Patent US5470726A successfully refreshed from Google Patents.
US5470726A
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Retrovirus packaging and producer cell lines based on gibbon ape leukemia virus
Assignee: Fred Hutchinson Cancer Center
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Filing: 1993-08-11
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Granted: 1995-11-28
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Expires: 2013-08-11
Abstract
Abstract Retrovirus packaging cell lines PG13 (ATCC No. CRL 10686) and PG13/LNc8 (ATCC No. CRL 10685), and retroviruses packaged by said cells.
Inventors
- 👤 A. Dusty Miller
- 👤 Jose V. Garcia-Martinez
- 👤 Maribeth V. Eiden
- 👤 Carolyn A. Wilson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:46
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13051; C12N2740/13052
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
10 words
A retrovirus packaging cell line PG13 (ATCC No. CRL 10686).
Claim 2
Dependent Claim
10 words
A retrovirus packaging cell line PG13 (ATCC No. CRL 10686).
Claim 3
Dependent Claim
49 words
A process for generating a producer cell line useful for producing a retroviral vector for transferring a gene of interest into recipient mammalian cells, comprising infecting cells of the cell line of claim 1 with a vector plasmid which comprises a psi packaging sequence and a gene of interest.
Claim 4
Dependent Claim
11 words
A producer cell line produced by the process of claim 2.
Claim 5
Dependent Claim
19 words
Cell line PG13/LNc8 (ATCC No. CRL 10685) according to claim 3, wherein the gene of interest encodes neomycin phosphotransferase.
References Cited (Backward Citations)
EP0414035A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-11-27 | 1999-05-29 | 1999-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-11-27 | 2003-05-29 | 2003-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-11-27 | 2007-05-29 | 2007-11-28 | $7700.00 | Window Closed / Lapsed |