US5118428A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method to remove red blood cells from whole blood samples
Assignee: Individual
•
Filing: 1990-11-13
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Granted: 1992-06-02
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Expires: 2010-11-13
Abstract
Abstract Red Blood Cells (RBC's) are removed from whole blood samples by contacting the whole blood sample with a solution containing an acid selected from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid, and malonic acid. The agglutinated RBC's are then removed from the resultant suspension by procedures of filtration, centrifugation or decantation, leaving an essentially RBC-free serum or plasma sample.
Inventors
- 👤 Theodore T. Sand
- 👤 Barbara McLaughlin
- 👤 William P. Kolb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 19:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
97 words
A process for removing Red Blood Cells (RBC's) from whole blood comprising: a) contacting a solution containing an acid from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid and malonic acid, with a whole blood sample, the acid being present in a concentration and in an amount and at a pH effective to agglutinate the RBC's in the whole blood and obtain a resultant suspension; and b) removing the agglutinated RBC's from the resultant suspension by filtering the suspension through a fibrous material to produce an essentially RBC-free sample.
Claim 2
Dependent Claim
97 words
A process for removing Red Blood Cells (RBC's) from whole blood comprising: a) contacting a solution containing an acid from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid and malonic acid, with a whole blood sample, the acid being present in a concentration and in an amount and at a pH effective to agglutinate the RBC's in the whole blood and obtain a resultant suspension; and b) removing the agglutinated RBC's from the resultant suspension by filtering the suspension through a fibrous material to produce an essentially RBC-free sample.
Claim 3
Dependent Claim
25 words
The process of claim 1 wherein the fibrous material is selected from the group consisting of glass wool, cotton, glass fiber or a combination thereof.
Claim 4
Dependent Claim
29 words
The process of claim 1 wherein the acid is acetic acid and the pH is of a value that maintains the acetic acid in predominantly the free acid form.
Claim 5
Dependent Claim
29 words
The process of claim 1 wherein the acid is citric acid and the pH is of a value that maintains the citric acid in predominantly the free acid form.
Claim 6
Dependent Claim
28 words
The process of claim 1 wherein the acid is ascorbic acid and the pH is of a value that maintains the ascorbic acid in predominantly the salt form.
Claim 7
Dependent Claim
28 words
The process of claim 1 wherein the acid is lactic acid and the pH is of a value that maintains the lactic acid in predominantly the salt form.
Claim 8
Dependent Claim
28 words
The process of claim 1 wherein the acid is maleic acid and the pH is of a value that maintains the maleic acid in predominantly the salt form.
Claim 9
Dependent Claim
28 words
The process of claim 1 wherein the acid is malic acid and the pH is of a value that maintains the malic acid in predominantly the salt form.
Claim 10
Dependent Claim
28 words
The process of claim 1 wherein the acid is malonic acid and the pH is of a value that maintains the malonic acid in predominantly the salt form.
Claim 11
Dependent Claim
118 words
A process for removing RBC's from whole blood comprising: a) contacting a solution containing an acid selected from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid and malonic acid, with a whole blood sample, the acid being present in a concentration and in an amount and at a pH effective to agglutinate the RBC's in the whole blood and obtain a resultant suspension; and b) removing the agglutinated RBC's from the resultant suspension to produce an essentially RBC-free sample; wherein the removing is by filtering and wherein the filtering is carried out in a dispensetube tip containing glass fiber and cotton that dispenses the RBC-free sample onto a test device.
Claim 12
Dependent Claim
118 words
A process for removing RBC's from whole blood comprising: a) contacting a solution containing an acid selected from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid and malonic acid, with a whole blood sample, the acid being present in a concentration and in an amount and at a pH effective to agglutinate the RBC's in the whole blood and obtain a resultant suspension; and b) removing the agglutinated RBC's from the resultant suspension to produce an essentially RBC-free sample; wherein the removing is by filtering and wherein the filtering is carried out in a dispensetube tip containing glass fiber and cotton that dispenses the RBC-free sample onto a test device.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-06-02 | 1995-12-02 | 1996-06-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-06-02 | 1999-12-02 | 2000-06-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-06-02 | 2003-12-02 | 2004-06-02 | $7700.00 | Window Closed / Lapsed |