Patent US5462989A successfully refreshed from Google Patents.
US5462989A
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Fiber optic potassium ion sensor
Assignee: Sanofi Aventis Deutschland GmbH
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Filing: 1992-07-22
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Granted: 1995-10-31
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Expires: 2012-07-22
Abstract
Abstract A sensor (10) is provided for detecting the concentration of potassium ions which comprises a molecule which selectively complexes potassium ions. The molecule, e.g., 2,2-bis[3,4-(15-crown-5)-2-nitrophenylcarbamoxymethyl]tetradecanol-14, has at least one binding site and is provided with a fluorophore group, e.g., Rhodamine-B, at that site. The molecule is one that expands upon complexation of potassium ions, such that the intensity of fluorescence increases. The change in fluorescent intensity is a direct measure of the concentration of potassium ions. A detector employing the sensor is also provided.
Inventors
- 👤 Ganapati R. Mauze
- 👤 Lothar Rupp
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 23:52
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N21/643; C; C09; C09K; C09K11/00; C09K11/06; G01N31/00; G01N31/22; G01N33/00; G01N33/48; G01N33/50; G01N33/84; G01N21/645; G01N2021/6484; G01N21/75; G01N21/77; G01N21/7703; G01N2021/7706; G01N2021/772; G01N2021/7769; G01N2021/7786; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
77 words
A process for immobilizing a molecule having a fluorophore thereon on a hydrophilic polymer, said molecule comprising an ionophore having selectivity substantially limited to potassium ions, said ionophore selected from the group consisting of 2,2-bis[3,4-(15-crown-5)-2-nitrophenylcarboxymethyl]tetradecane, Val-inomycin, 18Crown-6, and derivatives thereof, and said fluorohore selected from the group consisting of Rhodamine-B, Rhodamine-110, Fluorescein, and 2,7'-dichlorofluoroscein, comprising forming a solution of said molecule in a monomer and initiating a polymerization reaction to form a gel comprising said hydrophilic polymer.
Claim 2
Dependent Claim
77 words
A process for immobilizing a molecule having a fluorophore thereon on a hydrophilic polymer, said molecule comprising an ionophore having selectivity substantially limited to potassium ions, said ionophore selected from the group consisting of 2,2-bis[3,4-(15-crown-5)-2-nitrophenylcarboxymethyl]tetradecane, Val-inomycin, 18Crown-6, and derivatives thereof, and said fluorohore selected from the group consisting of Rhodamine-B, Rhodamine-110, Fluorescein, and 2,7'-dichlorofluoroscein, comprising forming a solution of said molecule in a monomer and initiating a polymerization reaction to form a gel comprising said hydrophilic polymer.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein said molecule consists essentially of 2,2-bis[3,4-(15-crown-5)-2-nitrophenylcarbamoxymethyl]tetradecane.
Claim 4
Dependent Claim
13 words
The process of claim 1 wherein said fluorophore group consists essentially of Rhodamine-B.
Claim 5
Dependent Claim
25 words
The process of claim 1 wherein said molecule has an aliphatic part having a free end and said fluorophore is located on said free end.
Claim 6
Dependent Claim
73 words
The process of claim 1 wherein said molecule consists essentially of 2,2-bis[3,4-(15-crown-5)-2-nitrophenylcarbamoxymethyl]tetradecane and said fluorophore group consists essentially of Rhodamine-B, located on the fourteenth carbon atom of the tetradecane chain and is first dissolved in isopropanol to form a first solution, said solution is then added to a second solution of acrylamide and N,N'-methylenediacrylamide in water, and peroxidisulfate is added thereto to initiate polymerization, which is allowed to proceed to form said gel.
References Cited (Backward Citations)
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