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US5523286A
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Stroma-derived proteoglycan containing composition which promotes differentiation and maintains the self-renewal capacity of long-term bone marrow culture-initiating cells
Assignee: University of Minnesota System
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Filing: 1993-11-12
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Granted: 1996-06-04
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Expires: 2013-11-12
Abstract
Abstract An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD.
Inventors
- 👤 Philip B. McGlave
- 👤 Catherine M. Verfaillie
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 02:01
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4725; C07K14/76; C07K14/77; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0647; C12N2501/00; C12N2501/10; C12N2501/125; C12N2501/20; C12N2501/22; C12N2501/23; C12N2501/2306; C12N2501/235; C12N2501/90; C12N2501/998; C12N2502/00; C12N2502/13; C12N2502/1394
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
123 words
A composition, capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture-initiating cells in a mammalian hematopoietic cell culture, said composition prepared by a process comprising: subjecting an aqueous culture medium conditioned by stromal cells to ion exchange chromatography to obtain a fraction comprising two anionic proteoglycans; wherein one of said two proteoglycans has a molecular weight of greater than 200 kD as measured by SDS-PAGE under reducing conditions, and comprises a core protein and a polysaccharide portion which comprises chondroitin sulfate; and wherein the other of said two proteoglycans has a molecular weight of greater than 200 kD as measured by SDS-PAGE under reducing conditions, and comprises a core protein and a polysaccharide portion which comprises heparan sulfate.
Claim 2
Dependent Claim
123 words
A composition, capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture-initiating cells in a mammalian hematopoietic cell culture, said composition prepared by a process comprising: subjecting an aqueous culture medium conditioned by stromal cells to ion exchange chromatography to obtain a fraction comprising two anionic proteoglycans; wherein one of said two proteoglycans has a molecular weight of greater than 200 kD as measured by SDS-PAGE under reducing conditions, and comprises a core protein and a polysaccharide portion which comprises chondroitin sulfate; and wherein the other of said two proteoglycans has a molecular weight of greater than 200 kD as measured by SDS-PAGE under reducing conditions, and comprises a core protein and a polysaccharide portion which comprises heparan sulfate.
Claim 3
Dependent Claim
24 words
The composition of claim 1 wherein said core protein, of the proteoglycan which comprises chondroitin sulfate, has a molecular weight of about 90 kD.
Claim 4
Dependent Claim
24 words
The composition of claim 1 wherein said core protein, of the proteoglycan which comprises heparan sulfate, has a molecular weight of about 65 kD.
Claim 5
Dependent Claim
57 words
A proteoglycan having a molecular weight of greater than about 200 kD, comprising: a core protein having a molecular weight of about 65 kD, and a polysaccharide portion which comprises heparan sulfate; wherein said proteoglycan is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture-initiating cells in a mammalian hematopoietic cell culture.
Claim 6
Dependent Claim
57 words
A proteoglycan having a molecular weight of greater than about 200 kD, comprising: a core protein having a molecular weight of about 65 kD, and a polysaccharide portion which comprises heparan sulfate; wherein said proteoglycan is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture-initiating cells in a mammalian hematopoietic cell culture.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-06-04 | 1999-12-04 | 2000-06-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-06-04 | 2003-12-04 | 2004-06-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-06-04 | 2007-12-04 | 2008-06-04 | $7700.00 | Window Closed / Lapsed |