Abstract The present invention relates to a method for detecting base sequence differences within homologous regions of two DNA molecules comprising the steps of contacting at least one strand of the first DNA molecule with the complementary strand of the second DNA molecule under conditions such that base pairing occurs, contacting the resulting DNA duplexes with a protein that recognizes substantially all base pair mismatches under conditions such that the protein forms specific complexes with its cognate mispairs, and detecting the resulting DNA:protein complexes by a suitable analytical method. Also disclosed are protein components of DNA mismatch correction systems and the use of these components in methods for genetic mapping.
A method for detecting a base pair mismatch in a DNa duplex, comprising the steps of: a) contacting a DNA duplex potentially containing a base pair mismatch with a protein that recognizes said base pair mismatch under conditions suitable for said protein to form a specific complex only with said DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and b) detecting any said complex as a measure of the presence of a base pair mismatch in said DNa duplex.
Claim 2
Independent Claim
89 words
A method for detecting a base pair mismatch in a DNa duplex, comprising the steps of: a) contacting a DNA duplex potentially containing a base pair mismatch with a protein that recognizes said base pair mismatch under conditions suitable for said protein to form a specific complex only with said DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and b) detecting any said complex as a measure of the presence of a base pair mismatch in said DNa duplex.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein said protein is the product of the mutS gene of Escherichia coli.
Claim 4
Dependent Claim
15 words
The method of claim 2 wherein said protein recognizes all eight possible base pair mismatches.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein said protein is the product of the mutY gene of Escherichia coli.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein said protein is a homolog of the MutS protein of Escherichia coli.
Claim 7
Dependent Claim
18 words
The method of claim 1 wherein said protein is a homolog of the MutY protein of Escherichia coli.