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US5371089A
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Method and composition for ameliorating the adverse effects of aging
Assignee: Senetek PLC
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Filing: 1994-03-04
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Granted: 1994-12-06
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Expires: 2014-03-04
Abstract
Abstract Compositions and methods are provided for countering the adverse effects of aging on cells in culture and in vivo in which cells are contacted with the compositions that ameliorate the adverse effects of aging on mammalian cells by slowing or reversing the changes that normally accompanying aging of such cells but do not significantly increase the growth rate or total proliferative capacity of such cells. The compositions contain one or more 6-(substituted amino)purine cytokinins and preferably do not contain ingredients that promote cell division or that induce or potentiate the ability of the 6-(substituted amino) purine cytokinins to promote cell division. Among the preferred applications of the compositions and methods provided herein are the preservation of or restoration of the health of mammalian cells in culture and, by application of the compositions to human skin, the health and youthful appearance of the skin.
Inventors
- 👤 Suresh I. S. Rattan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 15:39
CPC Classifications
A; A61; A61Q; A61Q19/00; A61Q19/08; A61K; A61K31/00; A61K31/33; A61K31/395; A61K31/495; A61K31/505; A61K31/519; A61K31/52; A61K8/00; A61K8/18; A61K8/30; A61K8/49; A61K8/60; A61K8/606
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
63 words
A method for treating mammalian skin in order to improve its cosmetic appearance, said method comprising topically applying to said mammalian skin an effective amount of one or more 6-(substituted amino)purine cytokinins, wherein the amount applied is sufficient to improve the cosmetic appearance of said mammalian skin but does not alter the growth rate and total proliferative capacity of the mammalian skin cells.
Claim 2
Independent Claim
63 words
A method for treating mammalian skin in order to improve its cosmetic appearance, said method comprising topically applying to said mammalian skin an effective amount of one or more 6-(substituted amino)purine cytokinins, wherein the amount applied is sufficient to improve the cosmetic appearance of said mammalian skin but does not alter the growth rate and total proliferative capacity of the mammalian skin cells.
Claim 3
Dependent Claim
63 words
The method of claim 1, wherein the improvement in cosmetic appearance is manifested by a reduction in the number or depth of wrinkles in said mammalian skin or by a delay in the development of wrinkles in said mammalian skin and wherein said 6-(substituted amino)purine cytokinin is contained in a lotion, ointment, cream, or gel suitable for topical application to said mammalian skin.
Claim 4
Dependent Claim
15 words
The method of claim 1, wherein the mammalian skin to be treated is human skin.
Claim 5
Dependent Claim
15 words
The method of claim 2, wherein the mammalian skin to be treated is human skin.
Claim 6
Dependent Claim
50 words
The method of claim 3, wherein the 6-(substituted amino)purine cytokinins are of general formula ##STR5## wherein R 1 is a radical selected from the group consisting of furfuryl radical, phenyl radical, benzyl radical, n-alkyl and branched alkyl radicals containing 4, 5, or 6 carbon atoms, (cyclohexyl)methyl radical, and 3-hydroxymethyl-3-methylallyl radical.
Claim 7
Dependent Claim
51 words
The method of claim 4, wherein the 6-(substituted amino)purine cytokinins are of general formula ##STR6## wherein R 1 is a radical selected from the group consisting of furfuryl radical, phenyl radical, benzyl radical, n-alkyl and branched alkyl radicals containing 4, 5, or 6 carbon atoms, (cyclohexyl) methyl radical, and 3-hydroxymethyl-3-methylallyl radical.
Claim 8
Dependent Claim
12 words
The method of claim 5, wherein R 1 is the furfuryl radical.
Claim 9
Dependent Claim
12 words
The method of claim 6, wherein R 1 is the furfuryl radical.
Claim 10
Dependent Claim
12 words
The method of claim 5, wherein R 1 is the 3-hydroxymethyl-3-methylallyl radical.
Claim 11
Dependent Claim
12 words
The method of claim 6, wherein R 1 is the 3-hydroxymethyl-3-methylallyl radical.
Claim 12
Dependent Claim
31 words
The method of claim 5, wherein the amount of 6-(substituted amino)purine cytokinins in the lotion, ointment, cream, or gel is in the range of about 50 ppm to about 1,000 ppm.
Claim 13
Dependent Claim
31 words
The method of claim 6, wherein the amount of 6-(substituted amino)purine cytokinins in the lotion, ointment, cream, or gel is in the range of about 50 ppm to about 1,000 ppm.
Claim 14
Independent Claim
101 words
A method for treating human skin in order to improve its cosmetic appearance, said method comprising topically applying to said human skin a composition containing an amount of one or more 6-(substituted amino)purine cytokinins sufficient to improve the cosmetic appearance of said human skin, wherein the 6-(substituted amino)purine cytokinins do not significantly alter the growth rate and total proliferative capacity of the human skin cells and wherein the improvement in cosmetic appearance is manifested by a reduction in the number or depth of wrinkles in said human skin or by a delay in the development of wrinkles in said human skin.
Claim 15
Independent Claim
101 words
A method for treating human skin in order to improve its cosmetic appearance, said method comprising topically applying to said human skin a composition containing an amount of one or more 6-(substituted amino)purine cytokinins sufficient to improve the cosmetic appearance of said human skin, wherein the 6-(substituted amino)purine cytokinins do not significantly alter the growth rate and total proliferative capacity of the human skin cells and wherein the improvement in cosmetic appearance is manifested by a reduction in the number or depth of wrinkles in said human skin or by a delay in the development of wrinkles in said human skin.
Claim 16
Dependent Claim
19 words
The method of claim 13, wherein the composition is a lotion, ointment, cream, or gel suitable for topical application.
Claim 17
Dependent Claim
50 words
The method of claim 13, wherein the 6-(substituted amino)purine cytokinins are of general formula ##STR7## wherein R 1 is a radical selected from the group consisting of furfuryl radical, phenyl radical, benzyl radical, n-alkyl and branched alkyl radicals containing 4, 5, or 6 carbon atoms, (cyclohexyl)methyl radical, and 3-hydroxymethyl-3-methylallyl radical.
Claim 18
Dependent Claim
19 words
The method of claim 15, wherein the composition is a lotion, ointment, cream, or gel suitable for topical application.
Claim 19
Dependent Claim
12 words
The method of claim 15, wherein R 1 is the furfuryl radical.
Claim 20
Dependent Claim
12 words
The method of claim 15, wherein R 1 is the 3-hydroxymethyl-3-methylallyl radical.
Claim 21
Dependent Claim
27 words
The method of claim 15, wherein the amount of 6-(substituted amino)purine cytokinins in the composition is in the range of about 10 ppm and about 5,000 ppm.
Claim 22
Dependent Claim
27 words
The method of claim 15, wherein the amount of 6-(substituted amino)purine cytokinins in the composition is in the range of about 100 ppm and about 1,000 ppm.
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