein the RNA has a sequence identical to a contiguous sequence encoding the RNA component of human telomerase from a human genomic DNA sequence located in an ˜2.5 kb HindIII-SacI insert of plasmid pGRN33 (ATCC 75926).
Claim 56
Dependent Claim
12 words
The method of claim 48 wherein the cell is a human cell.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-10 | 2000-06-10 | 2000-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-10 | 2004-06-10 | 2004-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-10 | 2008-06-10 | 2008-12-10 | $7700.00 | Window Closed / Lapsed |