Patent US5436330A successfully refreshed from Google Patents.
US5436330A
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US
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Hammerhead ribozymes with enhanced stability provided by an additional 3' ha
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1993-08-24
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Granted: 1995-07-25
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Expires: 2013-08-24
Abstract
Abstract A DNA fragment is disclosed which has connected to the downstream side of a first DNA sequence containing a region coding for the first ribozyme RNA a second DNA sequence containing a region coding for second ribozyme RNA capable of cleaving by self-processing the 3'-terminus site of the first ribozyme RNA subjected to transcription. A ribozyme having the 3'-terminus site thereof self-processed is obtained by effecting transcription of a RNA using as a template a recombinant vector obtained by recombination by the use of the DNA fragment.
Inventors
- 👤 Kazunari Taira
- 👤 Masanao Oda
- 👤 Hideaki Shinshi
- 👤 Kensuke Furukawa
- 👤 Hidekatsu Maeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C07; C07K; C07K2319/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/121; C12N2310/127; C12N2310/50; C12N2310/53
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
6 words
A ribozyme having the formula: ##STR4##
Claim 2
Dependent Claim
6 words
A ribozyme having the formula: ##STR4##
Claim 3
Dependent Claim
118 words
A DNA fragment comprising: (a) a first DNA sequence encoding hammerhead ribozyme RNA, and (b) a second DNA sequence covalently linked to the 3'-terminus of said first DNA sequence, said second DNA sequence encoding (b1) a cleavage site at the 3'-terminus site of RNA transcribed from the said first DNA sequence, (b2) binding site ribozyme RNA which recognizes and binds to said 3'-terminus site of RNA transcribed from said first DNA sequence, and (b3) catalytic hammerhead ribozyme RNA capable of cleaving said transcribed RNA at said 3'-terminus site, wherein said first DNA sequence encodes hammerhead ribozyme RNA which acquires a hairpin configuration due to formation of a nucleotide pair on the 3'-terminus end of said hammerhead ribozyme RNA.
Claim 4
Dependent Claim
118 words
A DNA fragment comprising: (a) a first DNA sequence encoding hammerhead ribozyme RNA, and (b) a second DNA sequence covalently linked to the 3'-terminus of said first DNA sequence, said second DNA sequence encoding (b1) a cleavage site at the 3'-terminus site of RNA transcribed from the said first DNA sequence, (b2) binding site ribozyme RNA which recognizes and binds to said 3'-terminus site of RNA transcribed from said first DNA sequence, and (b3) catalytic hammerhead ribozyme RNA capable of cleaving said transcribed RNA at said 3'-terminus site, wherein said first DNA sequence encodes hammerhead ribozyme RNA which acquires a hairpin configuration due to formation of a nucleotide pair on the 3'-terminus end of said hammerhead ribozyme RNA.
Claim 5
Dependent Claim
19 words
The DNA fragment of claim 2, comprising the following sequence: ##STR5## wherein the symbol ↓ identifies said cleavage site.
Claim 6
Dependent Claim
19 words
The DNA fragment of claim 2, having an RNA polymerase promoter sequence operably covalently linked to the 5'-terminus thereof.
Claim 7
Dependent Claim
10 words
A recombinant vector comprising the DNA fragment of claim 2.
Claim 8
Dependent Claim
18 words
A recombinant vector comprising an RNA polymerase promoter operably covalently linked to the DNA fragment of claim 2.
Claim 9
Dependent Claim
21 words
The recombinant vector of claim 5, wherein said DNA has the sequence: ##STR6## wherein the symbol ↓ identifies said cleavage site.
Claim 10
Dependent Claim
23 words
The recombinant vector of claim 7, further comprising an RNA polymerase promoter sequence operably covalently linked to the 5'-terminus of said DNA sequence.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-07-24 | 1999-01-23 | 1999-07-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-07-24 | 2003-01-23 | 2003-07-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-07-24 | 2007-01-23 | 2007-07-25 | $7700.00 | Window Closed / Lapsed |