US5453355A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
Assignee: Abbott Laboratories
•
Filing: 1993-09-03
•
Granted: 1995-09-26
•
Expires: 2013-09-03
Abstract
Abstract The present invention relates to oligonucleotide probes and primers useful in detecting Neisseria gonorrhoeae e.g. by the polymerase chain reaction. The present invention is also directed to methods for detecting Neisseria gonorrhoeae by the polymerase chain reaction. The probes and primers are specific for the pilin gene.
Inventors
- 👤 Larry G. Birkenmeyer
- 👤 Shanfun Ching
- 👤 Yoshihiro Ohhashi
- 👤 Janet K. Winkler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:55
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6876; C12Q1/6888; C12Q1/689; C12Q2600/00; C12Q2600/156
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
24 words
A composition useful in the detection of target DNA from N. gonorrhoeae, said composition comprising primer set 1 (SEQ ID NOS. 2 and 3).
Claim 2
Dependent Claim
24 words
A composition useful in the detection of target DNA from N. gonorrhoeae, said composition comprising primer set 1 (SEQ ID NOS. 2 and 3).
Claim 3
Dependent Claim
118 words
A method for detecting the presence of target DNA from N. gonorrhoeae said method utilizing the polymerase chain reaction and comprising the steps of; a) providing a mixture of a sample suspected of containing said target DNA, an enzyme having DNA polymerase activity, and one or more deoxynucleotide triphosphates; b) denaturing any double stranded DNA in the mixture; c) hybridizing the oligonucleotide primer set of claim 1 to denatured target DNA in said sample; d) extending said primers in a template-dependent manner thereby providing an amplification product; e) repeating steps b) through d) at least once, whereby DNA of N. gonorrhoeae is amplified; and f) detecting said amplification product as a measure of the presence of N. gonorrhoeae.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein said enzyme is heat stable.
Claim 5
Dependent Claim
18 words
The method of claim 3 wherein said heat stable enzyme is derived from a thermophilic Thermus species bacterium.
Claim 6
Dependent Claim
16 words
The method of claim 2 Wherein steps b) through d) are repeated 10 to 50 times.
Claim 7
Dependent Claim
25 words
The method of claim 2 wherein said amplification products are detected by hybridization to an internal probe complementary to one strand of the amplification products.
Claim 8
Dependent Claim
17 words
The method of claim 6 wherein said internal probe has a label capable of generating a signal.
Claim 9
Dependent Claim
40 words
The method of claim 2 wherein at least one primer of said primer pair is bound to a specific binding ligand, and wherein step f) includes capture of said ligand on a solid phase by a receptor for said ligand.
Claim 10
Dependent Claim
44 words
The method of claim 2 wherein at least one primer of said primer pair is bound to a specific binding ligand, and wherein step f) includes attaching a detectable label to said ligand using a receptor for said ligand conjugated to the detectable label.
Claim 11
Dependent Claim
44 words
A kit useful in the specific detection of target DNA from N. gonorrhoeae said kit comprising one or more suitable containers containing: a) primer set 1 (SEQ ID NOS. 2 and 3); b) an enzyme having DNA polymerase activity, and c) four deoxynucleotide triphosphates.
Claim 12
Dependent Claim
44 words
A kit useful in the specific detection of target DNA from N. gonorrhoeae said kit comprising one or more suitable containers containing: a) primer set 1 (SEQ ID NOS. 2 and 3); b) an enzyme having DNA polymerase activity, and c) four deoxynucleotide triphosphates.
Claim 13
Dependent Claim
26 words
The kit according to claim 10 further comprising an internal probe capable of hybridization to the amplification product produced by the primer pair of claim 10.
Claim 14
Dependent Claim
23 words
The kit according to claim 11 wherein said internal probe is an oligonucleotide having 43 bases (SEQ ID NO. 8) as defined herein.
Claim 15
Dependent Claim
15 words
The kit according to claim 10 wherein said enzyme is a heat stable DNA polymerase.
Claim 16
Dependent Claim
20 words
The kit according to claim 13 wherein said heat stable DNA polymerase is derived from a thermophilic Thermus species bacterium.
References Cited (Backward Citations)
US4965188A
Cited
EP0382433A2
Cited
EP0404161A2
Cited
US5162199A
Cited
WO1992013871A1
Cited
WO1993000447A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-09-25 | 1999-03-27 | 1999-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-09-25 | 2003-03-27 | 2003-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-09-25 | 2007-03-27 | 2007-09-26 | $7700.00 | Window Closed / Lapsed |