US5474935A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Adeno-associated virus (AAV)-based eucaryotic vectors
Assignee: United States Department of Health and Human Services
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Filing: 1993-11-03
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Granted: 1995-12-12
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Expires: 2013-11-03
Abstract
Abstract The present invention relates to adeno-associated virus (AAV)-based eucaryotic vectors and uses thereof. Such vectors may, for example, be used to down regulate any targeted viral or cellular gene whose sequence is known. Furthermore, the vectors may also be used to cause the expression of proteins.
Inventors
- 👤 Saswati Chatterjee
- 👤 K. K. Wong, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:35
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1131; C12N15/1132; C07; C07K; C07K14/00; C07K14/005; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61K; A61K38/00; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16311; C12N2740/16322; C12N2750/00; C12N2750/00011; C12N2750/14011; C12N2750/14111; C12N2750/14141; C12N2750/14143
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
59 words
An AAV-based vector that is suitable for transferring a heterologous DNA fragment into cells in vitro, comprising: (a) vector pWC3, (b) a heterologous promoter oriented so as to promote transcription towards the AAV polyadenylation signal of said pWC3 vector, and (c) a heterologous DNA fragment, wherein said heterologous DNA fragment is under the regulatory control of said heterologous promoter.
Claim 2
Dependent Claim
59 words
An AAV-based vector that is suitable for transferring a heterologous DNA fragment into cells in vitro, comprising: (a) vector pWC3, (b) a heterologous promoter oriented so as to promote transcription towards the AAV polyadenylation signal of said pWC3 vector, and (c) a heterologous DNA fragment, wherein said heterologous DNA fragment is under the regulatory control of said heterologous promoter.
Claim 3
Dependent Claim
25 words
An AAV-based vector according to claim 1, wherein said heterologous DNA fragment encodes an antisense polynucleotide sequence that is complementary to a targeted RNA sequence.
Claim 4
Dependent Claim
21 words
An AAV-based vector according to claim 1, wherein said heterologous DNA fragment is a gene that encodes a protein of interest.
Claim 5
Dependent Claim
80 words
An AAV-based vector according to claim 2, further comprising: (d) a second heterologous promoter oriented so as to promote transcription in a direction opposite to said heterologous promoter of (b), and (e) a second heterologous DNA fragment, wherein said second heterologous DNA fragment is either a gene that encodes a selectable marker or is a gene that encodes an enzymatically measurable marker protein, and wherein said second heterologous DNA fragment is under the regulatory control of said second heterologous promoter.
Claim 6
Dependent Claim
18 words
An AAV-based vector according to claim 4, wherein said targeted RNA sequence is ICP4 of herpes simplex virus.
Claim 7
Dependent Claim
16 words
An AAV-based vector according to claim 4, wherein said targeted RNA sequence is E1a of adenovirus.
Claim 8
Dependent Claim
15 words
An AAV-based vector according to claim 4, wherein said targeted RNA sequence is HIV-1 TAR.
Claim 9
Dependent Claim
12 words
An AAV-based vector according to claim 7, wherein said vector is pCWRSV-HIVAlpha-Neo.
Claim 10
Dependent Claim
17 words
An AAV-based vector according to claim 1, wherein said heterologous promoter is the Rous sarcoma virus promoter.
Claim 11
Dependent Claim
6 words
An AAV-based vector which is pWC3.
Claim 12
Dependent Claim
6 words
An AAV-based vector which is pWC3.
Claim 13
Dependent Claim
6 words
An AAV-based vector which is pCWRSV.
Claim 14
Dependent Claim
6 words
An AAV-based vector which is pCWRSV.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-12-11 | 1999-06-12 | 1999-12-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-12-11 | 2003-06-12 | 2003-12-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-12-11 | 2007-06-12 | 2007-12-12 | $7700.00 | Window Closed / Lapsed |