US5270201A
Expired / Lapsed
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Artificial chromosome vector
Assignee: General Hospital Corp
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Filing: 1992-03-30
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Granted: 1993-12-14
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Expires: 2012-03-30
Abstract
Abstract The present invention relates to a recombinant DNA molecule which contains the telomere and, optionally, the centromere of a higher eukaryote, particularly a plant, the telomere itself, the centromere itself, a method of producing a polypeptide in a recipient cell which utilizes said recombinant DNA molecule, host cells transformed with said recombinant molecule, and uses for said recombinant molecule.
Inventors
- 👤 Eric J. Richards
- 👤 Frederick M. Ausubel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 22:05
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/67; C12N15/68
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
67 words
A recombinant DNA construct comprising a telomere, said telomere consisting essentially of tandem repeats of the sequence 5'-CCCTAAA-3' in sufficient quantity to provide a telomere property to a linear double-stranded DNA construct when said telomere is double-stranded and is oriented such that the C-rich 5' end of each tandem repeat is closer to the blunt end of the telomere than the A-rich 3' end of each repeat.
Claim 2
Dependent Claim
67 words
A recombinant DNA construct comprising a telomere, said telomere consisting essentially of tandem repeats of the sequence 5'-CCCTAAA-3' in sufficient quantity to provide a telomere property to a linear double-stranded DNA construct when said telomere is double-stranded and is oriented such that the C-rich 5' end of each tandem repeat is closer to the blunt end of the telomere than the A-rich 3' end of each repeat.
Claim 3
Dependent Claim
13 words
The recombinant DNA construct of claim 1, which additionally comprises a yeast centromere.
Claim 4
Dependent Claim
14 words
The recombinant construct of claim 1, which additionally comprises a yeast autonomous replicating sequence.
Claim 5
Dependent Claim
13 words
The recombinant construct of claim 1, which additionally comprises a selectable marker gene.
Claim 6
Dependent Claim
81 words
A recombinant DNA construct comprising a telomere of a higher eukaryotic organism, a yeast centromere, and a yeast autonomous replicating sequence, said telomere consisting essentially of tandem repeats of the sequence 5'-CCCTAAA-3' in sufficient quantity to provide a telomere property to a linear double-stranded DNA construct when said telomere is double-stranded and is oriented such that the C-rich 5' end of each tandem repeat is closer to the blunt end of the telomere than the A-rich 3' end of each repeat.
Claim 7
Dependent Claim
81 words
A recombinant DNA construct comprising a telomere of a higher eukaryotic organism, a yeast centromere, and a yeast autonomous replicating sequence, said telomere consisting essentially of tandem repeats of the sequence 5'-CCCTAAA-3' in sufficient quantity to provide a telomere property to a linear double-stranded DNA construct when said telomere is double-stranded and is oriented such that the C-rich 5' end of each tandem repeat is closer to the blunt end of the telomere than the A-rich 3' end of each repeat.
Claim 8
Dependent Claim
14 words
The recombinant DNA construct of claim 5, which additionally comprises a selectable marker gene.
Claim 9
Dependent Claim
21 words
The recombinant DNA construct of any one of claims 1 or 4, which is capable of being maintained as a chromosome.
Claim 10
Dependent Claim
10 words
A plasmid comprising the recombinant DNA construct of claim 7.
Claim 11
Dependent Claim
22 words
The plasmid of claim 8, wherein said plasmid further comprises an origin of replication and a selection marker that function in bacteria.
Claim 12
Dependent Claim
11 words
The plasmid of claim 9, wherein said bacteria is E. coli.
Claim 13
Dependent Claim
22 words
The plasmid of claim 8, wherein said plasmid further comprises an origin of replication and a selection marker that function in yeast.
Claim 14
Dependent Claim
11 words
The plasmid of claim 11, wherein said yeast is S. cerevisiae.
Claim 15
Dependent Claim
12 words
The construct of claim 7, which additionally comprises a desired gene sequence.
Claim 16
Dependent Claim
80 words
The construct of claim 13, wherein said desired gene sequence is selected from the group consisting of: (1) a gene sequence of a hormone gene; (2) a gene sequence of an antibiotic resistance gene; (3) a gene sequence of a nitrogen fixation gene; (4) a gene sequence of a plant pathogen defense gene; (5) a gene sequence of a plant stress-induced gene; (6) a gene sequence of a toxin gene; and (7) a gene sequence of a seed storage gene.
Claim 17
Dependent Claim
16 words
The construct of claim 14, wherein said construct is capable of expressing said desired gene sequence.
Claim 18
Dependent Claim
18 words
The construct of claim 14, wherein said construct is capable of expressing said desired gene in a prokaryote.
Claim 19
Dependent Claim
18 words
The construct of claim 14, wherein said construct is capable of expressing said desired gene in a eukaryote.
Claim 20
Dependent Claim
12 words
The construct of claim 17, wherein said eukaryote is a higher eukaryote.
Claim 21
Dependent Claim
12 words
The construct of claim 18, wherein said higher eukaryote is a plant.
Claim 22
Dependent Claim
12 words
A recombinant DNA construct comprising the pAtT4 plasmid, Accession Number ATCC 67577.
Claim 23
Dependent Claim
12 words
A recombinant DNA construct comprising the pAtT4 plasmid, Accession Number ATCC 67577.
Claim 24
Dependent Claim
10 words
A recombinant DNA construct comprising the sequence of FIG. 4.
Claim 25
Dependent Claim
10 words
A recombinant DNA construct comprising the sequence of FIG. 4.
Claim 26
Dependent Claim
17 words
A host cell transformed with the recombinant DNA construct of any one of claims 1 or 5.
Claim 27
Dependent Claim
11 words
The host cell of claim 22, which is a eukaryotic cell.
Claim 28
Dependent Claim
12 words
The host cell of claim 23, which is a higher eukaryotic cell.
Claim 29
Dependent Claim
11 words
The host cell of claim 24, which is a plant cell.
References Cited (Backward Citations)
EP0048081A2
Cited
WO1986000089A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1996-12-13 | 1997-06-14 | 1997-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-13 | 2001-06-14 | 2001-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-13 | 2005-06-14 | 2005-12-14 | $7700.00 | Window Closed / Lapsed |