US5470739A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture support having patterned substance that influences cell adhesion
Assignee: NEC Corp
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Filing: 1992-12-22
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Granted: 1995-11-28
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Expires: 2012-12-22
Abstract
Abstract The invention discloses a cell culture support which provides for the adhesion and culturing of one or more adhesive cells using a photoresist in which to provide a particular patterned design on a surface of the support. The patterned design is provided by the photoresist which is partially removed by photolithography during the making of the support which in turn imparts a striped, checkerboard or dotted pattern on the surface of the support. Further, the cell culture support is produced by pretreating the support surface with a reagent to provide hydrophobicity to the support surface. Also a reagent can be added to pretreat the support surface in order to facilitate adhesion at the photoresist prior to applying the photoresist into the cell culture support. Collagen is applied in the form of a solution, containing in addition thereto albumin and a crosslinking agent, in order to form a film. Collagen specifically affects the cell adhesion rate or the morphology of the cells to be adhered to the support. The steps of applying a photoresist, partially removing it, providing hydrophobicity to the cell support surface, applying a solution to affect cell adhesiveness, film formation and dissolving the remaining photoresist are carried out repeatedly. In addition using a solution to promote film formation of a different film composition and thickness than the first is applied to produce the cell culture support. By varying the film composition a patterned design, such as checker board, is developed for purposes of providing a cell culture support for adherent cells.
Inventors
- 👤 Toshihiro Akaike
- 👤 Seishiro Tobe
- 👤 Shigeyuki Miyamoto
- 👤 Akio Ohashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 17:22
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12M; C12M25/00; C12M25/06; C12M25/08; C12N2535/00; C12N2535/10
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
91 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) forming a crosslinked collagen film on the support surface from (ii); and (iv) dissolving the photoresist remaining on the support surface from (iii) to produce said cell culture support.
Claim 2
Dependent Claim
91 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) forming a crosslinked collagen film on the support surface from (ii); and (iv) dissolving the photoresist remaining on the support surface from (iii) to produce said cell culture support.
Claim 3
Dependent Claim
56 words
The cell culture support of claim 1 wherein the partial removal of the photoresist by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing a irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface.
Claim 4
Dependent Claim
35 words
The cell culture support of claim 1 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface.
Claim 5
Dependent Claim
29 words
The cell culture support of claim 1 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface.
Claim 6
Dependent Claim
30 words
The cell culture support of claim 1 wherein the photoresist layer is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water.
Claim 7
Dependent Claim
14 words
The cell culture support of claim 1 wherein the support contains cells adhered thereto.
Claim 8
Dependent Claim
115 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) forming a crosslinked collagen film on the support surface from (iii); (iv) dissolving the photoresist remaining on the support surface from (iii); and (v) repeating the foregoing sequence of steps and in repeated step (iii) forming a film different in composition or thickness from the collagent film to produce said cell culture support.
Claim 9
Dependent Claim
115 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) forming a crosslinked collagen film on the support surface from (iii); (iv) dissolving the photoresist remaining on the support surface from (iii); and (v) repeating the foregoing sequence of steps and in repeated step (iii) forming a film different in composition or thickness from the collagent film to produce said cell culture support.
Claim 10
Dependent Claim
56 words
The cell culture support of claim 7 wherein the partial removal of the photoresist by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing the irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface.
Claim 11
Dependent Claim
35 words
The cell culture support of claim 7 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface.
Claim 12
Dependent Claim
29 words
The cell culture support of claim 7 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface.
Claim 13
Dependent Claim
30 words
The cell culture support of claim 7 wherein the photoresist layer is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water.
Claim 14
Dependent Claim
106 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereto which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions providing for film formation; and (iv) dissolving the photoresist remaining on the cell culture support surface to produce said cell culture support.
Claim 15
Dependent Claim
106 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereto which is produced by a process comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent that imparts hydrophobicity to the support surface; (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions providing for film formation; and (iv) dissolving the photoresist remaining on the cell culture support surface to produce said cell culture support.
Claim 16
Dependent Claim
57 words
The cell culture support of claim 12 wherein the partial removal of the photoresist layer by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing the irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface.
Claim 17
Dependent Claim
35 words
The cell culture support of claim 12 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface.
Claim 18
Dependent Claim
29 words
The cell culture support of claim 12 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface.
Claim 19
Dependent Claim
29 words
The cell culture support of claim 12 wherein the photoresist is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water.
Claim 20
Dependent Claim
139 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereon which support is produced by a method comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent which imparts hydrophobicity to the support surface; (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions which provide for film formation; (iv) dissolving the photoresist remaining on the cell culture support surface; and (v) repeating the foregoing sequence of steps and in repeated step (iii) using a solution which forms a film of a different composition or thickness from the film in step (iii) to produce said cell culture support.
Claim 21
Dependent Claim
139 words
A cell culture support which provides for the adhesion and culturing of one or more types of adhesive cells thereon which support is produced by a method comprising: (i) applying a photoresist onto a cell culture support surface and then partially removing the photoresist by photolithography; (ii) pretreating the support surface from (i) with a reagent which imparts hydrophobicity to the support surface; (iii) applying a solution comprising (a) collagen, (b) albumin, and (c) a crosslinking agent to the cell culture support surface under conditions which provide for film formation; (iv) dissolving the photoresist remaining on the cell culture support surface; and (v) repeating the foregoing sequence of steps and in repeated step (iii) using a solution which forms a film of a different composition or thickness from the film in step (iii) to produce said cell culture support.
Claim 22
Dependent Claim
56 words
The cell culture support of claim 17 wherein the partial removal of the photoresist by photolithography is effected by treatment of the photoresist on the cell culture support surface by irradiation through a photomask and developing the irradiated photoresist under conditions which result in a predetermined patterning of the photoresist on the cell culture support surface.
Claim 23
Dependent Claim
35 words
The cell culture support of claim 17 wherein the cell culture support surface is pretreated with a reagent which facilitates adhesion at the photoresist prior to applying the photoresist into the cell culture support surface.
Claim 24
Dependent Claim
29 words
The cell culture support of claim 17 wherein the photoresist is removed by photolithography to provide for a striped, checkerboard or dotted pattern on the cell culture support surface.
Claim 25
Dependent Claim
29 words
The cell culture support of claim 17 wherein the photoresist is dissolved by treatment with an organic solvent and the cell culture support surface is then washed with water.-
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-11-27 | 1999-05-29 | 1999-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-11-27 | 2003-05-29 | 2003-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-11-27 | 2007-05-29 | 2007-11-28 | $7700.00 | Window Closed / Lapsed |