US5460959A
Expired / Lapsed
US
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Transduced fibroblasts
Assignee: Biosante Pharmaceuticals Inc
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Filing: 1993-06-01
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Granted: 1995-10-24
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Expires: 2013-06-01
Abstract
Abstract Fibroblasts transduced with genetic material encoding a polypeptide or protein of interest and, optionally, a selectable marker, as well as methods for making and using the transduced fibroblasts. Such fibroblasts are useful in delivering the encoded polypeptide or protein, such as an enzyme, a hormone or a drug, to an individual who has had a graft or implant of the transduced cells.
Inventors
- 👤 Richard C. Mulligan
- 👤 James M. Wilson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:32
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/635; A; A61; A61K; A61K48/00; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/65; C12N15/79; C12N15/85; C12N15/86; A61K38/00; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13041; C12N2740/13043; C12N2740/16011; C12N2740/16041; C12N2740/16043
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
35 words
Fibroblasts transduced by a recombinant retrovirus comprising incorporated genetic material, said transduced fibroblasts bound to a supporting matrix in vitro and having the property of being capable of expressing said incorporated genetic material in vivo.
Claim 2
Dependent Claim
35 words
Fibroblasts transduced by a recombinant retrovirus comprising incorporated genetic material, said transduced fibroblasts bound to a supporting matrix in vitro and having the property of being capable of expressing said incorporated genetic material in vivo.
Claim 3
Dependent Claim
15 words
Fibroblasts according to claim 1, wherein said incorporated genetic material encodes a polypeptide or protein.
Claim 4
Dependent Claim
19 words
Fibroblasts according to claim 1, wherein said incorporated genetic material comprises at least one gene encoding a selectable marker.
Claim 5
Dependent Claim
15 words
Fibroblasts according to claim 3, wherein said gene encoding a selectable marker encodes neomycin resistance.
Claim 6
Dependent Claim
42 words
Fibroblasts according to claim 1, wherein: (a) the genome of said recombinant retrovirus further comprises long terminal repeat sequences, a tRNA binding site, and a Psi packaging site derived from amphotropic Moloney murine leukemia virus; and (b) said recombinant retrovirus is amphotropic.
Claim 7
Dependent Claim
42 words
Fibroblasts according to claim 3, wherein: (a) the genome of said recombinant retrovirus further comprises long terminal repeat sequences, a tRNA binding site, and a Psi packaging site derived from amphotropic Moloney murine leukemia virus; and (b) said recombinant retrovirus is amphotropic.
Claim 8
Dependent Claim
18 words
Fibroblasts according to claim 1, wherein the genome of said recombinant retrovirus further comprises a regulatable eukaryotic promoter.
Claim 9
Dependent Claim
18 words
Fibroblasts according to claim 3, wherein the genome of said recombinant retrovirus further comprises a regulatable eukaryotic promoter.
Claim 10
Dependent Claim
20 words
Fibroblasts according to claim 1, wherein said recombinant retrovirus is selected from the group consisting of pRO, pEM, and pIP.
Claim 11
Dependent Claim
20 words
Fibroblasts according to claim 3, wherein said recombinant retrovirus is selected from the group consisting of pRO, pEM, and pIP.
Claim 12
Dependent Claim
25 words
Fibroblasts according to any one of claims 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, wherein said supporting matrix comprises microcarrier beads.
Claim 13
Independent Claim
50 words
A method of making transduced fibroblasts having the property of being capable of expressing incorporated genetic material in vivo, comprising the steps of: (a) binding fibroblasts to a supporting matrix; and (b) contacting said bound fibroblasts with a recombinant retrovirus comprising incorporated genetic material under conditions appropriate for retroviral infection.
Claim 14
Independent Claim
50 words
A method of making transduced fibroblasts having the property of being capable of expressing incorporated genetic material in vivo, comprising the steps of: (a) binding fibroblasts to a supporting matrix; and (b) contacting said bound fibroblasts with a recombinant retrovirus comprising incorporated genetic material under conditions appropriate for retroviral infection.
Claim 15
Dependent Claim
15 words
The method of claim 12, wherein said incorporated genetic material encodes a polypeptide or protein.
Claim 16
Dependent Claim
19 words
The method of claim 12, wherein said incorporated genetic material comprises at least one gene encoding a selectable marker.
Claim 17
Dependent Claim
17 words
The method of claim 12, further comprising the step of culturing said fibroblasts under suitable growth conditions.
Claim 18
Dependent Claim
17 words
The method of claim 14, further comprising the step of culturing said fibroblasts under suitable growth conditions.
Claim 19
Independent Claim
21 words
The method according to any one of claims 12, 13, 14, 15, or 16, wherein said supporting matrix comprises microcarrier beads.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-23 | 1999-04-24 | 1999-10-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-23 | 2003-04-24 | 2003-10-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-23 | 2007-04-24 | 2007-10-24 | $7700.00 | Window Closed / Lapsed |