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US5494592A
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Apheresis apparatus and method
Assignee: Haemonetics Corp
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Filing: 1995-01-13
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Granted: 1996-02-27
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Expires: 2015-01-13
Abstract
Abstract An apheresis apparatus and method is disclosed for increasing the purity and yield of platelets separated from donated whole blood in a centrifuge. The whole blood in the centrifuge is diluted by recirculating fluid, such as plasma or saline, at a first flow rate, to mix with further withdrawn whole blood prior to entering the centrifuge. As plasma is collected, it is recirculated through the centrifuge at a second flow rate to further improve the separation between the intermediate density components, i.e., platelets and white blood cells in the "buffy coat." The plasma is then recirculated through the centrifuge at a third flow rate and platelets are displaced out of the centrifuge while the plasma is recirculating through the centrifuge at the third flow rate.
Inventors
- 👤 Allen Latham, Jr.
- 👤 Glen E. Jorgensen
- 👤 Theodoor H. S. Sibinga
- 👤 Joseph R. Plante
- 👤 Tracey E. Knapp
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 01:06
CPC Classifications
A; A61; A61M; A61M1/00; A61M1/36; A61M1/3693; A61M1/14; A61M1/30; A61M1/301; A61M1/304; A61M1/3601; A61M1/3603; A61M1/3696; A61M2205/00; A61M2205/33; A61M2205/3306; A61M2205/331; B; B04; B04B; B04B5/00; B04B5/04; B04B5/0442; B04B2005/0464; B04B2005/0471
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
184 words
A method of increasing intermediate density blood component yield from donated whole blood in a centrifugation fractionation volume, the intermediate density component consisting primarily of at least one of a platelet constituent and a white blood cell constituent, the method comprising the steps of: (i) diluting whole blood, by mixing fluid with the whole blood at a first flow rate as the blood is introduced into the volume; (ii) separating lower density component in the volume from higher density components in the volume including said intermediate density component, and displacing said lower density component to a first container; (iii) recirculating the lower density component to the volume at a second, substantially constant flow rate to further dilute the components remaining in the volume and widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a third, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating through the volume at the third flow rate.
Claim 2
Independent Claim
184 words
A method of increasing intermediate density blood component yield from donated whole blood in a centrifugation fractionation volume, the intermediate density component consisting primarily of at least one of a platelet constituent and a white blood cell constituent, the method comprising the steps of: (i) diluting whole blood, by mixing fluid with the whole blood at a first flow rate as the blood is introduced into the volume; (ii) separating lower density component in the volume from higher density components in the volume including said intermediate density component, and displacing said lower density component to a first container; (iii) recirculating the lower density component to the volume at a second, substantially constant flow rate to further dilute the components remaining in the volume and widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a third, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating through the volume at the third flow rate.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the components remaining in the bowl after (v) are reinfused.
Claim 4
Dependent Claim
17 words
The method of claim 1 wherein the second flow rate is greater than the first flow rate.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the third flow rate is greater than the second flow rate.
Claim 6
Dependent Claim
16 words
The method of claim 1 further comprising collecting the intermediate density component in a second container.
Claim 7
Dependent Claim
18 words
The method of claim 5 further comprising anticoagulating the whole blood with anticoagulant stored in a third container.
Claim 8
Dependent Claim
37 words
The method of claim 6 wherein said intermediate density component consists primarily of platelets and wherein the method further comprises displacing white blood cells from the volume and collecting the white blood cells in a fourth container.
Claim 9
Dependent Claim
15 words
The method of claim 1 in which said intermediate density component consists primarily of platelets.
Claim 10
Dependent Claim
16 words
The method of claim 1 wherein said intermediate density component consists primarily of white blood cells.
Claim 11
Dependent Claim
18 words
The method of claim 1 wherein said intermediate density component consists primarily of platelets and white blood cells.
Claim 12
Independent Claim
174 words
A method of increasing intermediate density component yield from donated whole blood in a centrifuge having an enclosed fractionation volume, the intermediate density component consisting primarily of at least one of a platelet constituent and a white blood cell constituent, the method comprising: (i) pumping whole blood into the volume along a first flow route; (ii) separating lower density component in the volume from higher density components in the volume including said intermediate density component, and displacing the lower density component to a first container; (iii) closing the first flow route and opening a second flow route, and returning the lower density component to the volume at a first, substantially constant flow rate to widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a second, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating though the volume at the second flow rate.
Claim 13
Independent Claim
174 words
A method of increasing intermediate density component yield from donated whole blood in a centrifuge having an enclosed fractionation volume, the intermediate density component consisting primarily of at least one of a platelet constituent and a white blood cell constituent, the method comprising: (i) pumping whole blood into the volume along a first flow route; (ii) separating lower density component in the volume from higher density components in the volume including said intermediate density component, and displacing the lower density component to a first container; (iii) closing the first flow route and opening a second flow route, and returning the lower density component to the volume at a first, substantially constant flow rate to widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a second, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating though the volume at the second flow rate.
Claim 14
Dependent Claim
16 words
The method of claim 11 wherein the components remaining in the bowl after (v) are reinfused.
Claim 15
Dependent Claim
28 words
The method of claim 11 wherein the second flow rate is greater than the first flow rate over an entire range of acceleration of the second flow rate.
Claim 16
Dependent Claim
16 words
The method of claim 11 further comprising collecting the intermediate density component in a second container.
Claim 17
Dependent Claim
18 words
The method of claim 14 further comprising anticoagulating the whole blood with anticoagulant stored in a third container.
Claim 18
Dependent Claim
35 words
The method of claim 15 wherein the intermediate density component comprises primarily platelets and the method further comprises displacing white blood cells from the volume and collecting the white blood cells in a fourth container.
Claim 19
Dependent Claim
15 words
The method of claim 11 in which said intermediate density component consists primarily of platelets.
Claim 20
Dependent Claim
32 words
The method of claim 11 wherein the lower density component initially returns to the volume at a flow rate which exceeds the first flow rate before slowing to the first flow rate.
Claim 21
Dependent Claim
16 words
The method of claim 11 wherein said intermediate density component consists primarily of white blood cells.
Claim 22
Dependent Claim
18 words
The method of claim 11 wherein said intermediate density component consists primarily of platelets and white blood cells.
Claim 23
Independent Claim
170 words
A method of increasing component yield from donated whole blood in a centrifuge having an enclosed fractionation volume, the method comprising: (i) pumping into the volume anticoagulated whole blood having components of varying density including an intermediate density component which consists primarily of at least one of a platelet constituent and a white blood cell constituent; (ii) separating lower density component in the volume from higher density components including said intermediate density component in the volume and displacing the lower density component to a first container; (iii) returning the lower density component to the volume at a first, substantially constant flow rate to dilute components remaining in the volume and widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a second, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating through the volume at the second flow rate.
Claim 24
Independent Claim
170 words
A method of increasing component yield from donated whole blood in a centrifuge having an enclosed fractionation volume, the method comprising: (i) pumping into the volume anticoagulated whole blood having components of varying density including an intermediate density component which consists primarily of at least one of a platelet constituent and a white blood cell constituent; (ii) separating lower density component in the volume from higher density components including said intermediate density component in the volume and displacing the lower density component to a first container; (iii) returning the lower density component to the volume at a first, substantially constant flow rate to dilute components remaining in the volume and widen the region occupied by said intermediate density component without causing said intermediate density component to exit the fractionation volume; (iv) recirculating the lower density component through the volume at a second, accelerating flow rate; and (v) displacing said intermediate density component from the volume while the lower density component is recirculating through the volume at the second flow rate.
Claim 25
Dependent Claim
16 words
The method of claim 21 wherein the components remaining in the bowl after (v) are reinfused.
Claim 26
Dependent Claim
16 words
The method of claim 21 further comprising collecting said intermediate density component in a second container.
Claim 27
Dependent Claim
18 words
The method of claim 23 further comprising anticoagulating the whole blood with anticoagulant stored in a third container.
Claim 28
Dependent Claim
36 words
The method of claim 24 wherein said intermediate density component consists primarily of platelets and the method further comprises displacing white blood cells from the volume and collecting the white blood cells in a fourth container.
Claim 29
Dependent Claim
14 words
The method of claim 24 wherein said intermediate density component consists primarily of platelets.
Claim 30
Dependent Claim
32 words
The method of claim 24 wherein the lower density component initially returns to the volume at a flow rate which exceeds the first flow rate before slowing to the first flow rate.
Claim 31
Dependent Claim
35 words
The method of claim 24 wherein the whole blood is diluted with lower density component recirculating at a low flow rate before reaching the volume, the low rate being less than the first flow rate.
Claim 32
Dependent Claim
16 words
The method of claim 21 wherein said intermediate density component consists primarily of white blood cells.
Claim 33
Dependent Claim
21 words
The method of claim 21 wherein said intermediate density component consists primarily of platelets and white blood cells. hite blood cells.
References Cited (Backward Citations)
US3145713A
Cited
US3957197A
Cited
US4416654A
Cited
US4464167A
Cited
US4675117A
Cited
EP0155684B1
Cited
US5141645A
Cited
WO1987006472A1
Cited
US4990132A
Cited
US5370802A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-02-26 | 1999-08-28 | 2000-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-02-26 | 2003-08-28 | 2004-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-02-26 | 2007-08-28 | 2008-02-27 | $7700.00 | Window Closed / Lapsed |