US5360595A
Expired / Lapsed
US
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Preparation of diagnostic test strips containing tetrazolium salt indicators
Assignee: Bayer Corp
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Filing: 1993-08-19
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Granted: 1994-11-01
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Expires: 2013-08-19
Abstract
Abstract Disclosed is an improved procedure for the manufacture of a diagnostic test device comprising a matrix having uniformly dispersed a tetrazolium salt and a reagent system designed to convert the tetrazolium to its colored formazan upon contacting the matrix with a fluid containing an analyte whose presence and/or concentration is being sought. The procedure involves applying the tetrazolium salt to the matrix from its solution in an organic solvent and, after drying, applying the reagent system to the matrix from its aqueous solution to which has been added hexanesulfonate as wetting agent.
Inventors
- 👤 Douglas E. Bell
- 👤 Amy H. Chu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N31/00
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
146 words
In the method of applying an indicator/reagent system to a matrix carrier, said indicator comprising a tetrazolium salt which, in the presence of the reagent system and a predetermined analyte will undergo a redox reaction resulting in its reduction to a colored formazan, the improvement which comprises: a) contacting the matrix with a solution comprising the tetrazolium salt in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt uniformly dispersed therein; and b) contacting the matrix having the tetrazolium salt uniformly dispersed therein with an aqueous solution of the reagent system which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix without eluting a significant amount of the tetrazolium salt therefrom and provide a matrix having the tetrazolium salt and reagent system uniformly dispersed therein.
Claim 2
Dependent Claim
146 words
In the method of applying an indicator/reagent system to a matrix carrier, said indicator comprising a tetrazolium salt which, in the presence of the reagent system and a predetermined analyte will undergo a redox reaction resulting in its reduction to a colored formazan, the improvement which comprises: a) contacting the matrix with a solution comprising the tetrazolium salt in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt uniformly dispersed therein; and b) contacting the matrix having the tetrazolium salt uniformly dispersed therein with an aqueous solution of the reagent system which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix without eluting a significant amount of the tetrazolium salt therefrom and provide a matrix having the tetrazolium salt and reagent system uniformly dispersed therein.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the reagent system contains hexokinase, adenosine triphosphate, glucose-6-phosphate dehydrogenase, diaphorase and nicotinamideadenine dinucleotide.
Claim 4
Dependent Claim
41 words
The method of claim 1 wherein the tetrazolium salt is represented by the formula: ##STR7## wherein X.sup.⊖ is a counteranion, R 1 and R 3 are substituted or unsubstituted phenyl groups and R 3 is substituted or unsubstituted phenyl or 2-thiazolyl.
Claim 5
Dependent Claim
28 words
The method of claim 1 wherein the matrix material is an absorbant paper, a woven or nonwoven cloth, a glass fiber filter, or a polymeric membrane or film.
Claim 6
Dependent Claim
24 words
The method of claim 4 wherein the matrix material is a porous zwitterionic charged nylon fabric having pore sizes ranging from 0.45 to 0.8μ.
Claim 7
Dependent Claim
23 words
The method of claim 1 wherein the alkyl sulfonate is present in the solution in a concentration of from 0.5% to 1.3% w/v.
Claim 8
Dependent Claim
19 words
The method of claim 6 wherein the alkanesulfonate is present in an amount of from 0.7% to 1.1% w/v.
Claim 9
Independent Claim
152 words
A method of applying an indicator/reagent system to a matrix carrier of a porous zwitterionic charged nylon fabric, said indicator comprising a tetrazolium salt capable of undergoing a redox reaction resulting in its reduction to a colored formazan in the presence of the reagent system and a predetermined analyte which method comprises: a) contacting the matrix with a solution comprising the tetrazolium salt in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt dispersed therein; and b) contacting the matrix having the tetrazolium salt dispersed therein with an aqueous solution of the reagent system comprising hexokinase, adenosine triphosphate, glucose-6-phosphate dehydrogenase, diaphorase and nicotinamideadenine dinucloetide which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix while reducing the amount of tetrazolium salt which is eluted from the matrix from the aqueous solution.
Claim 10
Independent Claim
152 words
A method of applying an indicator/reagent system to a matrix carrier of a porous zwitterionic charged nylon fabric, said indicator comprising a tetrazolium salt capable of undergoing a redox reaction resulting in its reduction to a colored formazan in the presence of the reagent system and a predetermined analyte which method comprises: a) contacting the matrix with a solution comprising the tetrazolium salt in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt dispersed therein; and b) contacting the matrix having the tetrazolium salt dispersed therein with an aqueous solution of the reagent system comprising hexokinase, adenosine triphosphate, glucose-6-phosphate dehydrogenase, diaphorase and nicotinamideadenine dinucloetide which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix while reducing the amount of tetrazolium salt which is eluted from the matrix from the aqueous solution.
Claim 11
Dependent Claim
27 words
The method of claim 8 wherein the alkanesulfonate is hexane sulfonate and is present in the aqueous solution in a concentration of from 0.5% to 1.3% w/v.
Claim 12
Dependent Claim
24 words
The method of claim 9 wherein the hexane sulfonate is present in the aqueous solution in a concentration of from 0.7% to 1.1% w/v.
Claim 13
Dependent Claim
121 words
A diagnostic test device prepared by: a) contacting a matrix carrier with a solution comprising a tetrazolium salt which, in the presence of a reagent system and a predetermined analyte will undergo a redox reaction resulting in its reduction to a colored formazan in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt uniformly dispersed therein; and b) contacting the matrix having the tetrazolium salt uniformly dispersed therein with an aqueous solution of the reagent system which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix to provide a matrix having the tetrazolium salt and reagent system uniformly dispersed therein.
Claim 14
Dependent Claim
121 words
A diagnostic test device prepared by: a) contacting a matrix carrier with a solution comprising a tetrazolium salt which, in the presence of a reagent system and a predetermined analyte will undergo a redox reaction resulting in its reduction to a colored formazan in an organic solvent and drying the matrix to thereby provide a matrix having the tetrazolium salt uniformly dispersed therein; and b) contacting the matrix having the tetrazolium salt uniformly dispersed therein with an aqueous solution of the reagent system which solution contains an alkylsulfonate of 5-7 straight chain carbon atoms and drying the matrix to thereby deposit the reagent system onto the matrix to provide a matrix having the tetrazolium salt and reagent system uniformly dispersed therein.
Claim 15
Dependent Claim
19 words
The device of claim 11 wherein the reagent system contains hexokinase, adenosine triphosphate, glucose-6-phosphate dehydrogenase, diaphorase and nicotinamideadenine dinucleotide.
Claim 16
Dependent Claim
41 words
The device of claim 11 wherein the tetrazolium salt is represented by the formula: ##STR8## wherein X.sup.⊖ is a counteranion, R 1 and R 3 are substituted or unsubstituted phenyl groups and R 3 is substituted or unsubstituted phenyl or 2-thiazolyl.
Claim 17
Dependent Claim
28 words
The device of claim 11 wherein the matrix material is an absorbent paper, a woven or nonwoven cloth, a glass fiber filter, or a polymeric membrane or film.
Claim 18
Dependent Claim
24 words
The device of claim 11 wherein the matrix material is a porous zwitterionic charged nylon fabric having pore sizes ranging from 0.45 to 0.8μ.
Claim 19
Dependent Claim
23 words
The device of claim 11 wherein the alkyl sulfonate is present in the solution in a concentration of from 0.5% to 1.3% w/v.
Claim 20
Dependent Claim
19 words
The device of claim 11 wherein the alkanesulfonate is present in an amount of from 0.7% to 1.1% w/v.
References Cited (Backward Citations)
US4221864A
Cited
US5063153A
Cited
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