Patent US5552274A successfully refreshed from Google Patents.
US5552274A
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Method for detecting target sequences by oscillation frequency
Assignee: Terumo Corp
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Filing: 1993-09-07
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Granted: 1996-09-03
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Expires: 2013-09-07
Abstract
Abstract Methods for detecting target DNA's in a sample on the basis of oscillation frequency using nucleic acid sequences immobilized to the surface of an electrode of an elastic wave element, and DNA sensors for conducting the methods are described. These methods provide for highly sensitive detection of target DNA's in a sample.
Inventors
- 👤 Noboru Oyama
- 👤 Shuichiro Yamaguchi
- 👤 Takeshi Shimomura
- 👤 Keizaburo Miki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:01
CPC Classifications
G; G01; G01N; G01N29/00; G01N29/04; G01N29/12; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; G01N2291/00; G01N2291/02; G01N2291/024; G01N2291/02466; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
247 words
A method for detecting a target DNA in a sample comprising the following steps: (i) immobilizing a double stranded nucleic acid sequence to the surface of an electrode of an elastic wave element, wherein said double stranded nucleic acid sequence comprises a first nucleic acid sequence having a 5'-terminus which is bound to the surface of the electrode of the elastic wave element, and a second nucleic acid sequence which is complementary to the first nucleic acid sequence which is hybridized to the first sequence and wherein the 5'-terminus of said second nucleic acid sequence is bound to a chemical species of sufficient molecular weight to produce a nucleic acid sequence having a higher mass than a target DNA which is to be detected; (ii) contacting said immobilized nucleic acid sequence with a sample suspected of containing a target DNA sequence which is complementary to the first nucleic acid sequence under conditions that provide for the displacement of the second nucleic acid sequence from the first nucleic acid sequence and the hybridization of the target DNA sequence to the first nucleic acid sequence; and (iii) detecting the presence of the target DNA sequence in the sample on the basis of an increase in oscillation frequency caused by the reduction in mass of the immobilized double stranded nucleic acid sequence which occurs upon displacement of the second nucleic acid sequence by the target DNA sequence and the subsequent hybridization of the target DNA to the first DNA sequence.
Claim 2
Independent Claim
247 words
A method for detecting a target DNA in a sample comprising the following steps: (i) immobilizing a double stranded nucleic acid sequence to the surface of an electrode of an elastic wave element, wherein said double stranded nucleic acid sequence comprises a first nucleic acid sequence having a 5'-terminus which is bound to the surface of the electrode of the elastic wave element, and a second nucleic acid sequence which is complementary to the first nucleic acid sequence which is hybridized to the first sequence and wherein the 5'-terminus of said second nucleic acid sequence is bound to a chemical species of sufficient molecular weight to produce a nucleic acid sequence having a higher mass than a target DNA which is to be detected; (ii) contacting said immobilized nucleic acid sequence with a sample suspected of containing a target DNA sequence which is complementary to the first nucleic acid sequence under conditions that provide for the displacement of the second nucleic acid sequence from the first nucleic acid sequence and the hybridization of the target DNA sequence to the first nucleic acid sequence; and (iii) detecting the presence of the target DNA sequence in the sample on the basis of an increase in oscillation frequency caused by the reduction in mass of the immobilized double stranded nucleic acid sequence which occurs upon displacement of the second nucleic acid sequence by the target DNA sequence and the subsequent hybridization of the target DNA to the first DNA sequence.
Claim 3
Dependent Claim
21 words
A method according to claim 1, wherein said elastic wave element is a quartz oscillator or a surface elastic wave element.
Claim 4
Dependent Claim
21 words
A method according to claim 1, wherein said chemical species has mass in the range of 0.01 pg to 1 ng.
Claim 5
Dependent Claim
22 words
A method according to claim 1, wherein said chemical species has a specific gravity of not less than 1 g/cm 3 .
Claim 6
Dependent Claim
21 words
A method according to claim 1, wherein said chemical species has a diameter in the range of 0.01 to 10 μm.
Claim 7
Dependent Claim
29 words
A method according to claim 1, wherein said chemical species is at least one member selected from the group consisting of synthetic resins, magnetic materials, metals, and metal oxides.
Claim 8
Dependent Claim
15 words
A method according to claim 6, wherein said synthetic resin is polystyrene of fluorine resin.
Claim 9
Dependent Claim
18 words
A method according to claim 6, wherein said metal is gold or said metal oxide is titanium oxide.
Claim 10
Dependent Claim
13 words
A method according to claim 6, wherein said magnetic material is a ferrite.
Claim 11
Dependent Claim
140 words
A DNA sensor which provides for the detection of a target DNA sequence comprising a double stranded nucleic acid sequence which is immobilized to the surface of an electrode of an elastic wave element, wherein said double stranded nucleic acid sequence comprises a first nucleic acid sequence having a 5'-terminus which is bound to the surface of the elastic wave element, and a second nucleic acid sequence complementary thereto, which is not said target DNA sequence and which is hybridized to the first nucleic acid sequence and wherein the 5' terminal end of said second nucleic acid sequence is bound to a chemical species of sufficient molecular weight to produce a nucleic acid sequence having a greater mass than a target DNA complementary to the first DNA sequence which is to be detected on the basis of increased oscillation frequency.
Claim 12
Dependent Claim
140 words
A DNA sensor which provides for the detection of a target DNA sequence comprising a double stranded nucleic acid sequence which is immobilized to the surface of an electrode of an elastic wave element, wherein said double stranded nucleic acid sequence comprises a first nucleic acid sequence having a 5'-terminus which is bound to the surface of the elastic wave element, and a second nucleic acid sequence complementary thereto, which is not said target DNA sequence and which is hybridized to the first nucleic acid sequence and wherein the 5' terminal end of said second nucleic acid sequence is bound to a chemical species of sufficient molecular weight to produce a nucleic acid sequence having a greater mass than a target DNA complementary to the first DNA sequence which is to be detected on the basis of increased oscillation frequency.
Claim 13
Dependent Claim
22 words
A DNA sensor according to claim 10, wherein said elastic wave element is a quartz oscillator or a surface elastic wave element.
Claim 14
Dependent Claim
23 words
A DNA sensor according to claim 10, wherein said chemical species has a mass in the range of 0.01 pg to 1 ng.
Claim 15
Dependent Claim
23 words
A DNA sensor according to claim 10, wherein said chemical species has a specific gravity of not less than 1 g/cm 3 .
Claim 16
Dependent Claim
22 words
A DNA sensor according to claim 10, wherein said chemical species has a diameter in the range of 0.01 to 10 μm.
Claim 17
Dependent Claim
30 words
A DNA sensor according to claim 10, wherein said chemical species is at least one member selected from the group consisting of synthetic resins, magnetic materials, metals, and metal oxides.
Claim 18
Dependent Claim
16 words
A DNA sensor according to claim 15, wherein said synthetic resin is polystyrene or fluorine resin.
Claim 19
Dependent Claim
19 words
A DNA sensor according to claim 15, wherein said metal is gold or said metal oxide is titanium oxide.
Claim 20
Dependent Claim
14 words
A DNA sensor according to claim 15, wherein said magnetic material is a ferrite.
References Cited (Backward Citations)
US4766062A
Cited
US4725537A
Cited
US4752566A
Cited
US4818680A
Cited
EP0295965A2
Cited
US4847193A
Cited
WO1989009938A1
Cited
JPH0354467A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-09-03 | 2000-03-04 | 2000-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-09-03 | 2004-03-04 | 2004-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-09-03 | 2008-03-04 | 2008-09-03 | $7700.00 | Window Closed / Lapsed |