EP0287731A2
Expired / Lapsed
EP
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Dry test strips having a red blood cell exclusion layer
Assignee: Genesis Labs Inc
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Filing: 1987-04-30
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Granted: —
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Expires: 2007-04-30
Abstract
Abstract A dry test strip, for the detection of analytes in liquids containing red blood cells, typically whole blood, comprises a detection zone containing reactant system that can generate a unique signal in the presence of an analyte wherein RBC in the test fluid are prevented from entering the detection zone by a barrier comprising a polysaccharide material. The unique signal generated by the reagent system in the detection zone is visualized through the carrier strip opposite the barrier layer.
Inventors
- 👤 Catherine B. Maddox
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 16:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/60
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
84 words
A dry test strip for the detection of an analyte, in a test fluid containing red blood cells, said detection occurs in the absence of interference between signal generation and the presence of the red blood cells, which dry test strip comprises (a) a signal generating zone containing a signal generating system that produces a unique signal in the presence of the analyte, and (b) an effective concentration of a polysaccharide material sufficient to prevent penetration of red blood cells through the detection zone.
Claim 2
Dependent Claim
84 words
A dry test strip for the detection of an analyte, in a test fluid containing red blood cells, said detection occurs in the absence of interference between signal generation and the presence of the red blood cells, which dry test strip comprises (a) a signal generating zone containing a signal generating system that produces a unique signal in the presence of the analyte, and (b) an effective concentration of a polysaccharide material sufficient to prevent penetration of red blood cells through the detection zone.
Claim 3
Dependent Claim
15 words
A dry test strip as claimed in claim 1 wherein the polysaccharide material comprises dextran.
Claim 4
Dependent Claim
27 words
A dry test strip as claimed in claim 2 wherein the dextran has a molecular weight of about 10,000-50,000 and is dispersed uniformly throughout the detection zone.
Claim 5
Dependent Claim
24 words
A dry test strip as claimed in claim 2 or 3 wherein the dextran comprises a polymer of D-glucose having (1 → 6) linkages.
Claim 6
Dependent Claim
28 words
A dry test strip as claimed in any of claims 1 to 4 wherein the detection system in the detection zone comprises a chemical assay or an immunoassay.
Claim 7
Dependent Claim
23 words
A dry test strip as claimed in claim 5 wherein the chemical assay comprises an assay for glucose or an assay for cholesterol.
Claim 8
Dependent Claim
51 words
A dry test strip as claimed in claim 5 wherein the detection zone comprises a matrix of a first labeled reagent zone containing a labeled antibody or fragment thereof, a second trapping zone comprising immobilized antigen, and a third signal generation zone containing means to detect the presence of the label.
Claim 9
Dependent Claim
44 words
A dry test strip as claimed in any of claims 1 to 7 wherein the test fluid comprises a fluid, containing significant numbers of red blood cells, selected from the group consisting of serum, plasma, urine, cerebral spinal fluid, ascities, saliva, or mixtures thereof.
Claim 10
Dependent Claim
32 words
A dry test strip as claimed in any of claims 1 to 7 wherein the test fluid comprises a whole blood, a fraction thereof or a suspension thereof in a compatible diluent.
Claim 11
Dependent Claim
27 words
A dry test strip as claimed in any of claims 1 to 9 wherein the dry test strip additionally comprises a carrier strip comprising a polymeric film.
Claim 12
Dependent Claim
89 words
A dry test strip for detection of an analyte, in a test fluid containing red blood cells, in the absence of interference between red blood cells and signal generation which comprises a carrier strip having a signal generating zone containing a signal generating system that produces a unique signal in the presence of an analyte, said signal generating zone having in a discrete barrier layer on the surface of the zone, a sufficient amount of dextran to prevent the penetration of the signal generating zone by red blood cells.
Claim 13
Dependent Claim
89 words
A dry test strip for detection of an analyte, in a test fluid containing red blood cells, in the absence of interference between red blood cells and signal generation which comprises a carrier strip having a signal generating zone containing a signal generating system that produces a unique signal in the presence of an analyte, said signal generating zone having in a discrete barrier layer on the surface of the zone, a sufficient amount of dextran to prevent the penetration of the signal generating zone by red blood cells.
Claim 14
Dependent Claim
19 words
A dry test strip as claimed in claim 11 wherein the dextran has a molecular weight of about 10,000-50,000.
Claim 15
Dependent Claim
24 words
A dry test strip as claimed in claim 11 or 12 wherein the dextran comprises a polymer of D-glucose having (1 → 6) linkages.
Claim 16
Dependent Claim
29 words
A dry test strip as claimed in any of claims 11 to 13 wherein the signal generating system in the detection zone comprises a chemical assay or an immunoassay.
Claim 17
Dependent Claim
55 words
A dry test strip as claimed in any of claims 11 to 14 wherein the detection zone comprises a matrix of a first labeled reagent zone containing a labeled antibody or fragment thereof, a second trapping zone comprises immobilized antigen and a third single generation zone containing means to detect the presence of the label.
Claim 18
Dependent Claim
44 words
A dry test strip as claimed in any of claims 11 to 15 wherein the test fluid comprises a fluid containing significant numbers of red blood cells selected from the group consisting of serum, plasma, urine, cerebral spinal fluid, ascites, saliva, or mixtures thereof.
Claim 19
Dependent Claim
31 words
A dry test strip as claimed in any of claims 11 to 15 wherein the test fluid comprises whole blood, a fraction thereof or a suspension thereof in a compatible diluent.
Claim 20
Independent Claim
70 words
A method for detecting an analyte in a test fluid containing red blood cells, said detection occurring in the absence of interference between signal generation and the presence of red blood cells, which method comprises applying a test fluid containing red blood cells to a dry test strip as claimed in any of claims 1 to 17, and detecting the concentration of the analyte through the degree of signal produced.
References Cited (Backward Citations)
No citation records indexed.
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