US4797259A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Well-type diagnostic plate device
Assignee: Pall Corp
•
Filing: 1986-12-15
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Granted: 1989-01-10
•
Expires: 2006-12-15
Abstract
Abstract A diagnostic test device is provided including a plate having at least one well, preferably a plurality of wells, each with an open bottom across which a composite membrane comprising three layers is placed, with a hydrophobic, liquid-tight seal provided at the periphery of each well. The composite membrane from the top of upstream side to the bottom or downstream side, in sequence, includes a first reaction or filtration layer formed from a thin, liquophilic, microporous membrane, a second or sealing layer, preferably a hydrophobic material in sheet or fiber form, such as nonwoven polypropylene fibers, and a liquophobic, preferably hydrophobic, barrier layer having one or more apertures which allows liquid to exit the well while eliminating lateral migration of a pendant liquid drop. The liquophobic seal provided by the liquophobic sealing layer eliminates "cross-talk" by lateral diffusion or wicking.
Inventors
- 👤 Vlado I. Matkovich
- 👤 Jerold Martin
- 👤 Peter J. Degen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 04:08
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5025; B01L3/50255; B01D; B01D61/00; B01D61/14; B01D61/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366
Claims Analysis
Total Claims: 39
Claim 1
Independent Claim
92 words
A diagnostic test device comprising: a plate, at least one well formed in said plate, said at least one well having an open bottom, and a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a liquophobic, liquid-tight seal at the periphery of the well.
Claim 2
Dependent Claim
92 words
A diagnostic test device comprising: a plate, at least one well formed in said plate, said at least one well having an open bottom, and a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a liquophobic, liquid-tight seal at the periphery of the well.
Claim 3
Dependent Claim
15 words
The diagnostic test device of claim 1 wherein said reaction layer comprises a hydrophilic material.
Claim 4
Dependent Claim
18 words
The diagnostic test device of claim 1 wherein said sealing layer comprises a synthetic, thermoplastic web of microfibers.
Claim 5
Dependent Claim
17 words
The diagnostic test device of claim 1 wherein said liquophobic, liquid-tight seal is a hydrophobic liquid-tight seal.
Claim 6
Dependent Claim
22 words
The diagnostic test device of claim 1 wherein said reaction layer includes a hole located at substantially the center of said well.
Claim 7
Dependent Claim
18 words
The diagnostic test device of claim 1 wherein said microporous reaction layer comprises a biologically inert polymeric material.
Claim 8
Dependent Claim
20 words
The diagnostic test device of claim 1 wherein said microporous reaction layer is formed from a polyamide or polyvinylidene difluoride.
Claim 9
Dependent Claim
17 words
The diagnostic test device of claim 1 further including a test reagent immobilized on said reaction layer.
Claim 10
Dependent Claim
31 words
The diagnostic test device of claim 1 wherein said barrier layer is formed from a material having a greater liquophobicity than that of either the reaction layer or the sealing layer.
Claim 11
Dependent Claim
17 words
The diagnostic test device of claim 1 wherein said at least one aperture consists of one aperture.
Claim 12
Dependent Claim
18 words
The diagnostic test device of claim 1 wherein said three layers are in intimate contact with one another.
Claim 13
Dependent Claim
16 words
The diagnostic test device of claim 11 wherein said three layers are bonded to one another.
Claim 14
Dependent Claim
18 words
The diagnostic test device of claim 1 wherein said at least one well comprises a plurality of wells.
Claim 15
Dependent Claim
24 words
The diagnostic test device of claim 13 wherein said composite membrane covers and is substantially coextensive with the bottoms of all of said wells.
Claim 16
Dependent Claim
20 words
The diagnostic test device of claim 1 wherein said device is adapted to be operatively associated with a differential pressure.
Claim 17
Dependent Claim
15 words
The diagnostic test device of claim 15 wherein said differential pressure is a reduced pressure.
Claim 18
Dependent Claim
17 words
The diagnostic test device of claim 1 wherein said microporous reaction layer comprises a hydrophilic, surface-modified polyamide.
Claim 19
Dependent Claim
16 words
The diagnostic test device of claim 17 wherein said surface-modified polyamide is a surface-modified nylon 66.
Claim 20
Dependent Claim
15 words
The diagnostic test device of claim 1 wherein said sealing layer comprises a hydrophobic material.
Claim 21
Dependent Claim
35 words
The diagnostic test device of claim 19 wherein said hydrophibic sealing layer is formed from a polyamide, a linear polyester, a polyolefin, a copolymer of two or more olefins, or mixtures of the aforementioned polymers.
Claim 22
Dependent Claim
14 words
The diagnostic test device of claim 20 wherein said hydrophobic sealing layer comprises polypropylene.
Claim 23
Dependent Claim
116 words
A diagnostic test system comprising: (1) a diagnostic test device including: (a) a plate, (b) at least one well formed in said plate, said at least one well having an open bottom, and (c) a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a hydrophobic, liquid-tight seal at the periphery of the well; and (2) means for generating a differential pressure operatively associated with said diagnostic test device.
Claim 24
Dependent Claim
116 words
A diagnostic test system comprising: (1) a diagnostic test device including: (a) a plate, (b) at least one well formed in said plate, said at least one well having an open bottom, and (c) a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a hydrophobic, liquid-tight seal at the periphery of the well; and (2) means for generating a differential pressure operatively associated with said diagnostic test device.
Claim 25
Dependent Claim
17 words
The diagnostic test system of claim 22 wherein said at least one aperture consists of one aperture.
Claim 26
Dependent Claim
30 words
The diagnostic test system of claim 22 wherein said differential pressure generating means comprises a vacuum manifold adapted to be operatively associated with a means for generating a reduced pressure.
Claim 27
Dependent Claim
21 words
The diagnostic test system of claim 24 wherein said vacuum manifold is in air-tight sealing relationship with said diagnostic test device.
Claim 28
Dependent Claim
19 words
The diagnostic test system of claim 24 wherein said vacuum manifold is formed integral with said diagnostic test device.
Claim 29
Dependent Claim
138 words
A diagnostic test device comprising: (a) a first plate, at least one well formed in said first plate, said at least one well having an open bottom, and a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well; and (b) a second plate in liquid receiving relationship with said first plate, at least one well having an impermeable bottom in said second plate to receive liquid from said at least one well in said first plate.
Claim 30
Dependent Claim
138 words
A diagnostic test device comprising: (a) a first plate, at least one well formed in said first plate, said at least one well having an open bottom, and a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well; and (b) a second plate in liquid receiving relationship with said first plate, at least one well having an impermeable bottom in said second plate to receive liquid from said at least one well in said first plate.
Claim 31
Dependent Claim
17 words
The diagnostic test device of claim 27 wherein said at least one aperture consists of one aperture.
Claim 32
Dependent Claim
181 words
A diagnostic test system comprising: (1) a diagnostic test device including: (a) a first plate, (b) at least one well formed in said first plate, said at least one well having an open bottom, and (c) a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well; (2) means for generating a differential pressure operatively associated with said diagnostic test device; and (3) a second plate located intermediate and in operative relationship with said diagnostic test device and said differential pressure generating means, said second plate in liquid receiving relationship with said first plate, at least one well having an impermeable bottom located in said second plate to receive liquid from said at least one well in said first plate.
Claim 33
Dependent Claim
181 words
A diagnostic test system comprising: (1) a diagnostic test device including: (a) a first plate, (b) at least one well formed in said first plate, said at least one well having an open bottom, and (c) a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well; (2) means for generating a differential pressure operatively associated with said diagnostic test device; and (3) a second plate located intermediate and in operative relationship with said diagnostic test device and said differential pressure generating means, said second plate in liquid receiving relationship with said first plate, at least one well having an impermeable bottom located in said second plate to receive liquid from said at least one well in said first plate.
Claim 34
Dependent Claim
17 words
The diagnostic test system of claim 29 wherein said at least one aperture consists of one aperture.
Claim 35
Dependent Claim
30 words
The diagnostic test system of claim 29 wherein said differential pressure generating means comprises a vacuum manifold adapted to be operatively associated with a means for generating a reduced pressure.
Claim 36
Dependent Claim
21 words
The diagnostic test system of claim 31 wherein said vacuum manifold is in air-tight sealing relationship with said diagnostic test device.
Claim 37
Dependent Claim
17 words
The diagnostic test system of claim 31 wherein said second plate is located within said vacuum manifold.
Claim 38
Dependent Claim
19 words
The diagnostic test system of claim 31 wherein said vacuum manifold is formed integral with said diagnostic test device.
Claim 39
Dependent Claim
17 words
The diagnostic test system of claim 34 wherein said second plate is located within said vacuum manifold.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-10 | 1992-07-11 | 1993-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-10 | 1996-07-11 | 1997-01-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-10 | 2000-07-11 | 2001-01-10 | $7700.00 | Window Closed / Lapsed |