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US5369003A
Expired / Lapsed
US
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Process for the specific production of ribonucleic acids
Assignee: Roche Diagnostics GmbH
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Filing: 1992-09-28
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Granted: 1994-11-29
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Expires: 2012-09-28
Abstract
Abstract Process for the production of ribonucleic acids or for the detection of template nucleic acids based on a transcription technique using two oligonucleotides which can hybridize to adjacent regions on the template nucleic acid without ligation of these oligonucleotides.
Inventors
- 👤 Udo Reischl
- 👤 Rudiger Rueger
- 👤 Christoph Kessler
- 👤 Rudolf Seibl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6865
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
77 words
A process for the specific production of ribonucleic acids comprising the steps of: a) hybridizing a template specific promotor oligonucleotide P1 and a template specific oligonucleotide P2 with a template nucleic acid T to form a complex K, and b) forming transcripts R containing at least the template specific sequence information from P1 to P2 by promotor controlled transcription, wherein the oligonucleotides P1 and P2 used are not enzymatically ligated together in any step in the process.
Claim 2
Dependent Claim
77 words
A process for the specific production of ribonucleic acids comprising the steps of: a) hybridizing a template specific promotor oligonucleotide P1 and a template specific oligonucleotide P2 with a template nucleic acid T to form a complex K, and b) forming transcripts R containing at least the template specific sequence information from P1 to P2 by promotor controlled transcription, wherein the oligonucleotides P1 and P2 used are not enzymatically ligated together in any step in the process.
Claim 3
Dependent Claim
31 words
The process according to claim 1, further comprising the steps of: hybridizing the transcripts R with oligonucleotides P1 and P2 to form complex K', and transcribing K' by promotor controlled transcription.
Claim 4
Dependent Claim
18 words
The process according to claim 1, wherein the amount of P1 is equal to the amount of P2.
Claim 5
Dependent Claim
15 words
The process according to claim 1, wherein the process is carried out at one temperature.
Claim 6
Dependent Claim
26 words
The process according to claim 1, wherein monoribonucleoside triphosphates which are detectably labelled or capable of being immobilized are incorporated into the transcripts R during transcription.
Claim 7
Dependent Claim
15 words
The process according to claim 1, wherein P2 is immobilized or capable of being immobilized.
Claim 8
Dependent Claim
25 words
The process according to claim 1, wherein the transcripts R formed in step b) contain at least the template specific information from P1 to P2.
Claim 9
Dependent Claim
84 words
A process for the specific detection of a template nucleic acid comprising the steps of: a) hybridizing a template specific promotor oligonucleotide P1 and a template specific oligonucleotide P2 with a template nucleic acid T to form a complex K, b) forming transcripts R containing at least the template specific sequence information from P1 to P2 by promotor controlled transcription, and c) detecting said transcripts R, wherein the oligonucleotides P1 and P2 used are not enzymatically ligated together in any step in the process.
Claim 10
Dependent Claim
84 words
A process for the specific detection of a template nucleic acid comprising the steps of: a) hybridizing a template specific promotor oligonucleotide P1 and a template specific oligonucleotide P2 with a template nucleic acid T to form a complex K, b) forming transcripts R containing at least the template specific sequence information from P1 to P2 by promotor controlled transcription, and c) detecting said transcripts R, wherein the oligonucleotides P1 and P2 used are not enzymatically ligated together in any step in the process.
Claim 11
Dependent Claim
31 words
The process according to claim 8, further comprising the steps of: hybridizing the transcripts R with oligonucleotides P1 and P2 to form complex K', and transcribing K' by promotor controlled transcription.
Claim 12
Dependent Claim
26 words
The process according to claim 8, wherein monoribonucleoside triphosphates which are detectably labeled or capable of being immobilized are incorporated into the transcripts R during transcription.
Claim 13
Dependent Claim
15 words
The process according to claim 8, wherein P2 is immobilized or capable of being immobilized.
References Cited (Backward Citations)
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WO1988010315A1
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EP0310229A1
Cited
EP0320308B1
Cited
EP0329822B1
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EP0373960A2
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EP0427074A2
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EP0427073A2
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-11-28 | 1998-05-30 | 1998-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-11-28 | 2002-05-30 | 2002-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-11-28 | 2006-05-30 | 2006-11-29 | $7700.00 | Window Closed / Lapsed |