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US5434049A
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Separation of polynucleotides using supports having a plurality of electrode-containing cells
Assignee: Hitachi Ltd
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Filing: 1993-02-24
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Granted: 1995-07-18
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Expires: 2013-02-24
Abstract
Abstract A method for simultaneously detecting a plurality of target polynucleotides in a sample on a single reaction chip and a method for separating a plurality of target polynucleotides are provided. On the reaction chip are arranged a plurality of independent cells for capturing different target polynucleotides. Different probes are immobilized onto the individual cells, and labeled probes detect the cells where the target polynucleotides are captured, thereby analyzing the species of the captured target polynucleotides. Also, the individual cells function as electrodes to elute the captured target polynucleotides therethrough, thereby separating a plurality of the target polynucleotides.
Inventors
- 👤 Kazunori Okano
- 👤 Hideki Kambara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:41
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; C12Q1/6837; C12Q1/6816; C12Q1/6825; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00583; B01J2219/00603; B01J2219/00653; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
107 words
A method for separating polynucleotides in a sample solution comprising the steps of; immobilizing individually different probes complementary to a plurality of target polynucleotides onto a plurality of cells formed at different regions on a single polynucleotide capturing support having an electrode on a surface of each of the cells, adding the sample solution to the surface of the support and covering the surface of each cell with the sample solution, for capturing the target polynucleotides onto said polynucleotide capturing support by a hybridization reaction, and supplying an electric field onto the electrode of each cell separately to elute the hybridized target polynucleotides from the cells individually.
Claim 2
Independent Claim
107 words
A method for separating polynucleotides in a sample solution comprising the steps of; immobilizing individually different probes complementary to a plurality of target polynucleotides onto a plurality of cells formed at different regions on a single polynucleotide capturing support having an electrode on a surface of each of the cells, adding the sample solution to the surface of the support and covering the surface of each cell with the sample solution, for capturing the target polynucleotides onto said polynucleotide capturing support by a hybridization reaction, and supplying an electric field onto the electrode of each cell separately to elute the hybridized target polynucleotides from the cells individually.
Claim 3
Dependent Claim
42 words
A method according to claim 1, further comprising a step wherein a capillary provided with another electrode is placed upon each of said cells and the hybridized target polynucleotide on each of said cells is eluted and collected into the capillary individually.
Claim 4
Independent Claim
140 words
A method for separating polynucleotides in a sample solution comprising; immobilizing a plurality of different probes, complementary to a plurality of different target polynucleotides, onto surfaces of a plurality of cells, wherein each cell has only one probe immobilized on a cell surface, each cell is formed at a different region on a single polynucleotide capturing support, and each cell has an electrode on a cell surface, adding the sample solution to the polynucleotide capturing support and covering each cell surface with the sample solution, hybridizing the target polynucleotides to said probes immobilized on the polynucleotide capturing support under hybridization conditions that permit each probe to specifically hybridize to a complementary target polynucleotide, supplying an electric field to each electrode of each cell separately to separately elute the hybridized target polynucleotides from the cells individually, and recovering eluted target polynucleotides.
Claim 5
Independent Claim
140 words
A method for separating polynucleotides in a sample solution comprising; immobilizing a plurality of different probes, complementary to a plurality of different target polynucleotides, onto surfaces of a plurality of cells, wherein each cell has only one probe immobilized on a cell surface, each cell is formed at a different region on a single polynucleotide capturing support, and each cell has an electrode on a cell surface, adding the sample solution to the polynucleotide capturing support and covering each cell surface with the sample solution, hybridizing the target polynucleotides to said probes immobilized on the polynucleotide capturing support under hybridization conditions that permit each probe to specifically hybridize to a complementary target polynucleotide, supplying an electric field to each electrode of each cell separately to separately elute the hybridized target polynucleotides from the cells individually, and recovering eluted target polynucleotides.
Claim 6
Dependent Claim
49 words
A method according to claim 3, further comprising placing a capillary provided with an electrode upon each of said cells before supplying the electric field to each of said cells to elute hybridized target polynucleotide from each of said cells and collecting an eluted target polynucleotide into the capillary.
Claim 7
Dependent Claim
24 words
A method according to claim 3, further comprising washing the cells with an aqueous solution to remove unhybridized polynucleotides before supplying an electric field.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1998-07-17 | 1999-01-16 | 1999-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-07-17 | 2003-01-16 | 2003-07-18 | $3760.00 | Window Closed / Lapsed |
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