US5356801A
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US
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Recombinant DNA encoding a desulfurization biocatalyst
Assignee: BASF Enzymes LLC
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Filing: 1993-07-09
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Granted: 1994-10-18
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Expires: 2013-07-09
Abstract
Abstract This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.
Inventors
- 👤 John Rambosek
- 👤 Chris S. Piddington
- 👤 Brian R. Kovacevich
- 👤 Kevin D. Young
- 👤 Sylvia A. Denome
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 03:46
CPC Classifications
C; C10; C10G; C10G32/00; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12R; C12R2001/00; C12R2001/01
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
25 words
A recombinant DNA molecule from Rhodococcus origin wherein said DNA molecule encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 2
Dependent Claim
25 words
A recombinant DNA molecule from Rhodococcus origin wherein said DNA molecule encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 3
Dependent Claim
20 words
The recombinant DNA molecule of claim 1 wherein the DNA molecule is from a strain of Rhodococcus sp. ATCC 53968.
Claim 4
Dependent Claim
13 words
The recombinant DNA molecule of claim 1 wherein said fossil fuel is petroleum.
Claim 5
Dependent Claim
35 words
A recombinant DNA vector containing a DNA molecule from Rhodococcus origin capable of desulfurizing a fossil fuel wherein said DNA molecule encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 6
Dependent Claim
35 words
A recombinant DNA vector containing a DNA molecule from Rhodococcus origin capable of desulfurizing a fossil fuel wherein said DNA molecule encodes a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 7
Dependent Claim
20 words
The recombinant DNA vector of claim 4 wherein the DNA molecule is from a strain of Rhodococcus sp. ATCC 53968.
Claim 8
Dependent Claim
2 words
Plasmid pTOXI-1.
Claim 9
Dependent Claim
2 words
Plasmid pTOXI-1.
Claim 10
Dependent Claim
2 words
Plasmid pTOXI-2.
Claim 11
Dependent Claim
2 words
Plasmid pTOXI-2.
Claim 12
Dependent Claim
30 words
A transformed microorganism containing a recombinant DNA plasmid containing a DNA molecule of Rhodococcus origin which expresses a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 13
Dependent Claim
30 words
A transformed microorganism containing a recombinant DNA plasmid containing a DNA molecule of Rhodococcus origin which expresses a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 14
Dependent Claim
19 words
The transformed microorganism of claim 8 wherein the DNA molecule is from a strain of Rhodococcus sp. ATCC 53968.
Claim 15
Dependent Claim
28 words
A transformed bacterium containing a DNA molecule from Rhodococcus origin wherein said transformed bacteria expresses a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 16
Dependent Claim
28 words
A transformed bacterium containing a DNA molecule from Rhodococcus origin wherein said transformed bacteria expresses a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules.
Claim 17
Dependent Claim
18 words
The bacterium of claim 10 wherein the DNA molecule is from a strain of Rhodococcus sp. ATCC 53968.
Claim 18
Dependent Claim
16 words
A recombinant DNA vector comprising the nucleic acid sequence set forth in SEQ ID NO: 1.
Claim 19
Dependent Claim
16 words
A recombinant DNA vector comprising the nucleic acid sequence set forth in SEQ ID NO: 1.
Claim 20
Dependent Claim
20 words
A recombinant DNA vector comprising a nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 2.
Claim 21
Dependent Claim
20 words
A recombinant DNA vector comprising a nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 2.
Claim 22
Dependent Claim
20 words
A recombinant DNA vector comprising a nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 3.
Claim 23
Dependent Claim
20 words
A recombinant DNA vector comprising a nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 3.
Claim 24
Dependent Claim
16 words
A recombinant DNA vector comprising the nucleic acid sequence set forth in SEQ ID NO: 4.
Claim 25
Dependent Claim
16 words
A recombinant DNA vector comprising the nucleic acid sequence set forth in SEQ ID NO: 4.
Claim 26
Dependent Claim
20 words
A recombinant DNA vector comprising the nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 5.
Claim 27
Dependent Claim
20 words
A recombinant DNA vector comprising the nucleic acid sequence which encodes the protein set forth in SEQ ID NO: 5.
Claim 28
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 2 [ORF-1].
Claim 29
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 2 [ORF-1].
Claim 30
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 3 [ORF-2].
Claim 31
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 3 [ORF-2].
Claim 32
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 5 [ORF-3].
Claim 33
Dependent Claim
15 words
A DNA sequence which encodes the protein set forth in SEQ ID NO: 5 [ORF-3].
Claim 34
Dependent Claim
22 words
A recombinant DNA molecule encoding a biocatalyst that desulfurizes fossil fuel wherein said molecule hybridizes to a DNA molecule of claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-17 | 1998-04-18 | 1998-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-17 | 2002-04-18 | 2002-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-17 | 2006-04-18 | 2006-10-18 | $7700.00 | Window Closed / Lapsed |