US5494639A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor for measuring changes in viscosity and/or density of a fluid
Assignee: Hoechst AG
•
Filing: 1994-10-13
•
Granted: 1996-02-27
•
Expires: 2014-10-13
Abstract
Abstract A disposable biosensor for measuring changes in viscosity and/or density in a test fluid includes a disposable housing surrounding a measuring chamber. A piezoelectric element is disposed in the measuring chamber and constitutes an oscillating unit. The piezoelectric element has a measuring surface adapted to be wetted with a measuring mixture comprising the test fluid and a reaction component. The reaction component is disposed inside the measuring chamber in a vicinity of and not touching the measuring surface of the piezoelectric element before any test fluid is introduced into the measuring chamber. Access is provided in the disposable housing for introducing the test fluid into the measuring chamber so that the test fluid comes into contact with the reaction component and the measuring surface of the piezoelectric element upon its introduction into the measuring chamber.
Inventors
- 👤 Andrzej Grzegorzewski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 12:08
CPC Classifications
G; G01; G01N; G01N29/00; G01N29/02; G01N29/022; G01N11/00; G01N11/10; G01N11/16; G01N29/22; G01N29/222; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/4905; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N9/00; G01N9/002; G01N2291/00; G01N2291/02; G01N2291/028; G01N2291/02818
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
143 words
A disposable biosensor for measuring changes in at least one of viscosity and density in a test fluid, including: a disposable housing; a measuring chamber surrounded by the disposable housing; a piezoelectric element disposed in the measuring chamber and constituting an oscillating unit, the piezoelectric element having a measuring surface adapted to be wetted with a measuring mixture comprising the test fluid and a reaction component; means for disposing the reaction component inside the measuring chamber in a vicinity of and not touching the measuring surface of the piezoelectric element before any test fluid is introduced into the measuring chamber; and access means in said disposable housing for introducing the test fluid into the measuring chamber so that the test fluid comes into contact with the reaction component and the measuring surface of the piezoelectric element upon its introduction into the measuring chamber.
Claim 2
Dependent Claim
143 words
A disposable biosensor for measuring changes in at least one of viscosity and density in a test fluid, including: a disposable housing; a measuring chamber surrounded by the disposable housing; a piezoelectric element disposed in the measuring chamber and constituting an oscillating unit, the piezoelectric element having a measuring surface adapted to be wetted with a measuring mixture comprising the test fluid and a reaction component; means for disposing the reaction component inside the measuring chamber in a vicinity of and not touching the measuring surface of the piezoelectric element before any test fluid is introduced into the measuring chamber; and access means in said disposable housing for introducing the test fluid into the measuring chamber so that the test fluid comes into contact with the reaction component and the measuring surface of the piezoelectric element upon its introduction into the measuring chamber.
Claim 3
Dependent Claim
21 words
The biosensor according to claim 1, wherein the piezoelectric element is a quartz crystal operational in a shear mode of vibration.
Claim 4
Dependent Claim
19 words
The biosensor according to claim 1, wherein the disposable housing comprises a plurality of layers glued to one another.
Claim 5
Dependent Claim
111 words
The biosensor of claim 3, wherein the plurality of layers comprises five layers, including: a first layer forming a closed bottom for the housing; a second layer disposed above the first layer and leaving a free space under the piezoelectric element; a third layer disposed above the second layer and retaining the piezoelectric element; a fourth layer disposed above the third layer and forming the measuring chamber above the piezoelectric element; and a fifth layer disposed above the fourth layer and forming a lid for closing the measuring chamber above the piezoelectric element, the access means being disposed in the fifth layer for introducing the test fluid into the measuring chamber.
Claim 6
Dependent Claim
26 words
The biosensor according to claim 1, wherein the housing comprises two housing shells glued together and the piezoelectric element is inserted between the two housing shells.
Claim 7
Dependent Claim
55 words
The biosensor of claim 5, wherein a first one of the housing shells includes the access means and a recess for receiving the piezoelectric element, and a second one of the two housing shells forms a closed bottom for the housing and includes a protruding segment which rests on an edge of the piezoelectric element.
Claim 8
Dependent Claim
62 words
The biosensor of claim 1, wherein the disposing means comprises a substrate carrying the reaction component and the disposable housing comprises a one-piece housing member including an inner chamber and an introduction conduit communicating with the inner chamber, the substrate and the piezoelectric element being inserted into the inner chamber through the introduction conduit, the piezoelectric element being glued to the substrate.
Claim 9
Dependent Claim
15 words
The biosensor according to claim 1, wherein the housing is made of injection molded plastic.
Claim 10
Dependent Claim
18 words
The biosensor according to claim 1, wherein the access means comprises a bore communicating with the measuring chamber.
Claim 11
Dependent Claim
23 words
The biosensor according to claim 1, wherein the access means comprises a membrane covering the measuring chamber and permeable to the test fluid.
Claim 12
Dependent Claim
29 words
The biosensor according to claim 1, wherein the measuring chamber has walls to which the reaction component is applied, said walls with the reaction component constituting the disposing means.
Claim 13
Dependent Claim
35 words
The biosensor according to claim 1, wherein said disposing means includes a substrate containing the reaction component and adapted to be inserted into the measuring chamber such that it does not touch the measuring surface.
Claim 14
Dependent Claim
20 words
The biosensor according to claim 12, wherein the substrate comprises an absorbent pad of one of batting and filter paper.
Claim 15
Dependent Claim
18 words
The biosensor according to claim 12, wherein the substrate comprises a grid of one of plastic and metal.
Claim 16
Dependent Claim
48 words
The biosensor according to claim 1, wherein the housing includes a substrate board having conductor tracks printed thereon and terminal faces extending out of the housing, the piezoelectric element is mounted on the substrate board and is connected to the terminal faces by way of the conductor tracks.
Claim 17
Dependent Claim
26 words
The biosensor according to claim 1, wherein the biosensor is a blood coagulation sensor, and wherein the reaction component comprises a substance for influencing blood coagulation.
Claim 18
Dependent Claim
13 words
The biosensor according to claim 16, wherein the substance comprises CaCl 2 .
Claim 19
Dependent Claim
29 words
The biosensor according to claim 1, wherein the biosensor is a sensor for detecting antigen-antibody reactions, and wherein the reaction component comprises one of an antigen and an antibody.
Claim 20
Dependent Claim
23 words
The biosensor according to claim 1, and further including a temperature equalization device for keeping temperature of the biosensor at a constant value.
Claim 21
Dependent Claim
31 words
A biosensor system including the biosensor according to claim 1 and further including an electronic evaluation circuit coupled to said piezoelectric element for evaluating changes in parameters of the oscillating unit.
Claim 22
Dependent Claim
48 words
The biosensor system according to claim 20, wherein the electronic evaluation circuit comprises an oscillator circuit in which the piezoelectric element is connected for determining frequency of the oscillator circuit and a microprocessor circuit coupled to the oscillator circuit for measuring and digitally evaluating changes in the frequency.
Claim 23
Dependent Claim
34 words
The biosensor system according to claim 20, wherein the piezoelectric element has a temperature dependency and said electronic evaluation circuit measures temperature of the biosensor element utilizing the temperature dependency of the piezoelectric element.
References Cited (Backward Citations)
JPS4932767A
Cited
US4236893A
Cited
US4314821A
Cited
EP0177858B1
Cited
US4735906A
Cited
JPS62153761A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-02-26 | 1999-08-28 | 2000-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-02-26 | 2003-08-28 | 2004-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-26 | 2007-08-28 | 2008-02-27 | $7700.00 | Window Closed / Lapsed |