Patent US2643831A successfully refreshed from Google Patents.
Patent US5567413A successfully refreshed from Google Patents.
US5567413A
Expired / Lapsed
US
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Flexible amphiphilic microbubbles for ultrasound
Assignee: GE Healthcare AS
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Filing: 1995-06-06
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Granted: 1996-10-22
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Expires: 2015-06-06
Abstract
Abstract The invention relates to diagnostic ultrasound contrast agents comprising vesicles comprising an amphiphilic phospholipid material capable of formation of gas-containing vesicles wherein the vesicles contain gas which comprises a low molecular weight fluorinated hydrocarbon.
Inventors
- 👤 Jo Klaveness
- 👤 Hanno Priebe
- 👤 Pål Rongved
- 👤 Lars Stubberud
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:03
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/22; A61K49/222; A61K49/223
Claims Analysis
Total Claims: 50
Claim 1
Independent Claim
30 words
A diagnostic ultrasound contrast agent comprising stabilized microbubbles of a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon encapsulated by flexible material comprising non-proteinaceous crosslinked or polymerized amphiphilic moieties.
Claim 2
Dependent Claim
30 words
A diagnostic ultrasound contrast agent comprising stabilized microbubbles of a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon encapsulated by flexible material comprising non-proteinaceous crosslinked or polymerized amphiphilic moieties.
Claim 3
Dependent Claim
24 words
A method of enhancing ultrasound images of a vascular system comprising administering to said system a diagnostic ultrasound contrast agent according to claim 1.
Claim 4
Dependent Claim
24 words
Vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 5
Dependent Claim
24 words
Vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 6
Dependent Claim
11 words
Vesicles as claimed in claim 3 wherein said hydrocarbon is perfluorinated.
Claim 7
Dependent Claim
26 words
Vesicles as claimed in claim 3 wherein said phospholipid material comprises hydrophilic groups selected from the group consisting of choline, ethanolamine, serine, glycerol, pentoses and hexoses.
Claim 8
Dependent Claim
16 words
Vesicles as claimed in claim 5 wherein said phospholipid material comprises a lecithin or derivative thereof.
Claim 9
Dependent Claim
10 words
An aqueous dispersion comprising vesicles as claimed in claim 3.
Claim 10
Dependent Claim
10 words
An aqueous dispersion comprising vesicles as claimed in claim 4.
Claim 11
Dependent Claim
10 words
An aqueous dispersion comprising vesicles as claimed in claim 5.
Claim 12
Dependent Claim
10 words
An aqueous dispersion comprising vesicles as claimed in claim 6.
Claim 13
Dependent Claim
14 words
Vesicles as claimed in claim 3 wherein said phospholipid material is crosslinked or polymerized.
Claim 14
Dependent Claim
35 words
Vesicles as claimed in claim 11 wherein said crosslinked or polymerized phospholipid material contains biodegradable linkages selected from the group consisting of amide, imide, imine, ester, anhydride, acetal, carbamate, carbonate, carbonate ester and disulphide groups.
Claim 15
Dependent Claim
17 words
Vesicles as claimed in claim 12 wherein said crosslinked or polymerized phospholipid material contains biodegradable amide linkages.
Claim 16
Dependent Claim
14 words
Vesicles as claimed in claim 12 wherein said phospholipid material contains biodegradable crosslinking groups.
Claim 17
Dependent Claim
89 words
Vesicles as claimed in claim 14 wherein said biodegradable crosslinking groups include units of formula --(Y).sub.n.CO.O.C(R.sup.1 R.sup.2).O.CO.(Z).sub.n -- Y and Z, which may be the same or different, are --O--, --S-- or --NR 3 --; R 1 and R 2 , which may be the same or different, are hydrogen atoms or carbon-attached monovalent organic groups or together represent a carbon-attached divalent organic group; R 3 is a hydrogen atom or an organic group; and the symbols n, which may be the same or different, are zero or 1.
Claim 18
Dependent Claim
15 words
Vesicles as claimed in claim 11 obtained from polymerizable phospholipid material containing unsaturated lipophilic chains.
Claim 19
Dependent Claim
25 words
Vesicles as claimed in claim 16 wherein said unsaturated lipophilic chains are oleyl or linoleyl groups or contain diacetylene groupings or acryloyl or methacryloyl groupings.
Claim 20
Dependent Claim
17 words
Vesicles as claimed in claim 11 which are crosslinked between the hydrophilic parts of the phospholipid moieties.
Claim 21
Dependent Claim
21 words
Vesicles as claimed in claim 3 which comprise a fluorinated low molecular weight hydrocarbon stabilized by monolayers of said phospholipid material.
Claim 22
Dependent Claim
11 words
Vesicles as claimed in claim 19 wherein said hydrocarbon is perfluorinated.
Claim 23
Dependent Claim
24 words
Vesicles as claimed in claim 11 which comprise a fluorinated low molecular weight hydrocarbon stabilized by monolayers of said crosslinked or polymerized phospholipid material.
Claim 24
Dependent Claim
11 words
Vesicles as claimed in claim 21 wherein said hydrocarbon is perfluorinated.
Claim 25
Dependent Claim
14 words
Vesicles as claimed in claim 3 having an average size of 0.114 10 μm.
Claim 26
Dependent Claim
13 words
Vesicles as claimed in claim 23 having an average size of 1-7 μm.
Claim 27
Dependent Claim
13 words
Vesicles as claimed in claim 11 having an average size of 0.1-10 μm.
Claim 28
Dependent Claim
13 words
Vesicles as claimed in claim 25 having an average size of 1-7 μm.
Claim 29
Dependent Claim
20 words
Vesicles as claimed in claim 11 wherein the crosslinked or polymerized phospholipid material comprises phospholipid material reacted with preformed polymer.
Claim 30
Dependent Claim
10 words
An aqueous dispersion comprising vesicles as claimed in claim 11.
Claim 31
Dependent Claim
12 words
A diagnostic ultrasound contrast agent comprising vesicles as claimed in claim 11.
Claim 32
Dependent Claim
18 words
A contrast agent as claimed in claim 29 having a half-life in vivo of 1 to 48 hours.
Claim 33
Dependent Claim
18 words
A contrast agent as claimed in claim 30 having a half-life in vivo of 1 to 12 hours.
Claim 34
Dependent Claim
17 words
A contrast agent as claimed in claim 29 wherein said vesicles are dispersed in an aqueous carrier.
Claim 35
Dependent Claim
37 words
A process for the preparation of a contrast agent which comprises generating vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 36
Dependent Claim
37 words
A process for the preparation of a contrast agent which comprises generating vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 37
Dependent Claim
36 words
A process as claimed in claim 33 which comprises shaking or sonicating an amphiphile-containing mixture comprising a phospholipid in the presence of a fluorinated low molecular weight hydrocarbon to generate a fluid dispersion of said vesicles.
Claim 38
Dependent Claim
24 words
A process as claimed in claim 34 wherein an aqueous amphiphile-containing mixture comprising a phospholipid is used to generate an aqueous dispersion of vesicles.
Claim 39
Dependent Claim
16 words
A process as claimed in claim 34 wherein the contrast agent is isolated by freeze drying.
Claim 40
Dependent Claim
12 words
A process as claimed in claim 33 wherein said hydrocarbon is perfluorinated.
Claim 41
Dependent Claim
39 words
A process for the preparation of a contrast agent which comprises crosslinking or polymerizing vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 42
Dependent Claim
39 words
A process for the preparation of a contrast agent which comprises crosslinking or polymerizing vesicles comprising flexible amphiphilic phospholipid material capable of formation of gas-containing vesicles, said vesicles containing a biocompatible gas comprising a fluorinated low molecular weight hydrocarbon.
Claim 43
Dependent Claim
21 words
A process as claimed in claim 38 wherein crosslinking or polymerization is effected between the hydrophilic parts of the phospholipid material.
Claim 44
Dependent Claim
20 words
A process as claimed in claim 38 wherein crosslinking or polymerization is effected by self condensation of the phospholipid material.
Claim 45
Dependent Claim
17 words
A process as claimed in claim 38 wherein the phospholipid material is reacted with a preformed polymer.
Claim 46
Dependent Claim
16 words
A process as claimed in claim 38 wherein the contrast agent is isolated by freeze drying.
Claim 47
Dependent Claim
12 words
A process as claimed in claim 38 wherein said hydrocarbon is perfluorinated.
Claim 48
Dependent Claim
10 words
A contrast agent prepared by the process of claim 33.
Claim 49
Dependent Claim
24 words
A method of enhancing ultrasound images of a vascular system comprising administering to said system a diagnostic ultrasound contrast agent according to claim 29.
Claim 50
Dependent Claim
24 words
A method of enhancing ultrasound images of a vascular system comprising administering to said system a diagnostic ultrasound contrast agent according to claim 44.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-10-22 | 2000-04-22 | 2000-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-10-22 | 2004-04-22 | 2004-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-10-22 | 2008-04-22 | 2008-10-22 | $7700.00 | Window Closed / Lapsed |