US4528270A
Expired / Lapsed
US
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Electrochemical method for detection and classification of microbial cell
Assignee: Advance Kaihatsu Kenkyujo KK
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Filing: 1983-10-27
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Granted: 1985-07-09
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Expires: 2003-10-27
Abstract
Abstract An electrochemical method for detecting and classifying microbial cells by applying a sweep potential between a working electrode and a counter electrode while microbial cells are brought into contact with the working electrode and, then, measuring the generated current between the electrodes. Cell numbers and types of cells of microorganisms, animals, and plants are determined from the peak current of cyclic voltammetry.
Inventors
- 👤 Tadashi Matsunaga
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 15:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/48707; G01N33/48728
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
74 words
An electrochemical method for detecting and classifying living microbial cells comprising the steps of: bringing the living microbial cells into contact with a working electrode; applying a sweep potential between the working electrode as an anode and a counter electrode in the presence of 4,4'bipyridine as a current increasing agent; and then measuring the generated anodic current between the electrodes to detect an anodic peak current that identifies the presence of living microbial cells.
Claim 2
Dependent Claim
74 words
An electrochemical method for detecting and classifying living microbial cells comprising the steps of: bringing the living microbial cells into contact with a working electrode; applying a sweep potential between the working electrode as an anode and a counter electrode in the presence of 4,4'bipyridine as a current increasing agent; and then measuring the generated anodic current between the electrodes to detect an anodic peak current that identifies the presence of living microbial cells.
Claim 3
Dependent Claim
36 words
The method of claim 1, wherein said sweep potential and a minute potential which is overlapped on said sweep potential to generate a differential current, are simultaneously applied between the working electrode and the counter electrode.
Claim 4
Dependent Claim
20 words
The method of claim 1, wherein the working and counter electrodes are inserted into a suspension of the microbial cells.
Claim 5
Dependent Claim
23 words
The method of claim 1, wherein the cell numbers are determined by measuring the value of the peak current of the generated current.
Claim 6
Dependent Claim
26 words
The method of claim 1, wherein the types of microbial cells are identified by determining the correlation curve between the generated current and the sweep potential.
Claim 7
Dependent Claim
20 words
The method of claim 1, wherein the generated current is measured by applying a cyclic sweep potential between the electrodes.
References Cited (Backward Citations)
US3827948A
Cited
US4055799A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-08 | 1989-01-07 | 1989-07-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-08 | 1993-01-07 | 1993-07-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-08 | 1997-01-07 | 1997-07-09 | $7700.00 | Window Closed / Lapsed |