US5378467A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell-free Marek's disease virus vaccine
Assignee: Intervet International BV
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Filing: 1993-11-23
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Granted: 1995-01-03
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Expires: 2013-11-23
Abstract
Abstract The present invention is concerned with a vaccine for the protection of poultry against Marek's Disease. The cell free vaccine of the invention facilitates the handling of the vaccine and reduces the chance of physical abuse. The invention also relates to bivalent or polyvalent vaccines comprising in addition other viruses of the Marek's Disease virus group, i.e. HVT.
Inventors
- 👤 William Bexendale
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:20
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K39/00; A61K39/12; A61K39/245; A61K39/255; A61P; A61P31/00; A61P31/12; A61P31/20; A61P31/22; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16311; C12N2710/16334; C12N2710/16361; C12N2710/16364
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
22 words
A vaccine for the protection of poultry against Marek's Disease, comprising cell-free Marek's Disease serotype 2 virus, and a pharmaceutically acceptable carrier.
Claim 2
Dependent Claim
22 words
A vaccine for the protection of poultry against Marek's Disease, comprising cell-free Marek's Disease serotype 2 virus, and a pharmaceutically acceptable carrier.
Claim 3
Dependent Claim
14 words
A vaccine according to claim 1, wherein the virus is a cell-free SB-1 virus.
Claim 4
Dependent Claim
23 words
A vaccine according to claim 1, wherein the dosage of the cell-free Marek's Disease serotype 2 virus is at least 2.2 log pfu/chick.
Claim 5
Dependent Claim
15 words
A vaccine according to claim 3, wherein said dosage is at least 2.7 log pfu/chick.
Claim 6
Dependent Claim
15 words
A vaccine according to claim 4, wherein said dosage is at least 3.2 log pfu/chick.
Claim 7
Dependent Claim
10 words
A vaccine according to claim 1, additionally comprising cell-free HVT.
Claim 8
Dependent Claim
11 words
A vaccine according to claim 1, wherein said vaccine is lyophilized.
Claim 9
Independent Claim
71 words
A method for the preparation of a vaccine that protects poultry against Marek's Disease comprising: a) growing a serotype 2 Marek's Disease virus in a cell culture from which sufficient quantities of cell-free virus necessary to prepare an effective immunizing dosage can be obtained, b) disrupting the cells, and c) collecting the cell-free viruses, and mixing them with cell-free HVT, such as to prepare an effective immunizing dosage of said vaccine.
Claim 10
Independent Claim
71 words
A method for the preparation of a vaccine that protects poultry against Marek's Disease comprising: a) growing a serotype 2 Marek's Disease virus in a cell culture from which sufficient quantities of cell-free virus necessary to prepare an effective immunizing dosage can be obtained, b) disrupting the cells, and c) collecting the cell-free viruses, and mixing them with cell-free HVT, such as to prepare an effective immunizing dosage of said vaccine.
Claim 11
Dependent Claim
21 words
A method according to claim 8, wherein the titre of cell-free virus after step b is at least 4 logs pfu/ml.
Claim 12
Dependent Claim
23 words
A method of controlling Marek's Disease in poultry comprising administering an immunologically effective amount of the vaccine according to claim 1 to poultry.
Claim 13
Dependent Claim
18 words
A method according to claim 8, wherein additionally the collected cell-free viruses are clarified by centrifugation or filtration.
Claim 14
Dependent Claim
14 words
A method according to claim 8, wherein additionally the collected cell-free viruses are freeze-dried.
Claim 15
Dependent Claim
16 words
A method according to claim 8, wherein additionally a buffer is added to the cell-free viruses.
Claim 16
Dependent Claim
17 words
A method according to claim 8, wherein additionally a stabilizing agent is added to the cell-free viruses.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-02 | 1998-07-04 | 1999-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-02 | 2002-07-04 | 2003-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-02 | 2006-07-04 | 2007-01-03 | $7700.00 | Window Closed / Lapsed |