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Patent US5453357A successfully refreshed from Google Patents.
US5453357A
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Pluripotential embryonic stem cells and methods of making same
Assignee: Vanderbilt University
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Filing: 1992-10-08
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Granted: 1995-09-26
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Expires: 2012-10-08
Abstract
Abstract The present invention provides a non-mouse pluripotential embryonic stem cell which can: (a) be maintained on feeder layers for at least 20 passages; and (b) give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Inventors
- 👤 Brigid L. M. Hogan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:42
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; C12N2501/00; C12N2501/10; C12N2501/115; C12N2501/125; C12N2501/20; C12N2501/23; C12N2501/235; C12N2503/00; C12N2503/02; C12N2506/00; C12N2506/04; C12N2510/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
40 words
A composition comprising: (a) pluripotential embryonic stem cells; and (b) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 2
Dependent Claim
40 words
A composition comprising: (a) pluripotential embryonic stem cells; and (b) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 3
Dependent Claim
15 words
The composition of claim 1, wherein the fibroblast growth factor is basic fibroblast growth factor.
Claim 4
Dependent Claim
39 words
A composition comprising: (a) primordial germ cells; and (b) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 5
Dependent Claim
39 words
A composition comprising: (a) primordial germ cells; and (b) a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 6
Dependent Claim
15 words
The composition of claim 3, wherein the fibroblast growth factor is basic fibroblast growth factor.
Claim 7
Dependent Claim
38 words
A composition comprising: (a) embryonic ectoderm cells; and (b) fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 8
Dependent Claim
38 words
A composition comprising: (a) embryonic ectoderm cells; and (b) fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of the cells.
Claim 9
Dependent Claim
34 words
A composition comprising a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of primordial germ cells.
Claim 10
Dependent Claim
34 words
A composition comprising a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow the continued proliferation of primordial germ cells.
Claim 11
Dependent Claim
15 words
The composition of claim 6, wherein the fibroblast growth factor is basic fibroblast growth factor.
Claim 12
Independent Claim
46 words
A method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Claim 13
Independent Claim
46 words
A method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Claim 14
Dependent Claim
14 words
The method of claim 8 wherein the stem cell is derived from a mammal.
Claim 15
Dependent Claim
11 words
The method of claim 9, wherein the mammal is a mouse.
Claim 16
Independent Claim
46 words
A method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to embryonic ectoderm cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Claim 17
Independent Claim
46 words
A method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to embryonic ectoderm cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Claim 18
Dependent Claim
14 words
The method of claim 11, wherein the stem cell is derived from a mammal.
Claim 19
Independent Claim
57 words
A method of screening cells which can be promoted to become pluripotent embryonic stem cells comprising contacting the cells with basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow proliferation of the cells and determining which cells become pluripotent embryonic stem cells.
Claim 20
Independent Claim
57 words
A method of screening cells which can be promoted to become pluripotent embryonic stem cells comprising contacting the cells with basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to enhance the growth of and allow proliferation of the cells and determining which cells become pluripotent embryonic stem cells.
Claim 21
Dependent Claim
33 words
A composition comprising a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to promote the formation of pluripotent embryonic stem cells from primordial germ cells.
Claim 22
Dependent Claim
33 words
A composition comprising a fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor in amounts to promote the formation of pluripotent embryonic stem cells from primordial germ cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1998-09-25 | 1999-03-27 | 1999-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-09-25 | 2003-03-27 | 2003-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-09-25 | 2007-03-27 | 2007-09-26 | $7700.00 | Window Closed / Lapsed |