US5556533A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Voltage applying method for hydrogen-type enzyme electrode
Assignee: A&D Holon Holdings Co Ltd
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Filing: 1995-06-06
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Granted: 1996-09-17
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Expires: 2015-06-06
Abstract
Abstract A voltage applying method for a hydrogen type enzyme electrode in a chemical sensor and using a pair of working electrodes, a reference electrode and a counter electrode includes detecting a contact of a test specimen with the enzyme electrode; keeping a potential applied to the working electrodes at a first potential of substantially zero for a first preset time; applying a second potential that is higher than a hydrogen peroxide detect potential to the working electrodes for a second preset time; dropping the second potential to a third potential below zero potential; and sweeping from the first potential to a fourth potential higher than said hydrogen peroxide detect potential at a fixed rate. Other variations of the method are disclosed.
Inventors
- 👤 Yoshiteru Nozoe
- 👤 Kazuharu Murata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 17:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/4875; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
107 words
A voltage applying method for a hydrogen enzyme electrode having a pair of working electrodes, a reference electrode and a counter electrode comprising: detecting a contact of a test specimen with said enzyme electrode; keeping a potential applied to said working electrodes at a first potential of substantially zero for a first preset time; applying a second potential which is higher than a hydrogen peroxide detect potential to said working electrodes for a second preset time; dropping said second potential to a third potential below zero potential; sweeping from said third potential to a fourth potential higher than said hydrogen peroxide detect potential at a fixed rate.
Claim 2
Dependent Claim
107 words
A voltage applying method for a hydrogen enzyme electrode having a pair of working electrodes, a reference electrode and a counter electrode comprising: detecting a contact of a test specimen with said enzyme electrode; keeping a potential applied to said working electrodes at a first potential of substantially zero for a first preset time; applying a second potential which is higher than a hydrogen peroxide detect potential to said working electrodes for a second preset time; dropping said second potential to a third potential below zero potential; sweeping from said third potential to a fourth potential higher than said hydrogen peroxide detect potential at a fixed rate.
Claim 3
Dependent Claim
48 words
A voltage applying method according to claim 1, wherein said detecting step comprising: applying a preset potential to said pair of working electrodes; detecting a preset current between said pair of working electrodes and said counter electrode to confirm as to whether enzyme electrode contacts said test specimen.
Claim 4
Dependent Claim
19 words
A voltage applying method according to claim 1, wherein said first preset time is substantially 15 to 40 seconds.
Claim 5
Dependent Claim
18 words
A voltage applying method according to claim 1, wherein said hydrogen peroxide detect potential is substantially 600 mV.
Claim 6
Dependent Claim
18 words
A voltage applying method according to claim 4, wherein said third potential is substantially -400 to -200 mV.
Claim 7
Dependent Claim
16 words
A voltage applying method according to claim 1, wherein said fixed rate is substantially 100 mV/sec.
Claim 8
Dependent Claim
88 words
A voltage applying method according to claim 1, further comprising said value: simultaneously with said sweeping step, detecting one of a peak value of current in the range of the voltage sweep to said fourth potential, a current value at a potential separated from a potential causing the peak current value by a preset potential value, and a current value at a specific potential; and converting said detected current value into a concentration of a substance in said test specimen by comparing with a calibration curve previously charted.
Claim 9
Dependent Claim
104 words
A voltage applying method for a hydrogen enzyme electrode having a pair of working electrodes, a reference electrode and a counter electrode comprising: detecting a contact of a test specimen with said enzyme electrode; keeping a potential applied to said working electrodes at a first potential of substantially zero for a first preset time; flowing a first current which is larger than a hydrogen peroxide detect current to said working electrodes for a second preset time; dropping to a second potential below zero potential; sweeping from said second potential to a third potential higher than said hydrogen peroxide detect potential at a fixed rate.
Claim 10
Dependent Claim
104 words
A voltage applying method for a hydrogen enzyme electrode having a pair of working electrodes, a reference electrode and a counter electrode comprising: detecting a contact of a test specimen with said enzyme electrode; keeping a potential applied to said working electrodes at a first potential of substantially zero for a first preset time; flowing a first current which is larger than a hydrogen peroxide detect current to said working electrodes for a second preset time; dropping to a second potential below zero potential; sweeping from said second potential to a third potential higher than said hydrogen peroxide detect potential at a fixed rate.
Claim 11
Dependent Claim
46 words
A voltage applying method according to claim 8, wherein said detecting step comprising: applying a preset potential to said pair of working electrodes; detecting a preset current between said pair of working electrodes and said counter electrode to confirm enzyme electrode contact with said test specimen.
Claim 12
Dependent Claim
88 words
A voltage applying method according to claim 8, further comprising: simultaneously with said sweeping step, detecting one of a peak value of current in the range of the voltage sweep to said fourth potential, a current value at a potential separated from a potential causing the peak current value by a preset potential value, and a current value at a specific potential; and converting said detected current value into a concentration of a substance in said test specimen by comparing said value with a calibration curve previously charted.
Claim 13
Dependent Claim
35 words
A voltage applying method according to claim 8, further comprising, before dropping to a second potential step, keeping a final potential that is reached when said preset time is passed at a third preset time.
Claim 14
Dependent Claim
88 words
A voltage applying method according to claim 11, further comprising: simultaneously with said sweeping step, detecting one of a peak value of current in the range of the voltage sweep to said fourth potential, a current value at a potential separated from a potential causing the peak current value by a preset potential value, and a current value at a specific potential; and converting said detected current value into a concentration of a substance in said test specimen by comparing said value with a calibration curve previously charted.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-09-17 | 2000-03-18 | 2000-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-09-17 | 2004-03-18 | 2004-09-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-09-17 | 2008-03-18 | 2008-09-17 | $7700.00 | Window Closed / Lapsed |