US5462874A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dialyzed multiple well tissue culture plate
Assignee: Wheaton Industries Inc
•
Filing: 1993-06-23
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Granted: 1995-10-31
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Expires: 2013-06-23
Abstract
Abstract A multiple well tissue culture plate (10) having a plurality of opened bottomed wells (36) with a semi-permeable membrane (20) across the bottoms of a plurality of such wells (36). The multiple well tissue culture plate (10) is placed in contact with the basal medium (26) contained within the basal medium reservoir (12) to allow for exchange of nutrients and waste products between the basal medium (26) and the culture medium (22) across the semi-permeable membrane (20). The dialyzed multiple well tissue culture plate (50) provides dialysis of each individual well within the multiple well tissue culture plate (10) thus eliminating traditional exchange of culture medium to maintain proper culture conditions.
Inventors
- 👤 Martin L. Wolf
- 👤 John R. Wilson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:26
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/08; C12M23/12; C12M29/00; C12M29/04; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
133 words
A multiple well tissue culture assembly comprising a) a plate comprising a plurality of wells each open at the top and bottom and defining a first predetermined volume, b) a membrane closing off the bottom of each well, said membrane excluding passage therethrough of molecules in excess of a predetermined size between 10,000 and 50,000 daltons, c) a common reservoir defining a second predetermined volume greater than the sum of the first predetermined volumes of a plurality of said wells, said common reservoir having an access port for adding basal medium to and removing basal medium from said reservoir, and d) means for securing said plate to said reservoir so that when said reservoir contains a basal medium, said medium is in contact with the membrane which closes the bottoms of the wells.
Claim 2
Dependent Claim
133 words
A multiple well tissue culture assembly comprising a) a plate comprising a plurality of wells each open at the top and bottom and defining a first predetermined volume, b) a membrane closing off the bottom of each well, said membrane excluding passage therethrough of molecules in excess of a predetermined size between 10,000 and 50,000 daltons, c) a common reservoir defining a second predetermined volume greater than the sum of the first predetermined volumes of a plurality of said wells, said common reservoir having an access port for adding basal medium to and removing basal medium from said reservoir, and d) means for securing said plate to said reservoir so that when said reservoir contains a basal medium, said medium is in contact with the membrane which closes the bottoms of the wells.
Claim 3
Dependent Claim
23 words
An assembly according to claim 1, wherein the membrane closing off the bottom of each well is part of a common semi-permeable membrane.
Claim 4
Dependent Claim
63 words
An assembly according to claim 2, further including a bottom cover for covering the membrane closing off the bottoms of each of said wells, and means for removably connecting said bottom cover to said plate, whereby said plate can be removed from said reservoir and the bottom cover connected thereto for inspection or transport of the contents of the wells of said plate.
Claim 5
Dependent Claim
38 words
An assembly according to claim 1, further comprising at least one additional common reservoir, whereby said plate and membrane closing off the bottoms of each of said wells on said plate can be secured sequentially to either reservoir.
Claim 6
Dependent Claim
63 words
An assembly according to claim 1, further comprising a bottom cover for covering the membrane closing off the bottoms of each of said wells, and means for removably connecting said bottom cover to said plate, whereby said plate can be removed from said reservoir and the bottom cover connected thereto for inspection or transport of the contents of the wells of said plate.
Claim 7
Dependent Claim
56 words
An assembly according to claim 1, wherein said reservoir includes vertical means subdividing it into at least two separate sub-reservoirs, whereby the membrane below some of said wells contacts medium in one of said at least two sub-reservoirs and the membrane below other of said wells contacts medium in another of said at least two sub-reservoirs.
References Cited (Backward Citations)
US2677646A
Cited
US2761813A
Cited
US4012288A
Cited
US4125436A
Cited
US4246339A
Cited
US4308351A
Cited
US4670396A
Cited
US5026649A
Cited
US4797259A
Cited
US4963490A
Cited
US4871674A
Cited
US5139951A
Cited
US5141718A
Cited
US5366893A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-30 | 1999-05-01 | 1999-10-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-30 | 2003-05-01 | 2003-10-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-30 | 2007-05-01 | 2007-10-31 | $7700.00 | Window Closed / Lapsed |