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EP0445896A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Bacterial produced extracts and enzymes for cleavage of organic C-S bonds

Assignee: Gas Technology Institute
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Filing: 1991-01-05
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Granted: —
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Expires: 2011-01-05
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Abstract

Abstract An extract of membrane fragments, an enzyme, and a composition of enzymes associated with cell membranes of Rhodococcus rhodochrous strain ATCC No. 53968 and Bacillus sphaericus strain ATCC No. 53969 which have the ability to selectively react with organic sulfur of sulfur-containing organic carbonaceous material by cleavage or organic C-S bonds.

Inventors

  • 👤 John J. Kilbane Ii

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-04 09:04
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/113; C07K1/1133; C10; C10G; C10G32/00; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P21/00; C12P21/06; C12P3/00; C12R; C12R2001/00; C12R2001/01; C12R2001/07

Claims Analysis

Total Claims: 26
Claim 1
Independent Claim 28 words
An extract comprising membrane fragments of microorganisms selected from the group consisting of Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969, and mixtures thereof.
Claim 2
Dependent Claim 16 words
An extract according to Claim 1 wherein said microorganisms are Rhodococcus rhodochrous strain ATCC No. 53968.
Claim 3
Dependent Claim 16 words
An extract according to Claim 1 wherein said microorganisms are Bacillus sphaericus strain ATCC No. 53969.
Claim 4
Dependent Claim 59 words
An enzyme or composition of enzymes associated with cell membranes of microorganisms selected from the group consisting of Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969, and mixtures thereof, said enzyme or composition of enzymes having the ability to selectively react with organic sulfur of sulfur-containing organic carbonaceous material by cleavage of organic C-S bonds.
Claim 5
Dependent Claim 20 words
An enzyme or composition of enzymes according to Claim 4 wherein said microorganisms are Rhodococcus rhodochrous strain ATCC No. 53968.
Claim 6
Dependent Claim 20 words
An enzyme or composition of enzymes according to Claim 4 wherein said microorganisms are Bacillus sphaericus strain ATCC No. 53969.
Claim 7
Dependent Claim 82 words
A process for reducing the sulfur content of sulfur-containing organic carbonaceous material comprising, contacting a sulfur-containing organic carbonaceous material with a sulfur specific reactant agent selected from the group consisting of an extract comprising membrane fragments, an enzyme associated with a cell membrane, a composition of enzymes associated with a cell membrane, and mixtures thereof, wherein said membrane is from microorganisms selected from the group consisting of Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969, and mixtures thereof.
Claim 8
Dependent Claim 16 words
A process according to Claim 7 wherein said microorganisms are Rhodococcus rhodochrous strain ATCC No. 53968.
Claim 9
Dependent Claim 15 words
A process according to Claim 7 wherein said microorganisms are Bacillus sphaericus strain No. 53969.
Claim 10
Dependent Claim 17 words
A process according to Claim 7 wherein said sulfur specific reactant agent is in an organic media.
Claim 11
Dependent Claim 19 words
A process according to Claim 7 wherein said sulfur specific reactant agent comprises said extract in an organic medium.
Claim 12
Dependent Claim 19 words
A process according to Claim 7 wherein said sulfur specific reactant agent comprises an enzyme in an organic medium.
Claim 13
Dependent Claim 26 words
A process according to Claim 7 wherein said sulfur specific reactant agent comprises a composition of enzymes associated with a cell membrane in an organic medium.
Claim 14
Dependent Claim 19 words
A process according to Claim 7 wherein said contacting is carried out at temperatures about 35° to about 100°C.
Claim 15
Dependent Claim 12 words
A process according to Claim 7 wherein said carbonaceous material is coal.
Claim 16
Dependent Claim 13 words
A process according to Claim 7 wherein said carbonaceous material is hydrocarbon oil.
Claim 17
Dependent Claim 79 words
A process for cleavage of C-S bonds comprising, contacting an organic carbonaceous material having C-S bonds with a sulfur specific reactant agent selected from the group consisting of an extract comprising membrane fragments, an enzyme associated with a cell membrane, a composition of enzymes associated with a cell membrane, and mixtures thereof, wherein said membrane is from microorganisms selected from the group consisting of Rhodococcus rhodochrous strain ATCC No. 53968, Bacillus sphaericus strain ATCC No. 53969, and mixtures thereof.
Claim 18
Dependent Claim 21 words
A process for cleavage of C-S bonds according to Claim 17 wherein said microorganisms are Rhodococcus rhodochrous strain ATCC No. 53968.
Claim 19
Dependent Claim 20 words
A process for cleavage of C-S bonds according to Claim 17 wherein said microorganisms are Bacillus sphaericus strain No. 53969.
Claim 20
Dependent Claim 22 words
A process for cleavage of C-S bonds according to Claim 17 wherein said sulfur specific reactant agent is in an organic medium.
Claim 21
Dependent Claim 24 words
A process for cleavage of C-S bonds according to Claim 17 wherein said sulfur specific reactant agent comprises said extract in an organic medium.
Claim 22
Dependent Claim 24 words
A process for cleavage of C-S bonds according to Claim 17 wherein said sulfur specific reactant agent comprises an enzyme in an organic medium.
Claim 23
Dependent Claim 31 words
A process for cleavage of C-S bonds according to Claim 17 wherein said sulfur specific reactant agent comprises a composition of enzymes associated with a cell membrane in an organic medium.
Claim 24
Dependent Claim 24 words
A process for cleavage of C-S bonds according to Claim 17 wherein said contacting is carried out at temperatures about 35° to about 100°C.
Claim 25
Dependent Claim 17 words
A process for cleavage of C-S bonds according to Claim 17 wherein said carbonaceous material is coal.
Claim 26
Dependent Claim 18 words
A process for cleavage of C-S bonds according to Claim 17 wherein said carbonaceous material is hydrocarbon oil.

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