Patent US5424207A successfully refreshed from Google Patents.
US5424207A
Expired / Lapsed
US
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Process of revitalizing cells prior to cryopreservation
Assignee: Artivion Inc
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Filing: 1992-08-10
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Granted: 1995-06-13
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Expires: 2012-08-10
Abstract
Abstract A method of revitalizing cells or tissues that are to be cryopreserved for storage at ultracold temperatures, e.g. -196 DEG C. is disclosed which comprises preincubation of the cells or tissue from about 5 minutes to about 24 hours. The preincubation may be conducted at a temperature ranging from about 27 DEG C. to about 42 DEG C., after which the tissue or cells are cryopreserved.
Inventors
- 👤 John F. Carpenter
- 👤 Kelvin G. M. Brockbank
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:04
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/16; A01N1/162
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
89 words
A tissue, comprising one or more cells, that has been cryopreserved and is suitable for transplantation into a human, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium at a temperature of from about 27° C. to about 42° C. and for a time period of at least about 5 minutes, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 2
Dependent Claim
89 words
A tissue, comprising one or more cells, that has been cryopreserved and is suitable for transplantation into a human, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium at a temperature of from about 27° C. to about 42° C. and for a time period of at least about 5 minutes, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 3
Dependent Claim
9 words
A tissue according to claim 1 comprising cardiovascular tissue.
Claim 4
Dependent Claim
18 words
A tissue according to claim 1 wherein the incubation temperature ranges from 35° C. to about 42° C.
Claim 5
Dependent Claim
14 words
A tissue according to claim 1 wherein the incubation temperature is about 37° C.
Claim 6
Dependent Claim
16 words
A tissue according to claim 1 wherein the incubation time is about three to nine hours.
Claim 7
Dependent Claim
13 words
A tissue according to claim 1 wherein the incubation time is six hours.
Claim 8
Dependent Claim
13 words
A tissue according to claim 1 wherein an antibiotic is present during incubation.
Claim 9
Dependent Claim
17 words
A tissue according to claim 1 wherein the tissue has a greater metabolic activity than untreated tissue.
Claim 10
Dependent Claim
26 words
A tissue according to claim 8 wherein the tissue has at least about 1.5 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 11
Dependent Claim
28 words
A tissue according to claim 8 wherein the tissue has at least about 2 to 3 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 12
Dependent Claim
26 words
A tissue according to claim 8 wherein the tissue has at least about 2 times the metabolic activity of untreated tissue as measured by heat dissipation.
Claim 13
Dependent Claim
21 words
A tissue according to claim 1 wherein the incubation time is in the range of about 5 minutes to 24 hours.
Claim 14
Dependent Claim
97 words
A transplantable tissue, comprising one or more cells, that has been cryopreserved and that has been subjected to periods of warm and/or cold ischemia prior to cryopreservation, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium at a temperature of from about 27° C. to about 42° C. and for a time period of at least about 5 minutes, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 15
Dependent Claim
97 words
A transplantable tissue, comprising one or more cells, that has been cryopreserved and that has been subjected to periods of warm and/or cold ischemia prior to cryopreservation, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium at a temperature of from about 27° C. to about 42° C. and for a time period of at least about 5 minutes, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 16
Dependent Claim
21 words
A tissue according to claim 13 wherein the incubation time is in the range of about 5 minutes to 24 hours.
Claim 17
Dependent Claim
9 words
A tissue according to claim 13 comprising cardiovascular tissue.
Claim 18
Dependent Claim
18 words
A tissue according to claim 13 wherein the incubation temperature ranges from 35° C. to about 42° C.
Claim 19
Dependent Claim
14 words
A tissue according to claim 13 wherein the incubation temperature is about 37° C.
Claim 20
Dependent Claim
16 words
A tissue according to claim 13 wherein the incubation time is about three to nine hours.
Claim 21
Dependent Claim
13 words
A tissue according to claim 13 wherein the incubation time is six hours.
Claim 22
Dependent Claim
14 words
A tissue according to claim 13 wherein an antibiotic is present during the incubation.
Claim 23
Dependent Claim
17 words
A tissue according to claim 13 wherein the tissue has a greater metabolic activity than untreated tissue.
Claim 24
Dependent Claim
26 words
A tissue according to claim 21 wherein the tissue has at least about 2 times the metabolic activity of untreated tissue as measured by heat dissipation.
Claim 25
Dependent Claim
26 words
A tissue according to claim 21 wherein the tissue has at least about 1.5 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 26
Dependent Claim
28 words
A tissue according to claim 21 wherein the tissue has at least about 2 to 3 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 27
Dependent Claim
18 words
A tissue according to claim 13 wherein the periods of warm and cold ischemia occur prior to incubation.
Claim 28
Dependent Claim
90 words
A cardiovascular tissue, comprising one or more cells, that has been cryopresserved and is suitable for transplantation into a human, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium and in the presence of an antibiotic at a temperature of from about 27° C. to about 42° C. for about six hours, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 29
Dependent Claim
90 words
A cardiovascular tissue, comprising one or more cells, that has been cryopresserved and is suitable for transplantation into a human, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium and in the presence of an antibiotic at a temperature of from about 27° C. to about 42° C. for about six hours, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 30
Dependent Claim
10 words
A tissue according to claim 26 comprising a heart valve.
Claim 31
Dependent Claim
26 words
A tissue according to claim 26 wherein the tissue has at least about 2 times the metabolic activity of untreated tissue as measured by heat dissipation.
Claim 32
Dependent Claim
26 words
A tissue according to claim 26 wherein the tissue has at least about 1.5 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 33
Dependent Claim
28 words
A tissue according to claim 26 wherein the tissue has at least about 2 to 3 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 34
Dependent Claim
104 words
A cardiovascular tissue, comprising one or more cells and suitable for transplantation into a human, that has been cryopreserved and that has been subjected to periods of warm and/or cold ischemia prior to cryoperservation, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium and in the presence of an antibiotic at a temperature of from about 27° C. to about 42° C. for about six hours, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 35
Dependent Claim
104 words
A cardiovascular tissue, comprising one or more cells and suitable for transplantation into a human, that has been cryopreserved and that has been subjected to periods of warm and/or cold ischemia prior to cryoperservation, said tissue having been incubated prior to freezing and prior to the addition of a cryoprotectant in a nutrient medium and in the presence of an antibiotic at a temperature of from about 27° C. to about 42° C. for about six hours, such conditions being adapted to provide a tissue which, upon thawing and use, has improved viability and/or functional capacity over tissue not subjected to such an incubation.
Claim 36
Dependent Claim
18 words
A tissue according to claim 31 wherein the periods of warm and cold ischemia occur prior to incubation.
Claim 37
Dependent Claim
10 words
A tissue according to claim 31 comprising a heart valve.
Claim 38
Dependent Claim
26 words
A tissue according to claim 31 wherein the tissue has at least about 2 times the metabolic activity of untreated tissue as measured by heat dissipation.
Claim 39
Dependent Claim
26 words
A tissue according to claim 31 wherein the tissue has at least about 1.5 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
Claim 40
Dependent Claim
28 words
A tissue according to claim 31 wherein the tissue has at least about 2 to 3 times the metabolic activity of untreated tissue as measured by 2-deoxyglucose incorporation.
References Cited (Backward Citations)
US3545221A
Cited
US3810367A
Cited
US4559298A
Cited
FR2589165A1
Cited
WO1988002832A1
Cited
EP0296475A1
Cited
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