US5397479A
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US
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Composition and method for enrichment of white blood cells from whole human blood
Assignee: Iris International Inc
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Filing: 1993-04-26
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Granted: 1995-03-14
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Expires: 2013-04-26
Abstract
Abstract A composition and method for separating red blood cells from whole blood including a rouleaux-forming aggregator and an enhancer for enhancing the settling rate. The enhancer is a material which alters directly or indirectly the properties of the red blood cell and may alter the structure and/or reactivity of the aggregator, without adversely affecting the morphology and function of white blood cells. In most instances, the red blood cell enhancers of the invention are osmotic agents. Such agents create a hypertonic solution while not entering the cells themselves. Preferably the enhancer is a salt of oxalic acid, a salt of malonic acid, mannitol or sucrose. Potassium oxalate is most preferred. High molecular weight substances which are large enough to form molecular bridges between red blood cells form the aggregators used in the invention. Examples of useful aggregators include dextran, hespan, pentaspan, ficoll, gum arabic, polyvinylpyrrolidone, nucleic acids, and proteins such as fibrinogen and gamma globulins.
Inventors
- 👤 Lawrence Kass
- 👤 Leonard Spolter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 16:09
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/18; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
57 words
A method for separating blood cells from a solution containing said cells comprising the steps of mixing a liquid sample of whole blood with a settling solution, where said settling solution comprises dextran having a molecular weight greater than about 40,000 and a member selected from the group consisting of potassium oxalate, potassium malonate, mannitol and sucrose.
Claim 2
Independent Claim
57 words
A method for separating blood cells from a solution containing said cells comprising the steps of mixing a liquid sample of whole blood with a settling solution, where said settling solution comprises dextran having a molecular weight greater than about 40,000 and a member selected from the group consisting of potassium oxalate, potassium malonate, mannitol and sucrose.
Claim 3
Independent Claim
48 words
A method for separating red blood cells comprising the steps of mixing a liquid sample of whole blood with a settling solution, where said solution comprises a rouleaux forming aggregator selected from the group consisting of synthetic polymers, polysaccharides, proteins, and nucleic acids and a settling rate enhancer.
Claim 4
Independent Claim
48 words
A method for separating red blood cells comprising the steps of mixing a liquid sample of whole blood with a settling solution, where said solution comprises a rouleaux forming aggregator selected from the group consisting of synthetic polymers, polysaccharides, proteins, and nucleic acids and a settling rate enhancer.
Claim 5
Dependent Claim
29 words
A method as in claim 2 wherein said rouleaux forming aggregator is selected from the group consisting of dextran, hespan, pentaspan, ficoll, gum arabic, polyvinylpyrrolidone, gamma globulin, and fibrinogen.
Claim 6
Dependent Claim
22 words
A method as in claim 1 wherein said rouleaux forming aggregator is dextran having a molecular weight of about 70,000 or greater.
Claim 7
Dependent Claim
27 words
A method as in claim 2 wherein said enhancer is selected from the group consisting of salts of oxalic acid, salts of malonic acid, mannitol and sucrose.
Claim 8
Dependent Claim
12 words
A method as in claim 1 wherein said member is potassium malonate.
Claim 9
Dependent Claim
21 words
A method as in claim 2 wherein said aggregator is polyvinylpyrrolidone, gum arabic or ficoll and said enhancer is potassium oxalate.
Claim 10
Dependent Claim
12 words
A method as in claim 1 wherein said member is potassium oxalate.
Claim 11
Independent Claim
73 words
A method for separating red blood cells from whole blood comprising the steps of combining a rouleaux forming aggregator selected from the group consisting of dextran having a molecular weight greater than about 40,000, gum arabic, ficoll, and polyvinylpyrrolidone, a solution containing an enhancer for increasing the settling rate of said cells selected from the group consisting of salts of malonate acid, and salts of oxalic acid, mannitol and sucrose, and whole blood.
Claim 12
Independent Claim
73 words
A method for separating red blood cells from whole blood comprising the steps of combining a rouleaux forming aggregator selected from the group consisting of dextran having a molecular weight greater than about 40,000, gum arabic, ficoll, and polyvinylpyrrolidone, a solution containing an enhancer for increasing the settling rate of said cells selected from the group consisting of salts of malonate acid, and salts of oxalic acid, mannitol and sucrose, and whole blood.
Claim 13
Dependent Claim
18 words
A method as in claim 9 wherein said aggregator is dextran having a molecular weight of about 500,000.
Claim 14
Dependent Claim
11 words
A method as in claim 9 wherein said enhancer comprises mannitol.
Claim 15
Dependent Claim
11 words
A method as in claim 9 wherein said enhancer comprises sucrose.
Claim 16
Dependent Claim
15 words
A method as in claim 9 wherein said enhancer comprises a salt of oxalic acid.
Claim 17
Dependent Claim
15 words
A method as in claim 9 wherein said enhancer comprises a salt of malonic acid.
Claim 18
Dependent Claim
12 words
A method as in claim 13 wherein said enhancer is potassium oxalate.
Claim 19
Dependent Claim
12 words
A method as in claim 14 wherein said enhancer is potassium malonate.
Claim 20
Dependent Claim
11 words
A method as in claim 10 wherein said enhancer comprises mannitol.
Claim 21
Dependent Claim
11 words
A method as in claim 10 wherein said enhancer comprises sucrose.
Claim 22
Dependent Claim
15 words
A method as in claim 10 wherein said enhancer comprises a salt of oxalic acid.
Claim 23
Dependent Claim
15 words
A method as in claim 10 wherein said enhancer comprises a salt of malonic acid.
Claim 24
Dependent Claim
12 words
A method as in claim 19 wherein said enhancer is potassium oxalate.
Claim 25
Dependent Claim
12 words
A method as in claim 20 wherein said enhancer is potassium malonate.
Claim 26
Independent Claim
65 words
A method for separating red blood cells comprising the steps of mixing a liquid sample containing red blood cells with a settling solution, where said solution comprises a first component selected from the group consisting of hespan, pentaspan, gamma globulin and fibrinogen and a second component selected from the group consisting of a salt of malonic acid, a salt of oxalic acid, mannitol and sucrose.
Claim 27
Independent Claim
65 words
A method for separating red blood cells comprising the steps of mixing a liquid sample containing red blood cells with a settling solution, where said solution comprises a first component selected from the group consisting of hespan, pentaspan, gamma globulin and fibrinogen and a second component selected from the group consisting of a salt of malonic acid, a salt of oxalic acid, mannitol and sucrose.
Claim 28
Dependent Claim
12 words
The method of claim 23 wherein said second component is potassium malonate.
Claim 29
Dependent Claim
12 words
The method of claim 24 wherein said second component is potassium oxalate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-03-13 | 1998-09-12 | 1999-03-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-03-13 | 2002-09-12 | 2003-03-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-03-13 | 2006-09-12 | 2007-03-14 | $7700.00 | Window Closed / Lapsed |