Patent US5444527A successfully refreshed from Google Patents.
US5444527A
Expired / Lapsed
US
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Imaging flow cytometer for imaging and analyzing particle components in a liquid sample
Assignee: Sysmex Corp
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Filing: 1993-06-02
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Granted: 1995-08-22
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Expires: 2013-06-02
Abstract
Abstract An imaging flow cytometer for capturing images and, analyzing particle components in a liquid sample containing particle components such as blood and urine, or microparticles such as organic high molecules in a suspension. A video camera of a vertical split scanning type is used in the optical system of a conventional particle imaging type flow cytometer. Using the video camera which is capable of scanning a certain limited range in the vertical direction repeatedly in one field period, the number of particles that can be imaged in unit time may be notably increased as compared with the case of using a conventional video camera. By thus constructing, in a flow cytometer possessing a still picture imaging function, the number of captured particles can be increased.
Inventors
- 👤 Tokihiro Kosaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:50
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433; G01N15/1456; G01N15/1459; G01N15/1434; G01N2015/1447
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
132 words
An imaging flow cytometer for passing a sheath blow having a sheath liquid around a sample flow containing particles to be detected, irradiating a sample fine flow with light, and detecting light signals from the particles, comprising: an irradiation light source for illuminating particles in the sample fine flow, imaging means for imaging particles by receiving particle light images in a sample flow region of the sheath flow, and an image processor for processing the images taken by said imaging means, wherein: said imaging means is of the split scanning type capable of repetitively scanning a band-shaped scanning region of an imaging area limited to a certain range of the imaging area within one field period, and said sample flow region is defined within the band-shaped scanning region of the imaging area.
Claim 2
Dependent Claim
132 words
An imaging flow cytometer for passing a sheath blow having a sheath liquid around a sample flow containing particles to be detected, irradiating a sample fine flow with light, and detecting light signals from the particles, comprising: an irradiation light source for illuminating particles in the sample fine flow, imaging means for imaging particles by receiving particle light images in a sample flow region of the sheath flow, and an image processor for processing the images taken by said imaging means, wherein: said imaging means is of the split scanning type capable of repetitively scanning a band-shaped scanning region of an imaging area limited to a certain range of the imaging area within one field period, and said sample flow region is defined within the band-shaped scanning region of the imaging area.
Claim 3
Dependent Claim
38 words
An imaging flow cytometer of claim 1, further comprising: means for detecting incoming of a particle, and wherein light for illuminating a particle is emitted according to particle detection by said means for detecting incoming of a particle.
Claim 4
Dependent Claim
48 words
An imaging flow cytometer of claim 2, further comprising: an image intensifier for intensifying the particle light image being disposed before said imaging means, the image from said image intensifier being taken by said imaging means, wherein the light source for illuminating particles is a white light source.
Claim 5
Dependent Claim
29 words
An imaging flow cytometer of claim 2, further comprising: means for lowering the coherency of the laser light, wherein the light source for illuminating particles is a pulse laser.
Claim 6
Dependent Claim
48 words
An imaging flow cytometer of claim 1, further comprising: an image intensifier for intensifying the particle light image being disposed before said imaging means, the image from said image intensifier being taken by said imaging means, wherein the light source for illuminating particles is a white light source.
Claim 7
Dependent Claim
29 words
An imaging flow cytometer of claim 1 further comprising: means for lowering the coherency of the laser light, wherein the light source for illuminating particles is a pulse laser.
Claim 8
Dependent Claim
26 words
An imaging flow cytometer of claim 1, wherein the flow direction of the sample flow and the longitudinal direction of the band-shaped scanning region are matched.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-08-21 | 1999-02-20 | 1999-08-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-08-21 | 2003-02-20 | 2003-08-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-08-21 | 2007-02-20 | 2007-08-22 | $7700.00 | Window Closed / Lapsed |