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US5580703A
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Human adeno-associated virus integration site DNA and uses thereof
Assignee: Wyeth Holdings LLC
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Filing: 1994-09-20
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Granted: 1996-12-03
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Expires: 2014-09-20
Abstract
Abstract The present invention relates to an isolated nucleic acid fragment comprising a nucleic acid sequence corresponding to a human AAV integration site, or a subsequence thereof which is site specific to the AAV integration site.
Inventors
- 👤 Robert M. Kotin
- 👤 Kenneth I. Berns
- 👤 Ralph M. Linden
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:23
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K48/00; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14111; C12N2750/14122; C12N2750/14141; C12N2750/14143
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
13 words
An essentially pure nucleic acid having the sequence in SEQ ID NO: 1.
Claim 2
Dependent Claim
13 words
An essentially pure nucleic acid having the sequence in SEQ ID NO: 1.
Claim 3
Dependent Claim
11 words
The nucleic acid of claim 1 which is about 4 kb.
Claim 4
Dependent Claim
13 words
An essentially pure nucleic acid having nucleotides 1609-2308 in SEQ ID NO: 1.
Claim 5
Dependent Claim
13 words
An essentially pure nucleic acid having nucleotides 1609-2308 in SEQ ID NO: 1.
Claim 6
Dependent Claim
171 words
An essentially pure nucleic acid having the following characteristics: (a) a TATA-less promoter located at nucleotide positions 1-900 in SEQ ID NO: 1 comprising three SP1-like binding sites, a cyclic AMP responsive element and at least six upstream binding factor 1 sequences, wherein: (i) the SP1-like binding sites are located at nucleotides 292-299, 358-373 and 416-423 in SEQ ID NO: 1; (ii) the cyclic AMP responsive element has the nucleotide sequence TGACGTCA and is located at nucleotides 278-285 in SEQ ID NO: 1; (iii) the six upstream binding factor 1 sequences have the sequence CGGCC or the reverse complement GGCCG and are located at nucleotide positions 88, 346, 446, 596, 682 and 683 in SEQ ID NO: 1; (b) a gene that is transcriptionally active located at nucleotides 1609-2308 in SEQ ID NO: 1; (c) three recombination event sites located at nucleotides 1013-1016, 1128-1134 and 3346-3349; (d) at least three additional upstream binding factor 1 sequences located at nucleotide positions 2117, 2770 and 3429; and (e) is isolatable from chromosome 19.
Claim 7
Dependent Claim
171 words
An essentially pure nucleic acid having the following characteristics: (a) a TATA-less promoter located at nucleotide positions 1-900 in SEQ ID NO: 1 comprising three SP1-like binding sites, a cyclic AMP responsive element and at least six upstream binding factor 1 sequences, wherein: (i) the SP1-like binding sites are located at nucleotides 292-299, 358-373 and 416-423 in SEQ ID NO: 1; (ii) the cyclic AMP responsive element has the nucleotide sequence TGACGTCA and is located at nucleotides 278-285 in SEQ ID NO: 1; (iii) the six upstream binding factor 1 sequences have the sequence CGGCC or the reverse complement GGCCG and are located at nucleotide positions 88, 346, 446, 596, 682 and 683 in SEQ ID NO: 1; (b) a gene that is transcriptionally active located at nucleotides 1609-2308 in SEQ ID NO: 1; (c) three recombination event sites located at nucleotides 1013-1016, 1128-1134 and 3346-3349; (d) at least three additional upstream binding factor 1 sequences located at nucleotide positions 2117, 2770 and 3429; and (e) is isolatable from chromosome 19.
Claim 8
Dependent Claim
10 words
A recombinant vector comprising the nucleic acid of claim 1.
Claim 9
Dependent Claim
10 words
A recombinant vector comprising the nucleic acid of claim 4.
Claim 10
Dependent Claim
24 words
A primer for use in detection of adeno-associated virus integration comprising an essentially pure nucleic acid having the sequence in SEQ ID NO: 1.
Claim 11
Dependent Claim
24 words
A primer for use in detection of adeno-associated virus integration comprising an essentially pure nucleic acid having the sequence in SEQ ID NO: 1.
Claim 12
Dependent Claim
49 words
A primer selected from the group consisting of: SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22 and SEQ ID NO: 23.
Claim 13
Dependent Claim
49 words
A primer selected from the group consisting of: SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22 and SEQ ID NO: 23.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-03 | 2000-06-03 | 2000-12-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-03 | 2004-06-03 | 2004-12-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-03 | 2008-06-03 | 2008-12-03 | $7700.00 | Window Closed / Lapsed |