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US5367058A
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Modified antibodies with increased affinity
Assignee: Becton Dickinson and Co
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Filing: 1993-08-25
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Granted: 1994-11-22
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Expires: 2013-08-25
Abstract
Abstract The present invention relates to modified antibodies and fragments which display an increased affinity for antigen as compared to the affinity of unmodified antibody for antigen. The most preferred modification is the addition of a thiol group on the surface of the antibody such that a covalent bond forms between the antibody and antigen when they bind to one another.
Inventors
- 👤 J. Bruce Pitner
- 👤 Jr. C. Preston Linn
- 👤 Patrick D. Mize
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:45
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/06; A61K49/08; A61K49/10; A61K49/14; A61K49/16
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
52 words
A thiopropyl modified antibody wherein a thiopropyl moiety is located on the antibody such that a covalent bond is formed between the thiopropyl moiety, which has been reactivated with a thiol-containing group, and an antigen, which has been modified by a maleimide or a thiol-containing group to increase the antigen-antibody binding affinity.
Claim 2
Dependent Claim
52 words
A thiopropyl modified antibody wherein a thiopropyl moiety is located on the antibody such that a covalent bond is formed between the thiopropyl moiety, which has been reactivated with a thiol-containing group, and an antigen, which has been modified by a maleimide or a thiol-containing group to increase the antigen-antibody binding affinity.
Claim 3
Dependent Claim
31 words
The modified antibody of claim 1 wherein the affinity of the modified antibody for the antigen is at least 10-fold greater than the affinity of an unmodified antibody for the antigen.
Claim 4
Dependent Claim
15 words
The modified antibody of claim 1 wherein the covalent bond is a reversible covalent bond.
Claim 5
Dependent Claim
26 words
The modified antibody of claim 1 wherein the antibody is selected from the group consisting of a phosphorylcholine binding antibody and a Streptococcus Group A antibody.
Claim 6
Dependent Claim
14 words
The modified antibody of claim 4 wherein the antibody is a phosphorylcholine binding antibody.
Claim 7
Dependent Claim
15 words
The modified antibody of claim 4 wherein the antibody is a Streptococcus Group A antibody.
References Cited (Backward Citations)
WO1990005749A1
Cited
US5215889A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-11-21 | 1998-05-23 | 1998-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-11-21 | 2002-05-23 | 2002-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-11-21 | 2006-05-23 | 2006-11-22 | $7700.00 | Window Closed / Lapsed |